Molecular cloning and sequence analysis of antigen gene tdpA of Treponema denticola

M. Miyamoto, S. Noji, Susumu Kokeguchi, K. Kato, H. Kurihara, Y. Murayama, S. Taniguchi

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

We isolated and characterized an immunogenic protein of an oral spirochete, Treponema denticola Johnson. A genomic DNA library constructed with bacteriophage lambda EMBL3 as a vector was immunologically screened with a rabbit antiserum against the whole cells. Using Western immunoblot analysis, we found a particular clone encoding an antigen with a molecular weight of 53,000; we designated the antigen as T. denticola protein A (TdpA). Complete sequence determination revealed an open reading frame of 1,419 bp and a signal peptide sequence that was homologous to that of bacterial lipoprotein. Southern hybridization analysis revealed that the tdpA gene is highly conserved in six tested strains of T. denticola species. Furthermore, we found that sera from some periodontitis patients contained antibody against the TdpA protein, although the reactivities of the antibodies varied among individuals.

Original languageEnglish
Pages (from-to)1941-1947
Number of pages7
JournalInfection and Immunity
Volume59
Issue number6
Publication statusPublished - 1991

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Treponema denticola
Molecular Cloning
Sequence Analysis
Antigens
Staphylococcal Protein A
Genes
Bacteriophage lambda
Spirochaetales
Genomic Library
Antibodies
Periodontitis
Protein Sorting Signals
Gene Library
Open Reading Frames
Lipoproteins
Immune Sera
Proteins
Clone Cells
Molecular Weight
Western Blotting

ASJC Scopus subject areas

  • Immunology

Cite this

Miyamoto, M., Noji, S., Kokeguchi, S., Kato, K., Kurihara, H., Murayama, Y., & Taniguchi, S. (1991). Molecular cloning and sequence analysis of antigen gene tdpA of Treponema denticola. Infection and Immunity, 59(6), 1941-1947.

Molecular cloning and sequence analysis of antigen gene tdpA of Treponema denticola. / Miyamoto, M.; Noji, S.; Kokeguchi, Susumu; Kato, K.; Kurihara, H.; Murayama, Y.; Taniguchi, S.

In: Infection and Immunity, Vol. 59, No. 6, 1991, p. 1941-1947.

Research output: Contribution to journalArticle

Miyamoto, M, Noji, S, Kokeguchi, S, Kato, K, Kurihara, H, Murayama, Y & Taniguchi, S 1991, 'Molecular cloning and sequence analysis of antigen gene tdpA of Treponema denticola', Infection and Immunity, vol. 59, no. 6, pp. 1941-1947.
Miyamoto M, Noji S, Kokeguchi S, Kato K, Kurihara H, Murayama Y et al. Molecular cloning and sequence analysis of antigen gene tdpA of Treponema denticola. Infection and Immunity. 1991;59(6):1941-1947.
Miyamoto, M. ; Noji, S. ; Kokeguchi, Susumu ; Kato, K. ; Kurihara, H. ; Murayama, Y. ; Taniguchi, S. / Molecular cloning and sequence analysis of antigen gene tdpA of Treponema denticola. In: Infection and Immunity. 1991 ; Vol. 59, No. 6. pp. 1941-1947.
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