Molecular cloning of a novel transmembrane protein MOLT expressed by mature oligodendrocytes

Hiroyuki Nomoto, Tomoko Yonezawa, Kouichi Itoh, Katsuhiko Ono, Kiyotaka Yamamoto, Toshitaka Oohashi, Fumio Shiraga, Hiroshi Ohtsuki, Yoshifumi Ninomiya

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

A novel oligodendrocyte (OL)-specific cDNA was isolated from brain capillary endothelial cells and characterized. The cDNA encodes a protein of 1099 amino acids that contains a signal peptide and a transmembrane domain. The protein was expressed in mature OLs in vivo and in vitro cell cultures and was thus designated as mature OL transmembrane protein (MOLT). RT-PCR analysis showed that MOLT mRNA was expressed in brain, lung, pancreas, and testis. A polyclonal antibody raised against a part of the mouse MOLT reacted specifically with multipolar OLs possessing radially oriented processes that penetrated into the gray matter. More cells were detected in the white matter, and these had longitudinally oriented processes. In a rat OL lineage culture system, oligodendrocyte precursor cells did not initially produce MOLT mRNA and protein, but when they begun to differentiate into mature OLs, they started expressing MOLT. Consequently, MOLT may function as OLs become mature and may serve as a cell-surface marker for OL differentiation.

Original languageEnglish
Pages (from-to)231-238
Number of pages8
JournalJournal of biochemistry
Volume134
Issue number2
DOIs
Publication statusPublished - Aug 1 2003

Keywords

  • Differentiation
  • Glial cell
  • Myelination
  • Oligodendrocyte
  • Oligodendrocyte precursor cell

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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