Abstract
The cDNA coding for human monocyte-derived neutrophil-specific chemotactic factor (MDNCF) was cloned from LPS-stimulated human monocyte mRNA. The cDNA sequence codes for a polypeptide consisting of 99 amino acids, including a putative signal sequence. Comparison of the deduced amino acid sequence with the NH2-terminal amino acid sequence of natural MDNCF shows that the mature functional protein comprises 72 amino acids, beginning with serine at residue 28. The deduced amino acid sequence shows striking similarity to several platelet-derived factors, a v-src-induced protein, a growth-regulated gene product (gro), and an IFN-γ inducible protein. The availability of the MDNCD cDNA enabled us to use it as a probe to identify inducers of MDNCD mRNA expression in human PBMC. MDNCD mRNA was increased > 10-fold within 1 h after stimulation with LPS, IL-1, or TNF, but not by IFN-γ, IFN-α, or IL-2. Furthermore, we also determined that LPS, IL-1, and TNF stimulated the mononuclear cells to produce biologically active MDNCF. This observation may account for the in vivo capacity of IL-1 and TNF to induce neutrophil infiltrates.
| Original language | English |
|---|---|
| Pages (from-to) | 1883-1893 |
| Number of pages | 11 |
| Journal | Journal of Experimental Medicine |
| Volume | 167 |
| Issue number | 6 |
| Publication status | Published - 1988 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Immunology
Cite this
Molecular cloning of a human monocyte-derived neutrophil chemotactic factor (MDNCF) and the induction of MDNCF mRNA by interleukin 1 and tumor necrosis factor. / Matsushima, K.; Morishita, K.; Yoshimura, Teizo; Lavu, S.; Kobayashi, Y.; Lew, W.; Appella, E.; Kung, H. F.; Leonard, E. J.; Oppenheim, J. J.
In: Journal of Experimental Medicine, Vol. 167, No. 6, 1988, p. 1883-1893.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Molecular cloning of a human monocyte-derived neutrophil chemotactic factor (MDNCF) and the induction of MDNCF mRNA by interleukin 1 and tumor necrosis factor
AU - Matsushima, K.
AU - Morishita, K.
AU - Yoshimura, Teizo
AU - Lavu, S.
AU - Kobayashi, Y.
AU - Lew, W.
AU - Appella, E.
AU - Kung, H. F.
AU - Leonard, E. J.
AU - Oppenheim, J. J.
PY - 1988
Y1 - 1988
N2 - The cDNA coding for human monocyte-derived neutrophil-specific chemotactic factor (MDNCF) was cloned from LPS-stimulated human monocyte mRNA. The cDNA sequence codes for a polypeptide consisting of 99 amino acids, including a putative signal sequence. Comparison of the deduced amino acid sequence with the NH2-terminal amino acid sequence of natural MDNCF shows that the mature functional protein comprises 72 amino acids, beginning with serine at residue 28. The deduced amino acid sequence shows striking similarity to several platelet-derived factors, a v-src-induced protein, a growth-regulated gene product (gro), and an IFN-γ inducible protein. The availability of the MDNCD cDNA enabled us to use it as a probe to identify inducers of MDNCD mRNA expression in human PBMC. MDNCD mRNA was increased > 10-fold within 1 h after stimulation with LPS, IL-1, or TNF, but not by IFN-γ, IFN-α, or IL-2. Furthermore, we also determined that LPS, IL-1, and TNF stimulated the mononuclear cells to produce biologically active MDNCF. This observation may account for the in vivo capacity of IL-1 and TNF to induce neutrophil infiltrates.
AB - The cDNA coding for human monocyte-derived neutrophil-specific chemotactic factor (MDNCF) was cloned from LPS-stimulated human monocyte mRNA. The cDNA sequence codes for a polypeptide consisting of 99 amino acids, including a putative signal sequence. Comparison of the deduced amino acid sequence with the NH2-terminal amino acid sequence of natural MDNCF shows that the mature functional protein comprises 72 amino acids, beginning with serine at residue 28. The deduced amino acid sequence shows striking similarity to several platelet-derived factors, a v-src-induced protein, a growth-regulated gene product (gro), and an IFN-γ inducible protein. The availability of the MDNCD cDNA enabled us to use it as a probe to identify inducers of MDNCD mRNA expression in human PBMC. MDNCD mRNA was increased > 10-fold within 1 h after stimulation with LPS, IL-1, or TNF, but not by IFN-γ, IFN-α, or IL-2. Furthermore, we also determined that LPS, IL-1, and TNF stimulated the mononuclear cells to produce biologically active MDNCF. This observation may account for the in vivo capacity of IL-1 and TNF to induce neutrophil infiltrates.
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M3 - Article
VL - 167
SP - 1883
EP - 1893
JO - Journal of Experimental Medicine
JF - Journal of Experimental Medicine
SN - 0022-1007
IS - 6
ER -