Molecular cloning and gene expression analysis of tomato endo-β-n-acetylglucosaminidase, an endoglycosidase involved in the production of high-mannose Type Free N-Glycans during tomato fruit ripening

Kosuke Nakamura; Masami Inoue; Megumi Maeda; Ryohei Nakano; Katsutoshi Hosoi; Kazuhito Fujiyama; Yoshinobu Kimura

doi:10.1271/bbb.80889

Molecular cloning and gene expression analysis of tomato endo-β-n-acetylglucosaminidase, an endoglycosidase involved in the production of high-mannose Type Free N-Glycans during tomato fruit ripening

Kosuke Nakamura, Masami Inoue, Megumi Maeda, Ryohei Nakano, Katsutoshi Hosoi, Kazuhito Fujiyama, Yoshinobu Kimura

Research output: Contribution to journal › Article

13 Citations (Scopus)

Abstract

In this study, we identified a gene encoding tomato ENGase (Endo-LE) using the gene information of rice ENGase, and expressed the Endo-LE protein in Escherichia coli. The substrate specificity of the recombinant Endo-LE was the same as that of the native enzyme, showing strong activity towards the highmannose type N-glycans with the Manβ1-2Manβ1- 3Manβ1- 4GlcNAcβ1-4GlcNAc unit. Real-time PCR analysis revealed that the gene expression of Endo-LE did not vary significantly with the tomato ripening process, indicating that Endo-LE activity is ubiquitously expressed.

Original language	English
Pages (from-to)	461-464
Number of pages	4
Journal	Bioscience, Biotechnology and Biochemistry
Volume	73
Issue number	2
DOIs	https://doi.org/10.1271/bbb.80889
Publication status	Published - 2009

Fingerprint

Keywords

De-N-glycosylation
Endo-β-N-acetylglucosaminidase
Free Nglycans
Lycopersicon esculentu
Tomato fruit ripening

ASJC Scopus subject areas

Biotechnology
Biochemistry
Molecular Biology
Applied Microbiology and Biotechnology
Analytical Chemistry
Organic Chemistry
Medicine(all)

Cite this

Nakamura, K., Inoue, M., Maeda, M., Nakano, R., Hosoi, K., Fujiyama, K., & Kimura, Y. (2009). Molecular cloning and gene expression analysis of tomato endo-β-n-acetylglucosaminidase, an endoglycosidase involved in the production of high-mannose Type Free N-Glycans during tomato fruit ripening. Bioscience, Biotechnology and Biochemistry, 73(2), 461-464. https://doi.org/10.1271/bbb.80889

Molecular cloning and gene expression analysis of tomato endo-β-n-acetylglucosaminidase, an endoglycosidase involved in the production of high-mannose Type Free N-Glycans during tomato fruit ripening. / Nakamura, Kosuke; Inoue, Masami; Maeda, Megumi; Nakano, Ryohei; Hosoi, Katsutoshi; Fujiyama, Kazuhito; Kimura, Yoshinobu.

In: Bioscience, Biotechnology and Biochemistry, Vol. 73, No. 2, 2009, p. 461-464.

Research output: Contribution to journal › Article

Nakamura, K, Inoue, M, Maeda, M, Nakano, R, Hosoi, K, Fujiyama, K & Kimura, Y 2009, 'Molecular cloning and gene expression analysis of tomato endo-β-n-acetylglucosaminidase, an endoglycosidase involved in the production of high-mannose Type Free N-Glycans during tomato fruit ripening', Bioscience, Biotechnology and Biochemistry, vol. 73, no. 2, pp. 461-464. https://doi.org/10.1271/bbb.80889

Nakamura, Kosuke ; Inoue, Masami ; Maeda, Megumi ; Nakano, Ryohei ; Hosoi, Katsutoshi ; Fujiyama, Kazuhito ; Kimura, Yoshinobu. / Molecular cloning and gene expression analysis of tomato endo-β-n-acetylglucosaminidase, an endoglycosidase involved in the production of high-mannose Type Free N-Glycans during tomato fruit ripening. In: Bioscience, Biotechnology and Biochemistry. 2009 ; Vol. 73, No. 2. pp. 461-464.

@article{a2559d571be3420389eddb5858e0066e,

title = "Molecular cloning and gene expression analysis of tomato endo-β-n-acetylglucosaminidase, an endoglycosidase involved in the production of high-mannose Type Free N-Glycans during tomato fruit ripening",

abstract = "In this study, we identified a gene encoding tomato ENGase (Endo-LE) using the gene information of rice ENGase, and expressed the Endo-LE protein in Escherichia coli. The substrate specificity of the recombinant Endo-LE was the same as that of the native enzyme, showing strong activity towards the highmannose type N-glycans with the Manβ1-2Manβ1- 3Manβ1- 4GlcNAcβ1-4GlcNAc unit. Real-time PCR analysis revealed that the gene expression of Endo-LE did not vary significantly with the tomato ripening process, indicating that Endo-LE activity is ubiquitously expressed.",

keywords = "De-N-glycosylation, Endo-β-N-acetylglucosaminidase, Free Nglycans, Lycopersicon esculentu, Tomato fruit ripening",

author = "Kosuke Nakamura and Masami Inoue and Megumi Maeda and Ryohei Nakano and Katsutoshi Hosoi and Kazuhito Fujiyama and Yoshinobu Kimura",

year = "2009",

doi = "10.1271/bbb.80889",

language = "English",

volume = "73",

pages = "461--464",

journal = "Bioscience, Biotechnology and Biochemistry",

issn = "0916-8451",

publisher = "Japan Society for Bioscience Biotechnology and Agrochemistry",

number = "2",

}

TY - JOUR

T1 - Molecular cloning and gene expression analysis of tomato endo-β-n-acetylglucosaminidase, an endoglycosidase involved in the production of high-mannose Type Free N-Glycans during tomato fruit ripening

AU - Nakamura, Kosuke

AU - Inoue, Masami

AU - Maeda, Megumi

AU - Nakano, Ryohei

AU - Hosoi, Katsutoshi

AU - Fujiyama, Kazuhito

AU - Kimura, Yoshinobu

PY - 2009

Y1 - 2009

N2 - In this study, we identified a gene encoding tomato ENGase (Endo-LE) using the gene information of rice ENGase, and expressed the Endo-LE protein in Escherichia coli. The substrate specificity of the recombinant Endo-LE was the same as that of the native enzyme, showing strong activity towards the highmannose type N-glycans with the Manβ1-2Manβ1- 3Manβ1- 4GlcNAcβ1-4GlcNAc unit. Real-time PCR analysis revealed that the gene expression of Endo-LE did not vary significantly with the tomato ripening process, indicating that Endo-LE activity is ubiquitously expressed.

AB - In this study, we identified a gene encoding tomato ENGase (Endo-LE) using the gene information of rice ENGase, and expressed the Endo-LE protein in Escherichia coli. The substrate specificity of the recombinant Endo-LE was the same as that of the native enzyme, showing strong activity towards the highmannose type N-glycans with the Manβ1-2Manβ1- 3Manβ1- 4GlcNAcβ1-4GlcNAc unit. Real-time PCR analysis revealed that the gene expression of Endo-LE did not vary significantly with the tomato ripening process, indicating that Endo-LE activity is ubiquitously expressed.

KW - De-N-glycosylation

KW - Endo-β-N-acetylglucosaminidase

KW - Free Nglycans

KW - Lycopersicon esculentu

KW - Tomato fruit ripening

UR - http://www.scopus.com/inward/record.url?scp=65249155427&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=65249155427&partnerID=8YFLogxK

U2 - 10.1271/bbb.80889

DO - 10.1271/bbb.80889

M3 - Article

C2 - 19202265

AN - SCOPUS:65249155427

VL - 73

SP - 461

EP - 464

JO - Bioscience, Biotechnology and Biochemistry

JF - Bioscience, Biotechnology and Biochemistry

SN - 0916-8451

IS - 2

ER -

Access to Document

10.1271/bbb.80889