Abstract
Chilo suppressalis transferrin cDNA was cloned by reverse transcription polymerase chain reaction (RT-PCR). The clone (2,248 bp) encodes a protein of 644 amino acids with a calculated molecular weight of 71,805 Da. The deduced amino acid sequence showed significant homology with known insect transferrins from Bombyx mori (82%), Manduca sexta (77%), Blaberus discoidalis (43%), Riptortus clavatus (42%), Aedes aegypti (36%), Sarcophaga peregrina (32%), Drosophila melanogaster (33%) and D. silvestris (32%). Transferrin expression was examined by RNA gel blot analysis using diapausing and non-diapausing larvae of C. suppressalis. As a result, higher levels of expression were observed in diapausing larvae.
| Original language | English |
|---|---|
| Pages (from-to) | 463-468 |
| Number of pages | 6 |
| Journal | Applied Entomology and Zoology |
| Volume | 39 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - Aug 2004 |
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Keywords
- Larval diapause
- The rice stem borer
- Transferrin
ASJC Scopus subject areas
- Insect Science
Cite this
Molecular cloning, nucleotide sequence and gene expression of a transferrin gene from the rice stem borer, Chilo suppressalis Walker (Lepidoptera : Crambidae). / Sonoda, Shoji; Maruyama, Tetsuya; Izumi, Yohei; Yoshida, Hideya; Tsumuki, Hisaaki.
In: Applied Entomology and Zoology, Vol. 39, No. 3, 08.2004, p. 463-468.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Molecular cloning, nucleotide sequence and gene expression of a transferrin gene from the rice stem borer, Chilo suppressalis Walker (Lepidoptera
T2 - Crambidae)
AU - Sonoda, Shoji
AU - Maruyama, Tetsuya
AU - Izumi, Yohei
AU - Yoshida, Hideya
AU - Tsumuki, Hisaaki
PY - 2004/8
Y1 - 2004/8
N2 - Chilo suppressalis transferrin cDNA was cloned by reverse transcription polymerase chain reaction (RT-PCR). The clone (2,248 bp) encodes a protein of 644 amino acids with a calculated molecular weight of 71,805 Da. The deduced amino acid sequence showed significant homology with known insect transferrins from Bombyx mori (82%), Manduca sexta (77%), Blaberus discoidalis (43%), Riptortus clavatus (42%), Aedes aegypti (36%), Sarcophaga peregrina (32%), Drosophila melanogaster (33%) and D. silvestris (32%). Transferrin expression was examined by RNA gel blot analysis using diapausing and non-diapausing larvae of C. suppressalis. As a result, higher levels of expression were observed in diapausing larvae.
AB - Chilo suppressalis transferrin cDNA was cloned by reverse transcription polymerase chain reaction (RT-PCR). The clone (2,248 bp) encodes a protein of 644 amino acids with a calculated molecular weight of 71,805 Da. The deduced amino acid sequence showed significant homology with known insect transferrins from Bombyx mori (82%), Manduca sexta (77%), Blaberus discoidalis (43%), Riptortus clavatus (42%), Aedes aegypti (36%), Sarcophaga peregrina (32%), Drosophila melanogaster (33%) and D. silvestris (32%). Transferrin expression was examined by RNA gel blot analysis using diapausing and non-diapausing larvae of C. suppressalis. As a result, higher levels of expression were observed in diapausing larvae.
KW - Larval diapause
KW - The rice stem borer
KW - Transferrin
UR - http://www.scopus.com/inward/record.url?scp=8344276855&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=8344276855&partnerID=8YFLogxK
U2 - 10.1303/aez.2004.463
DO - 10.1303/aez.2004.463
M3 - Article
AN - SCOPUS:8344276855
VL - 39
SP - 463
EP - 468
JO - Applied Entomology and Zoology
JF - Applied Entomology and Zoology
SN - 0003-6862
IS - 3
ER -