Molecular cloning and functional characterization of a prolactin-releasing peptide homolog from Xenopus laevis

Tatsuya Sakamoto, Aiko Oda, Kazutoshi Yamamoto, Miyoko Kaneko, Sakae Kikuyama, Akio Nishikawa, Akiyoshi Takahashi, Hiroshi Kawauchi, Kazuyoshi Tsutsui, Masaaki Fujimoto

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13 Citations (Scopus)

Abstract

Amino acid sequences for identified prolactin (PRL)-releasing peptides (PrRPs) were conserved in mammals (>90%) or teleost fishes (100%), but there were considerable differences between these classes in the sequence (<65%) as well as in the role of PrRP. In species other than fishes and mammals, we have identified frog PrRP. The cDNA encoding Xenopus laevis prepro-PrRP, which can generate putative PrRPs, was cloned and sequenced. Sequences for the coding region showed higher identity with teleost PrRPs than mammalian homologues, but suggested the occurrence of putative PrRPs of 20 and 31 residues as in mammals. The amino acid sequence of PrRP20 was only one residue different from teleost PrRP20, but shared 70% identity with mammalian PrRP20s. In primary cultures of bullfrog (Rana catesbeiana) pituitary cells, Xenopus PrRPs increased prolactin concentrations in culture medium to 130-160% of the control, but PrRPs was much less potent than thyrotropin-releasing hormone (TRH) causing a three- to four-fold increase in prolactin concentrations. PrRP mRNA levels in the developing Xenopus brain peak in early prometamorphosis, different from prolactin levels. PrRP may not be a major prolactin-releasing factor (PRF), at least in adult frogs, as in mammals.

Original languageEnglish
Pages (from-to)3347-3351
Number of pages5
JournalPeptides
Volume27
Issue number12
DOIs
Publication statusPublished - Dec 1 2006

Keywords

  • Amphibian
  • Brain
  • Evolution
  • Gene expression
  • Prolactin
  • Prolactin-releasing peptide

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Endocrinology
  • Cellular and Molecular Neuroscience

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  • Cite this

    Sakamoto, T., Oda, A., Yamamoto, K., Kaneko, M., Kikuyama, S., Nishikawa, A., Takahashi, A., Kawauchi, H., Tsutsui, K., & Fujimoto, M. (2006). Molecular cloning and functional characterization of a prolactin-releasing peptide homolog from Xenopus laevis. Peptides, 27(12), 3347-3351. https://doi.org/10.1016/j.peptides.2006.08.003