Molecular cloning of a human monocyte-derived neutrophil chemotactic factor (MDNCF) and the induction of MDNCF mRNA by interleukin 1 and tumor necrosis factor

K. Matsushima, K. Morishita, Teizo Yoshimura, S. Lavu, Y. Kobayashi, W. Lew, E. Appella, H. F. Kung, E. J. Leonard, J. J. Oppenheim

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Abstract

The cDNA coding for human monocyte-derived neutrophil-specific chemotactic factor (MDNCF) was cloned from LPS-stimulated human monocyte mRNA. The cDNA sequence codes for a polypeptide consisting of 99 amino acids, including a putative signal sequence. Comparison of the deduced amino acid sequence with the NH2-terminal amino acid sequence of natural MDNCF shows that the mature functional protein comprises 72 amino acids, beginning with serine at residue 28. The deduced amino acid sequence shows striking similarity to several platelet-derived factors, a v-src-induced protein, a growth-regulated gene product (gro), and an IFN-γ inducible protein. The availability of the MDNCD cDNA enabled us to use it as a probe to identify inducers of MDNCD mRNA expression in human PBMC. MDNCD mRNA was increased > 10-fold within 1 h after stimulation with LPS, IL-1, or TNF, but not by IFN-γ, IFN-α, or IL-2. Furthermore, we also determined that LPS, IL-1, and TNF stimulated the mononuclear cells to produce biologically active MDNCF. This observation may account for the in vivo capacity of IL-1 and TNF to induce neutrophil infiltrates.

Original languageEnglish
Pages (from-to)1883-1893
Number of pages11
JournalJournal of Experimental Medicine
Volume167
Issue number6
Publication statusPublished - 1988
Externally publishedYes

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Molecular Cloning
Interleukin-8
Interleukin-1
Amino Acid Sequence
Complementary DNA
Tumor Necrosis Factor-alpha
Messenger RNA
Oncogene Protein pp60(v-src)
Amino Acids
Protein Sorting Signals
Serine
Interleukin-2
Monocytes
Proteins
Neutrophils
Blood Platelets
Peptides
Growth
Genes

ASJC Scopus subject areas

  • Immunology

Cite this

Molecular cloning of a human monocyte-derived neutrophil chemotactic factor (MDNCF) and the induction of MDNCF mRNA by interleukin 1 and tumor necrosis factor. / Matsushima, K.; Morishita, K.; Yoshimura, Teizo; Lavu, S.; Kobayashi, Y.; Lew, W.; Appella, E.; Kung, H. F.; Leonard, E. J.; Oppenheim, J. J.

In: Journal of Experimental Medicine, Vol. 167, No. 6, 1988, p. 1883-1893.

Research output: Contribution to journalArticle

Matsushima, K, Morishita, K, Yoshimura, T, Lavu, S, Kobayashi, Y, Lew, W, Appella, E, Kung, HF, Leonard, EJ & Oppenheim, JJ 1988, 'Molecular cloning of a human monocyte-derived neutrophil chemotactic factor (MDNCF) and the induction of MDNCF mRNA by interleukin 1 and tumor necrosis factor', Journal of Experimental Medicine, vol. 167, no. 6, pp. 1883-1893.
Matsushima, K. ; Morishita, K. ; Yoshimura, Teizo ; Lavu, S. ; Kobayashi, Y. ; Lew, W. ; Appella, E. ; Kung, H. F. ; Leonard, E. J. ; Oppenheim, J. J. / Molecular cloning of a human monocyte-derived neutrophil chemotactic factor (MDNCF) and the induction of MDNCF mRNA by interleukin 1 and tumor necrosis factor. In: Journal of Experimental Medicine. 1988 ; Vol. 167, No. 6. pp. 1883-1893.
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AU - Lew, W.

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