Molecular cloning and gene expression analysis of tomato endo-β-n-acetylglucosaminidase, an endoglycosidase involved in the production of high-mannose Type Free N-Glycans during tomato fruit ripening

Kosuke Nakamura; Masami Inoue; Megumi Maeda; Ryohei Nakano; Katsutoshi Hosoi; Kazuhito Fujiyama; Yoshinobu Kimura

doi:10.1271/bbb.80889

Molecular cloning and gene expression analysis of tomato endo-β-n-acetylglucosaminidase, an endoglycosidase involved in the production of high-mannose Type Free N-Glycans during tomato fruit ripening

Kosuke Nakamura, Masami Inoue, Megumi Maeda, Ryohei Nakano, Katsutoshi Hosoi, Kazuhito Fujiyama, Yoshinobu Kimura

Research output: Contribution to journal › Article › peer-review

13 Citations (Scopus)

Abstract

In this study, we identified a gene encoding tomato ENGase (Endo-LE) using the gene information of rice ENGase, and expressed the Endo-LE protein in Escherichia coli. The substrate specificity of the recombinant Endo-LE was the same as that of the native enzyme, showing strong activity towards the highmannose type N-glycans with the Manβ1-2Manβ1- 3Manβ1- 4GlcNAcβ1-4GlcNAc unit. Real-time PCR analysis revealed that the gene expression of Endo-LE did not vary significantly with the tomato ripening process, indicating that Endo-LE activity is ubiquitously expressed.

Original language	English
Pages (from-to)	461-464
Number of pages	4
Journal	Bioscience, Biotechnology and Biochemistry
Volume	73
Issue number	2
DOIs	https://doi.org/10.1271/bbb.80889
Publication status	Published - Apr 30 2009

Keywords

De-N-glycosylation
Endo-β-N-acetylglucosaminidase
Free Nglycans
Lycopersicon esculentu
Tomato fruit ripening

ASJC Scopus subject areas

Biotechnology
Analytical Chemistry
Biochemistry
Applied Microbiology and Biotechnology
Molecular Biology
Organic Chemistry

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Nakamura, K., Inoue, M., Maeda, M., Nakano, R., Hosoi, K., Fujiyama, K., & Kimura, Y. (2009). Molecular cloning and gene expression analysis of tomato endo-β-n-acetylglucosaminidase, an endoglycosidase involved in the production of high-mannose Type Free N-Glycans during tomato fruit ripening. Bioscience, Biotechnology and Biochemistry, 73(2), 461-464. https://doi.org/10.1271/bbb.80889

Molecular cloning and gene expression analysis of tomato endo-β-n-acetylglucosaminidase, an endoglycosidase involved in the production of high-mannose Type Free N-Glycans during tomato fruit ripening. / Nakamura, Kosuke; Inoue, Masami; Maeda, Megumi; Nakano, Ryohei; Hosoi, Katsutoshi; Fujiyama, Kazuhito; Kimura, Yoshinobu.

In: Bioscience, Biotechnology and Biochemistry, Vol. 73, No. 2, 30.04.2009, p. 461-464.

Research output: Contribution to journal › Article › peer-review

Nakamura, K, Inoue, M, Maeda, M, Nakano, R, Hosoi, K, Fujiyama, K & Kimura, Y 2009, 'Molecular cloning and gene expression analysis of tomato endo-β-n-acetylglucosaminidase, an endoglycosidase involved in the production of high-mannose Type Free N-Glycans during tomato fruit ripening', Bioscience, Biotechnology and Biochemistry, vol. 73, no. 2, pp. 461-464. https://doi.org/10.1271/bbb.80889

Nakamura K, Inoue M, Maeda M, Nakano R, Hosoi K, Fujiyama K et al. Molecular cloning and gene expression analysis of tomato endo-β-n-acetylglucosaminidase, an endoglycosidase involved in the production of high-mannose Type Free N-Glycans during tomato fruit ripening. Bioscience, Biotechnology and Biochemistry. 2009 Apr 30;73(2):461-464. https://doi.org/10.1271/bbb.80889

Nakamura, Kosuke ; Inoue, Masami ; Maeda, Megumi ; Nakano, Ryohei ; Hosoi, Katsutoshi ; Fujiyama, Kazuhito ; Kimura, Yoshinobu. / Molecular cloning and gene expression analysis of tomato endo-β-n-acetylglucosaminidase, an endoglycosidase involved in the production of high-mannose Type Free N-Glycans during tomato fruit ripening. In: Bioscience, Biotechnology and Biochemistry. 2009 ; Vol. 73, No. 2. pp. 461-464.

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