Molecular cloning and tumour suppressor function analysis of canine REIC/Dkk-3 in mammary gland tumours

Kazuhiko Ochiai, Masami Watanabe, Daigo Azakami, Masaki Michishita, Yasunaga Yoshikawa, Chihiro Udagawa, Pornphimon Metheenukul, Thippayarat Chahomchuen, Hiroshi Aoki, Hiromi Kumon, Masami Morimatsu, Toshinori Omi

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

REIC/Dkk-3, a member of the human Dickkopf (Dkk) family, plays a role as a suppressor of growth in several human cancers. In this study, the tumour suppression function of canine REIC/Dkk-3 was investigated. The full-length open reading frame of the canine REIC/Dkk-3 homologue was cloned and the tissue distribution of REIC/Dkk-3 mRNA was determined, along with the subcellular localisation of the REIC/Dkk-3 protein in canine cancer cell lines. Expression of REIC/Dkk-3 was lower in mammary gland tumours and in canine mammary carcinoma cell lines than in normal mammary gland tissue. Overexpression of REIC/Dkk-3 induced apoptosis in canine mammary carcinoma cell lines. These results show that expression of REIC/Dkk-3 is downregulated in canine mammary tumours and that one of the functions of this gene is induction of apoptosis.

Original languageEnglish
Pages (from-to)769-775
Number of pages7
JournalVeterinary Journal
Volume197
Issue number3
DOIs
Publication statusPublished - Sep 2013

Fingerprint

mammary neoplasms (animal)
Molecular Cloning
Human Mammary Glands
Canidae
molecular cloning
Breast Neoplasms
neoplasms
dogs
Neoplasms
cell lines
Cell Line
breast neoplasms
apoptosis
Apoptosis
human growth
tissue distribution
Tissue Distribution
mammary glands
Open Reading Frames
open reading frames

Keywords

  • Canine
  • Mammary gland tumours
  • REIC/Dkk-3
  • Tumour suppressor gene

ASJC Scopus subject areas

  • Animal Science and Zoology
  • veterinary(all)

Cite this

Molecular cloning and tumour suppressor function analysis of canine REIC/Dkk-3 in mammary gland tumours. / Ochiai, Kazuhiko; Watanabe, Masami; Azakami, Daigo; Michishita, Masaki; Yoshikawa, Yasunaga; Udagawa, Chihiro; Metheenukul, Pornphimon; Chahomchuen, Thippayarat; Aoki, Hiroshi; Kumon, Hiromi; Morimatsu, Masami; Omi, Toshinori.

In: Veterinary Journal, Vol. 197, No. 3, 09.2013, p. 769-775.

Research output: Contribution to journalArticle

Ochiai, K, Watanabe, M, Azakami, D, Michishita, M, Yoshikawa, Y, Udagawa, C, Metheenukul, P, Chahomchuen, T, Aoki, H, Kumon, H, Morimatsu, M & Omi, T 2013, 'Molecular cloning and tumour suppressor function analysis of canine REIC/Dkk-3 in mammary gland tumours', Veterinary Journal, vol. 197, no. 3, pp. 769-775. https://doi.org/10.1016/j.tvjl.2013.04.024
Ochiai, Kazuhiko ; Watanabe, Masami ; Azakami, Daigo ; Michishita, Masaki ; Yoshikawa, Yasunaga ; Udagawa, Chihiro ; Metheenukul, Pornphimon ; Chahomchuen, Thippayarat ; Aoki, Hiroshi ; Kumon, Hiromi ; Morimatsu, Masami ; Omi, Toshinori. / Molecular cloning and tumour suppressor function analysis of canine REIC/Dkk-3 in mammary gland tumours. In: Veterinary Journal. 2013 ; Vol. 197, No. 3. pp. 769-775.
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