Molecular cloning and functional characterization of a vasotocin receptor subtype that is expressed in the shell gland and brain of the domestic chicken

Fen Lai Tan, Stephen J. Lolait, Michael J. Brownstein, Noboru Saito, Veronica Macleod, Dennis A. Baeyens, Philip R. Mayeux, Stacie M. Jones, Lawrence E. Cornett

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

In chickens, oviposition is correlated with increased plasma levels of the neurohypophysial hormone vasotocin, and vasotocin stimulates contraction of uterine strips in vitro. A gene encoding a vasotocin receptor subtype that we have designated the VT1 receptor was cloned from the domestic chicken. The open reading frame encodes a 370-amino acid polypeptide that displays seven segments of hydrophobic amino acids, typical of guanine nucleotide-protein- coupled receptors. Other structural features of the VT1 receptor include two potential N-linked glycosylation sites in the extracellular N-terminal region, a conserved aspartic acid in transmembrane domain 2 that is found in nearly all guanine nucleotide-protein-coupled receptors, and two potential protein kinase C phosphorylation sites in the third intracellular loop and C- terminal tail. Expressed VT1 receptors in COS7 cells bind neurohypophysial hormones with the following rank order of potency: vasotocin ≃ vasopressin > oxytocin ≃ mesotocin > isotocin. In addition, the expressed VT1 receptor mediates vasotocin-induced phosphatidylinositol turnover and Ca2+ mobilization. In the chicken, expression of VT1 receptor gene transcripts is limited to the shell gland (uterus) and the brain. Thus, the VT1 receptor that we have cloned may mediate contractions of the shell gland during oviposition and activate reproductive behaviors known to be stimulated by vasotocin in lower vertebrates.

Original languageEnglish
Pages (from-to)8-15
Number of pages8
JournalBiology of Reproduction
Volume62
Issue number1
Publication statusPublished - 2000
Externally publishedYes

Fingerprint

Molecular Cloning
Vasotocin
Chickens
Brain
Posterior Pituitary Hormones
Oviposition
Guanine Nucleotides
Amino Acids
Reproductive Behavior
Uterine Contraction
Oxytocin
Phosphatidylinositols
vasotocin receptor
Vasopressins
Glycosylation
Aspartic Acid
Protein Kinase C
Open Reading Frames
Genes
Uterus

ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology
  • Embryology

Cite this

Molecular cloning and functional characterization of a vasotocin receptor subtype that is expressed in the shell gland and brain of the domestic chicken. / Tan, Fen Lai; Lolait, Stephen J.; Brownstein, Michael J.; Saito, Noboru; Macleod, Veronica; Baeyens, Dennis A.; Mayeux, Philip R.; Jones, Stacie M.; Cornett, Lawrence E.

In: Biology of Reproduction, Vol. 62, No. 1, 2000, p. 8-15.

Research output: Contribution to journalArticle

Tan, FL, Lolait, SJ, Brownstein, MJ, Saito, N, Macleod, V, Baeyens, DA, Mayeux, PR, Jones, SM & Cornett, LE 2000, 'Molecular cloning and functional characterization of a vasotocin receptor subtype that is expressed in the shell gland and brain of the domestic chicken', Biology of Reproduction, vol. 62, no. 1, pp. 8-15.
Tan, Fen Lai ; Lolait, Stephen J. ; Brownstein, Michael J. ; Saito, Noboru ; Macleod, Veronica ; Baeyens, Dennis A. ; Mayeux, Philip R. ; Jones, Stacie M. ; Cornett, Lawrence E. / Molecular cloning and functional characterization of a vasotocin receptor subtype that is expressed in the shell gland and brain of the domestic chicken. In: Biology of Reproduction. 2000 ; Vol. 62, No. 1. pp. 8-15.
@article{a41669775b4f4bbdb64ce218dabdc71c,
title = "Molecular cloning and functional characterization of a vasotocin receptor subtype that is expressed in the shell gland and brain of the domestic chicken",
abstract = "In chickens, oviposition is correlated with increased plasma levels of the neurohypophysial hormone vasotocin, and vasotocin stimulates contraction of uterine strips in vitro. A gene encoding a vasotocin receptor subtype that we have designated the VT1 receptor was cloned from the domestic chicken. The open reading frame encodes a 370-amino acid polypeptide that displays seven segments of hydrophobic amino acids, typical of guanine nucleotide-protein- coupled receptors. Other structural features of the VT1 receptor include two potential N-linked glycosylation sites in the extracellular N-terminal region, a conserved aspartic acid in transmembrane domain 2 that is found in nearly all guanine nucleotide-protein-coupled receptors, and two potential protein kinase C phosphorylation sites in the third intracellular loop and C- terminal tail. Expressed VT1 receptors in COS7 cells bind neurohypophysial hormones with the following rank order of potency: vasotocin ≃ vasopressin > oxytocin ≃ mesotocin > isotocin. In addition, the expressed VT1 receptor mediates vasotocin-induced phosphatidylinositol turnover and Ca2+ mobilization. In the chicken, expression of VT1 receptor gene transcripts is limited to the shell gland (uterus) and the brain. Thus, the VT1 receptor that we have cloned may mediate contractions of the shell gland during oviposition and activate reproductive behaviors known to be stimulated by vasotocin in lower vertebrates.",
author = "Tan, {Fen Lai} and Lolait, {Stephen J.} and Brownstein, {Michael J.} and Noboru Saito and Veronica Macleod and Baeyens, {Dennis A.} and Mayeux, {Philip R.} and Jones, {Stacie M.} and Cornett, {Lawrence E.}",
year = "2000",
language = "English",
volume = "62",
pages = "8--15",
journal = "Biology of Reproduction",
issn = "0006-3363",
publisher = "Society for the Study of Reproduction",
number = "1",

}

TY - JOUR

T1 - Molecular cloning and functional characterization of a vasotocin receptor subtype that is expressed in the shell gland and brain of the domestic chicken

AU - Tan, Fen Lai

AU - Lolait, Stephen J.

AU - Brownstein, Michael J.

AU - Saito, Noboru

AU - Macleod, Veronica

AU - Baeyens, Dennis A.

AU - Mayeux, Philip R.

AU - Jones, Stacie M.

AU - Cornett, Lawrence E.

PY - 2000

Y1 - 2000

N2 - In chickens, oviposition is correlated with increased plasma levels of the neurohypophysial hormone vasotocin, and vasotocin stimulates contraction of uterine strips in vitro. A gene encoding a vasotocin receptor subtype that we have designated the VT1 receptor was cloned from the domestic chicken. The open reading frame encodes a 370-amino acid polypeptide that displays seven segments of hydrophobic amino acids, typical of guanine nucleotide-protein- coupled receptors. Other structural features of the VT1 receptor include two potential N-linked glycosylation sites in the extracellular N-terminal region, a conserved aspartic acid in transmembrane domain 2 that is found in nearly all guanine nucleotide-protein-coupled receptors, and two potential protein kinase C phosphorylation sites in the third intracellular loop and C- terminal tail. Expressed VT1 receptors in COS7 cells bind neurohypophysial hormones with the following rank order of potency: vasotocin ≃ vasopressin > oxytocin ≃ mesotocin > isotocin. In addition, the expressed VT1 receptor mediates vasotocin-induced phosphatidylinositol turnover and Ca2+ mobilization. In the chicken, expression of VT1 receptor gene transcripts is limited to the shell gland (uterus) and the brain. Thus, the VT1 receptor that we have cloned may mediate contractions of the shell gland during oviposition and activate reproductive behaviors known to be stimulated by vasotocin in lower vertebrates.

AB - In chickens, oviposition is correlated with increased plasma levels of the neurohypophysial hormone vasotocin, and vasotocin stimulates contraction of uterine strips in vitro. A gene encoding a vasotocin receptor subtype that we have designated the VT1 receptor was cloned from the domestic chicken. The open reading frame encodes a 370-amino acid polypeptide that displays seven segments of hydrophobic amino acids, typical of guanine nucleotide-protein- coupled receptors. Other structural features of the VT1 receptor include two potential N-linked glycosylation sites in the extracellular N-terminal region, a conserved aspartic acid in transmembrane domain 2 that is found in nearly all guanine nucleotide-protein-coupled receptors, and two potential protein kinase C phosphorylation sites in the third intracellular loop and C- terminal tail. Expressed VT1 receptors in COS7 cells bind neurohypophysial hormones with the following rank order of potency: vasotocin ≃ vasopressin > oxytocin ≃ mesotocin > isotocin. In addition, the expressed VT1 receptor mediates vasotocin-induced phosphatidylinositol turnover and Ca2+ mobilization. In the chicken, expression of VT1 receptor gene transcripts is limited to the shell gland (uterus) and the brain. Thus, the VT1 receptor that we have cloned may mediate contractions of the shell gland during oviposition and activate reproductive behaviors known to be stimulated by vasotocin in lower vertebrates.

UR - http://www.scopus.com/inward/record.url?scp=0033957711&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033957711&partnerID=8YFLogxK

M3 - Article

C2 - 10611061

AN - SCOPUS:0033957711

VL - 62

SP - 8

EP - 15

JO - Biology of Reproduction

JF - Biology of Reproduction

SN - 0006-3363

IS - 1

ER -