Molecular cloning and tumour suppressor function analysis of canine REIC/Dkk-3 in mammary gland tumours

Kazuhiko Ochiai, Masami Watanabe, Daigo Azakami, Masaki Michishita, Yasunaga Yoshikawa, Chihiro Udagawa, Pornphimon Metheenukul, Thippayarat Chahomchuen, Hiroshi Aoki, Hiromi Kumon, Masami Morimatsu, Toshinori Omi

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

REIC/Dkk-3, a member of the human Dickkopf (Dkk) family, plays a role as a suppressor of growth in several human cancers. In this study, the tumour suppression function of canine REIC/Dkk-3 was investigated. The full-length open reading frame of the canine REIC/Dkk-3 homologue was cloned and the tissue distribution of REIC/Dkk-3 mRNA was determined, along with the subcellular localisation of the REIC/Dkk-3 protein in canine cancer cell lines. Expression of REIC/Dkk-3 was lower in mammary gland tumours and in canine mammary carcinoma cell lines than in normal mammary gland tissue. Overexpression of REIC/Dkk-3 induced apoptosis in canine mammary carcinoma cell lines. These results show that expression of REIC/Dkk-3 is downregulated in canine mammary tumours and that one of the functions of this gene is induction of apoptosis.

Original languageEnglish
Pages (from-to)769-775
Number of pages7
JournalVeterinary Journal
Volume197
Issue number3
DOIs
Publication statusPublished - Sep 1 2013

Keywords

  • Canine
  • Mammary gland tumours
  • REIC/Dkk-3
  • Tumour suppressor gene

ASJC Scopus subject areas

  • Animal Science and Zoology
  • veterinary(all)

Fingerprint Dive into the research topics of 'Molecular cloning and tumour suppressor function analysis of canine REIC/Dkk-3 in mammary gland tumours'. Together they form a unique fingerprint.

  • Cite this

    Ochiai, K., Watanabe, M., Azakami, D., Michishita, M., Yoshikawa, Y., Udagawa, C., Metheenukul, P., Chahomchuen, T., Aoki, H., Kumon, H., Morimatsu, M., & Omi, T. (2013). Molecular cloning and tumour suppressor function analysis of canine REIC/Dkk-3 in mammary gland tumours. Veterinary Journal, 197(3), 769-775. https://doi.org/10.1016/j.tvjl.2013.04.024