Molecular cloning of a cDNA encoding vitellogenesis-inhibiting hormone in the whiteleg shrimp Litopenaeus vannamei and preparation of its recombinant peptide using an E. coli expression system

Naoaki Tsutsui, Tsuyoshi Ohira, Tomoyuki Okutsu, Junpei Shinji, Sun Hye Bae, Bong Jung Kang, Marcy N. Wilder

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The cDNA encoding a precursor of Liv-SGP-G, the most abundant crustacean hyperglycemic hormone-family peptide in the sinus gland of the whiteleg shrimp Litopenaeus vannamei, was cloned by RT-PCR coupled with 5′- and 3′-RACE. The determined cDNA sequence consisted of 621 nucleotides comprising a 69-bp 5′-untranslated region, a 357-bp open reading frame, and a 195-bp 3′-untranslated region. The deduced sequence of 72 amino acid residues corresponding to mature Liv-SGP-G was completely identical to that of natural Liv-SGP-G, determined in our previous study. The recombinant Liv-SGP-G was thereafter heterologously expressed in Escherichia coli, and its vitellogenesis-inhibiting activity in ex vivo cultured ovarian fragments was examined. The recombinant peptides inhibited vitellogenin gene expression with almost the same efficacy as that of natural Liv-SGP-G purified from sinus glands.

Original languageEnglish
Pages (from-to)357-365
Number of pages9
JournalFisheries Science
Volume79
Issue number3
DOIs
Publication statusPublished - May 2013

Fingerprint

vitellogenesis
Litopenaeus vannamei
sinuses
peptide
hormone
molecular cloning
hormones
peptides
Escherichia coli
vitellogenin
5' untranslated regions
3' untranslated regions
gene expression
crustacean
open reading frames
amino acid
Crustacea
nucleotides
reverse transcriptase polymerase chain reaction
amino acids

Keywords

  • Crustacean hyperglycemic hormone family
  • Litopenaeus vannamei
  • Vitellogenesis-inhibiting hormone
  • Vitellogenin gene
  • Whiteleg shrimp

ASJC Scopus subject areas

  • Aquatic Science

Cite this

Molecular cloning of a cDNA encoding vitellogenesis-inhibiting hormone in the whiteleg shrimp Litopenaeus vannamei and preparation of its recombinant peptide using an E. coli expression system. / Tsutsui, Naoaki; Ohira, Tsuyoshi; Okutsu, Tomoyuki; Shinji, Junpei; Bae, Sun Hye; Kang, Bong Jung; Wilder, Marcy N.

In: Fisheries Science, Vol. 79, No. 3, 05.2013, p. 357-365.

Research output: Contribution to journalArticle

Tsutsui, Naoaki ; Ohira, Tsuyoshi ; Okutsu, Tomoyuki ; Shinji, Junpei ; Bae, Sun Hye ; Kang, Bong Jung ; Wilder, Marcy N. / Molecular cloning of a cDNA encoding vitellogenesis-inhibiting hormone in the whiteleg shrimp Litopenaeus vannamei and preparation of its recombinant peptide using an E. coli expression system. In: Fisheries Science. 2013 ; Vol. 79, No. 3. pp. 357-365.
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