Molecular cloning and gene expression analysis of tomato endo-β-n-acetylglucosaminidase, an endoglycosidase involved in the production of high-mannose Type Free N-Glycans during tomato fruit ripening

Kosuke Nakamura; Masami Inoue; Megumi Maeda; Ryohei Nakano; Katsutoshi Hosoi; Kazuhito Fujiyama; Yoshinobu Kimura

doi:10.1271/bbb.80889

Molecular cloning and gene expression analysis of tomato endo-β-n-acetylglucosaminidase, an endoglycosidase involved in the production of high-mannose Type Free N-Glycans during tomato fruit ripening

Kosuke Nakamura, Masami Inoue, Megumi Maeda, Ryohei Nakano, Katsutoshi Hosoi, Kazuhito Fujiyama, Yoshinobu Kimura

Research output: Contribution to journal › Article › peer-review

16 Citations (Scopus)

Abstract

In this study, we identified a gene encoding tomato ENGase (Endo-LE) using the gene information of rice ENGase, and expressed the Endo-LE protein in Escherichia coli. The substrate specificity of the recombinant Endo-LE was the same as that of the native enzyme, showing strong activity towards the highmannose type N-glycans with the Manβ1-2Manβ1- 3Manβ1- 4GlcNAcβ1-4GlcNAc unit. Real-time PCR analysis revealed that the gene expression of Endo-LE did not vary significantly with the tomato ripening process, indicating that Endo-LE activity is ubiquitously expressed.

Original language	English
Pages (from-to)	461-464
Number of pages	4
Journal	Bioscience, Biotechnology and Biochemistry
Volume	73
Issue number	2
DOIs	https://doi.org/10.1271/bbb.80889
Publication status	Published - Apr 30 2009

Keywords

De-N-glycosylation
Endo-β-N-acetylglucosaminidase
Free Nglycans
Lycopersicon esculentu
Tomato fruit ripening

ASJC Scopus subject areas

Biotechnology
Analytical Chemistry
Biochemistry
Applied Microbiology and Biotechnology
Molecular Biology
Organic Chemistry

Access to Document

10.1271/bbb.80889

Cite this

Nakamura, K., Inoue, M., Maeda, M., Nakano, R., Hosoi, K., Fujiyama, K., & Kimura, Y. (2009). Molecular cloning and gene expression analysis of tomato endo-β-n-acetylglucosaminidase, an endoglycosidase involved in the production of high-mannose Type Free N-Glycans during tomato fruit ripening. Bioscience, Biotechnology and Biochemistry, 73(2), 461-464. https://doi.org/10.1271/bbb.80889

Molecular cloning and gene expression analysis of tomato endo-β-n-acetylglucosaminidase, an endoglycosidase involved in the production of high-mannose Type Free N-Glycans during tomato fruit ripening. / Nakamura, Kosuke; Inoue, Masami; Maeda, Megumi et al.

In: Bioscience, Biotechnology and Biochemistry, Vol. 73, No. 2, 30.04.2009, p. 461-464.

Research output: Contribution to journal › Article › peer-review

Nakamura, K, Inoue, M, Maeda, M, Nakano, R, Hosoi, K, Fujiyama, K & Kimura, Y 2009, 'Molecular cloning and gene expression analysis of tomato endo-β-n-acetylglucosaminidase, an endoglycosidase involved in the production of high-mannose Type Free N-Glycans during tomato fruit ripening', Bioscience, Biotechnology and Biochemistry, vol. 73, no. 2, pp. 461-464. https://doi.org/10.1271/bbb.80889

@article{a2559d571be3420389eddb5858e0066e,

title = "Molecular cloning and gene expression analysis of tomato endo-β-n-acetylglucosaminidase, an endoglycosidase involved in the production of high-mannose Type Free N-Glycans during tomato fruit ripening",

abstract = "In this study, we identified a gene encoding tomato ENGase (Endo-LE) using the gene information of rice ENGase, and expressed the Endo-LE protein in Escherichia coli. The substrate specificity of the recombinant Endo-LE was the same as that of the native enzyme, showing strong activity towards the highmannose type N-glycans with the Manβ1-2Manβ1- 3Manβ1- 4GlcNAcβ1-4GlcNAc unit. Real-time PCR analysis revealed that the gene expression of Endo-LE did not vary significantly with the tomato ripening process, indicating that Endo-LE activity is ubiquitously expressed.",

keywords = "De-N-glycosylation, Endo-β-N-acetylglucosaminidase, Free Nglycans, Lycopersicon esculentu, Tomato fruit ripening",

author = "Kosuke Nakamura and Masami Inoue and Megumi Maeda and Ryohei Nakano and Katsutoshi Hosoi and Kazuhito Fujiyama and Yoshinobu Kimura",

year = "2009",

month = apr,

day = "30",

doi = "10.1271/bbb.80889",

language = "English",

volume = "73",

pages = "461--464",

journal = "Bioscience, Biotechnology and Biochemistry",

issn = "0916-8451",

publisher = "Japan Society for Bioscience Biotechnology and Agrochemistry",

number = "2",

}

TY - JOUR

T1 - Molecular cloning and gene expression analysis of tomato endo-β-n-acetylglucosaminidase, an endoglycosidase involved in the production of high-mannose Type Free N-Glycans during tomato fruit ripening

AU - Nakamura, Kosuke

AU - Inoue, Masami

AU - Maeda, Megumi

AU - Nakano, Ryohei

AU - Hosoi, Katsutoshi

AU - Fujiyama, Kazuhito

AU - Kimura, Yoshinobu

PY - 2009/4/30

Y1 - 2009/4/30

N2 - In this study, we identified a gene encoding tomato ENGase (Endo-LE) using the gene information of rice ENGase, and expressed the Endo-LE protein in Escherichia coli. The substrate specificity of the recombinant Endo-LE was the same as that of the native enzyme, showing strong activity towards the highmannose type N-glycans with the Manβ1-2Manβ1- 3Manβ1- 4GlcNAcβ1-4GlcNAc unit. Real-time PCR analysis revealed that the gene expression of Endo-LE did not vary significantly with the tomato ripening process, indicating that Endo-LE activity is ubiquitously expressed.

AB - In this study, we identified a gene encoding tomato ENGase (Endo-LE) using the gene information of rice ENGase, and expressed the Endo-LE protein in Escherichia coli. The substrate specificity of the recombinant Endo-LE was the same as that of the native enzyme, showing strong activity towards the highmannose type N-glycans with the Manβ1-2Manβ1- 3Manβ1- 4GlcNAcβ1-4GlcNAc unit. Real-time PCR analysis revealed that the gene expression of Endo-LE did not vary significantly with the tomato ripening process, indicating that Endo-LE activity is ubiquitously expressed.

KW - De-N-glycosylation

KW - Endo-β-N-acetylglucosaminidase

KW - Free Nglycans

KW - Lycopersicon esculentu

KW - Tomato fruit ripening

UR - http://www.scopus.com/inward/record.url?scp=65249155427&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=65249155427&partnerID=8YFLogxK

U2 - 10.1271/bbb.80889

DO - 10.1271/bbb.80889

M3 - Article

C2 - 19202265

AN - SCOPUS:65249155427

VL - 73

SP - 461

EP - 464

JO - Bioscience, Biotechnology and Biochemistry

JF - Bioscience, Biotechnology and Biochemistry

SN - 0916-8451

IS - 2

ER -

Molecular cloning and gene expression analysis of tomato endo-β-n-acetylglucosaminidase, an endoglycosidase involved in the production of high-mannose Type Free N-Glycans during tomato fruit ripening

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this