Molecular cloning, nucleotide sequence and gene expression of a transferrin gene from the rice stem borer, Chilo suppressalis Walker (Lepidoptera

Crambidae)

Shoji Sonoda, Tetsuya Maruyama, Yohei Izumi, Hideya Yoshida, Hisaaki Tsumuki

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Chilo suppressalis transferrin cDNA was cloned by reverse transcription polymerase chain reaction (RT-PCR). The clone (2,248 bp) encodes a protein of 644 amino acids with a calculated molecular weight of 71,805 Da. The deduced amino acid sequence showed significant homology with known insect transferrins from Bombyx mori (82%), Manduca sexta (77%), Blaberus discoidalis (43%), Riptortus clavatus (42%), Aedes aegypti (36%), Sarcophaga peregrina (32%), Drosophila melanogaster (33%) and D. silvestris (32%). Transferrin expression was examined by RNA gel blot analysis using diapausing and non-diapausing larvae of C. suppressalis. As a result, higher levels of expression were observed in diapausing larvae.

Original languageEnglish
Pages (from-to)463-468
Number of pages6
JournalApplied Entomology and Zoology
Volume39
Issue number3
DOIs
Publication statusPublished - Aug 2004

Fingerprint

Chilo suppressalis
Crambidae
transferrin
molecular cloning
Lepidoptera
nucleotide sequences
gene expression
Blaberus discoidalis
Sarcophaga peregrina
Riptortus clavatus
genes
larvae
Manduca sexta
Bombyx mori
Aedes aegypti
Drosophila melanogaster
amino acid sequences
reverse transcriptase polymerase chain reaction
gels
RNA

Keywords

  • Larval diapause
  • The rice stem borer
  • Transferrin

ASJC Scopus subject areas

  • Insect Science

Cite this

Molecular cloning, nucleotide sequence and gene expression of a transferrin gene from the rice stem borer, Chilo suppressalis Walker (Lepidoptera : Crambidae). / Sonoda, Shoji; Maruyama, Tetsuya; Izumi, Yohei; Yoshida, Hideya; Tsumuki, Hisaaki.

In: Applied Entomology and Zoology, Vol. 39, No. 3, 08.2004, p. 463-468.

Research output: Contribution to journalArticle

Sonoda, Shoji ; Maruyama, Tetsuya ; Izumi, Yohei ; Yoshida, Hideya ; Tsumuki, Hisaaki. / Molecular cloning, nucleotide sequence and gene expression of a transferrin gene from the rice stem borer, Chilo suppressalis Walker (Lepidoptera : Crambidae). In: Applied Entomology and Zoology. 2004 ; Vol. 39, No. 3. pp. 463-468.
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