Abstract
In this study, we identified a gene encoding tomato ENGase (Endo-LE) using the gene information of rice ENGase, and expressed the Endo-LE protein in Escherichia coli. The substrate specificity of the recombinant Endo-LE was the same as that of the native enzyme, showing strong activity towards the highmannose type N-glycans with the Manβ1-2Manβ1- 3Manβ1- 4GlcNAcβ1-4GlcNAc unit. Real-time PCR analysis revealed that the gene expression of Endo-LE did not vary significantly with the tomato ripening process, indicating that Endo-LE activity is ubiquitously expressed.
| Original language | English |
|---|---|
| Pages (from-to) | 461-464 |
| Number of pages | 4 |
| Journal | Bioscience, Biotechnology and Biochemistry |
| Volume | 73 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 2009 |
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Keywords
- De-N-glycosylation
- Endo-β-N-acetylglucosaminidase
- Free Nglycans
- Lycopersicon esculentu
- Tomato fruit ripening
ASJC Scopus subject areas
- Biotechnology
- Biochemistry
- Molecular Biology
- Applied Microbiology and Biotechnology
- Analytical Chemistry
- Organic Chemistry
- Medicine(all)
Cite this
Molecular cloning and gene expression analysis of tomato endo-β-n-acetylglucosaminidase, an endoglycosidase involved in the production of high-mannose Type Free N-Glycans during tomato fruit ripening. / Nakamura, Kosuke; Inoue, Masami; Maeda, Megumi; Nakano, Ryohei; Hosoi, Katsutoshi; Fujiyama, Kazuhito; Kimura, Yoshinobu.
In: Bioscience, Biotechnology and Biochemistry, Vol. 73, No. 2, 2009, p. 461-464.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Molecular cloning and gene expression analysis of tomato endo-β-n-acetylglucosaminidase, an endoglycosidase involved in the production of high-mannose Type Free N-Glycans during tomato fruit ripening
AU - Nakamura, Kosuke
AU - Inoue, Masami
AU - Maeda, Megumi
AU - Nakano, Ryohei
AU - Hosoi, Katsutoshi
AU - Fujiyama, Kazuhito
AU - Kimura, Yoshinobu
PY - 2009
Y1 - 2009
N2 - In this study, we identified a gene encoding tomato ENGase (Endo-LE) using the gene information of rice ENGase, and expressed the Endo-LE protein in Escherichia coli. The substrate specificity of the recombinant Endo-LE was the same as that of the native enzyme, showing strong activity towards the highmannose type N-glycans with the Manβ1-2Manβ1- 3Manβ1- 4GlcNAcβ1-4GlcNAc unit. Real-time PCR analysis revealed that the gene expression of Endo-LE did not vary significantly with the tomato ripening process, indicating that Endo-LE activity is ubiquitously expressed.
AB - In this study, we identified a gene encoding tomato ENGase (Endo-LE) using the gene information of rice ENGase, and expressed the Endo-LE protein in Escherichia coli. The substrate specificity of the recombinant Endo-LE was the same as that of the native enzyme, showing strong activity towards the highmannose type N-glycans with the Manβ1-2Manβ1- 3Manβ1- 4GlcNAcβ1-4GlcNAc unit. Real-time PCR analysis revealed that the gene expression of Endo-LE did not vary significantly with the tomato ripening process, indicating that Endo-LE activity is ubiquitously expressed.
KW - De-N-glycosylation
KW - Endo-β-N-acetylglucosaminidase
KW - Free Nglycans
KW - Lycopersicon esculentu
KW - Tomato fruit ripening
UR - http://www.scopus.com/inward/record.url?scp=65249155427&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=65249155427&partnerID=8YFLogxK
U2 - 10.1271/bbb.80889
DO - 10.1271/bbb.80889
M3 - Article
C2 - 19202265
AN - SCOPUS:65249155427
VL - 73
SP - 461
EP - 464
JO - Bioscience, Biotechnology and Biochemistry
JF - Bioscience, Biotechnology and Biochemistry
SN - 0916-8451
IS - 2
ER -