Abstract
In this study, we identified a gene encoding tomato ENGase (Endo-LE) using the gene information of rice ENGase, and expressed the Endo-LE protein in Escherichia coli. The substrate specificity of the recombinant Endo-LE was the same as that of the native enzyme, showing strong activity towards the highmannose type N-glycans with the Manβ1-2Manβ1- 3Manβ1- 4GlcNAcβ1-4GlcNAc unit. Real-time PCR analysis revealed that the gene expression of Endo-LE did not vary significantly with the tomato ripening process, indicating that Endo-LE activity is ubiquitously expressed.
| Original language | English |
|---|---|
| Pages (from-to) | 461-464 |
| Number of pages | 4 |
| Journal | Bioscience, Biotechnology and Biochemistry |
| Volume | 73 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Apr 30 2009 |
Keywords
- De-N-glycosylation
- Endo-β-N-acetylglucosaminidase
- Free Nglycans
- Lycopersicon esculentu
- Tomato fruit ripening
ASJC Scopus subject areas
- Biotechnology
- Analytical Chemistry
- Biochemistry
- Applied Microbiology and Biotechnology
- Molecular Biology
- Organic Chemistry
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Nakamura, K., Inoue, M., Maeda, M., Nakano, R., Hosoi, K., Fujiyama, K., & Kimura, Y. (2009). Molecular cloning and gene expression analysis of tomato endo-β-n-acetylglucosaminidase, an endoglycosidase involved in the production of high-mannose Type Free N-Glycans during tomato fruit ripening. Bioscience, Biotechnology and Biochemistry, 73(2), 461-464. https://doi.org/10.1271/bbb.80889