Molecular cloning and functional analysis of pea cDNA E86 encoding homologous protein to hypersensitivity-related hsr203J

Yuki Ichinose, Yumiko Hisayasu, Shiroh Sanematsu, Yasuhiro Ishiga, Hikaru Seki, Kazuhiro Toyoda, Tomonori Shiraishi, Tetsuji Yamada

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Clone E86 was isolated as cDNA for elicitor-inducible gene from pea epicotyls by differential screening. The deduced amino acid sequence of E86 showed high homology to hypersensitivity-related protein hsr203J in tobacco and also showed significant homologies to the Ser-active hydrolases, such as mammalian hormone-sensitive lipases, bacterial lipases and esterases. E86 polypeptide possesses consensus amino acid sequence motifs (His-Gly) and (Gly-X-Ser-X-Gly) conserved in lipases and esterases and showed esterase degradation of p-nitrophenyl butyrate. Northern blot analysis revealed that the E86-transcript is abundant in roots and stems and was induced by fungal elicitor in pea epicotyls. However, elicitor-induced accumulation of E86 mRNA was significantly inhibited by the fungal suppressor. Furthermore the expression of the genes encoding E86 and phenylalanine ammonia-lyase was induced within 1 h after the inoculation of a nonpathogen, but it was delayed for 5 h by the inoculation of a compatible pathogen. These results suggest that the elicitor-induced Ser-active hydrolase derived from E86 gene might be related to the plant defense responses.

Original languageEnglish
Pages (from-to)997-1006
Number of pages10
JournalPlant Science
Volume160
Issue number5
DOIs
Publication statusPublished - 2001

Fingerprint

Functional analysis
Cloning
Peas
Molecular Cloning
hypersensitive response
Esterases
molecular cloning
peas
Hypersensitivity
Complementary DNA
esterases
Hydrolases
Lipase
histidylglycine
epicotyls
Amino Acid Sequence
Genes
hydrolases
Phenylalanine Ammonia-Lyase
Sterol Esterase

Keywords

  • Elicitor
  • Esterase
  • Hydrolase
  • Lipase
  • Pea
  • Plant defense response

ASJC Scopus subject areas

  • Plant Science
  • Biochemistry
  • Biotechnology

Cite this

Molecular cloning and functional analysis of pea cDNA E86 encoding homologous protein to hypersensitivity-related hsr203J. / Ichinose, Yuki; Hisayasu, Yumiko; Sanematsu, Shiroh; Ishiga, Yasuhiro; Seki, Hikaru; Toyoda, Kazuhiro; Shiraishi, Tomonori; Yamada, Tetsuji.

In: Plant Science, Vol. 160, No. 5, 2001, p. 997-1006.

Research output: Contribution to journalArticle

Ichinose, Yuki ; Hisayasu, Yumiko ; Sanematsu, Shiroh ; Ishiga, Yasuhiro ; Seki, Hikaru ; Toyoda, Kazuhiro ; Shiraishi, Tomonori ; Yamada, Tetsuji. / Molecular cloning and functional analysis of pea cDNA E86 encoding homologous protein to hypersensitivity-related hsr203J. In: Plant Science. 2001 ; Vol. 160, No. 5. pp. 997-1006.
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