Abstract
A cDNA encoding a novel cytochrome P450 1A2 (CYP1A2) was cloned from the liver of an adult female Japanese monkey. The CYP1A2 protein was expressed in yeast cells and its enzymatic properties were compared with those of marmoset CYP1A2 using ethoxyresorufin (ER) and phenacetin (PN) as substrates. The nucleotide sequence of Japanese monkey CYP1A2 revealed 94.7, 99.5 and 93.5% identities to those of human, cynomolgus monkey and marmoset monkey CYP1A2, respectively. Multiple amino acid sequence alignment of Japanese monkey CYP1A2 with CYP1A2 of humans, cynomolgus monkeys and marmosets showed that Japanese monkey CYP1A2 had 92.4, 99.0 and 91.9% identities to the human, cynomolgus monkey and marmoset enzymes, respectively. Kinetic studies demonstrated that the enzymatic properties as ER and PN O-deethylases were considerably different between the Japanese monkey and the marmoset CYP1A2. Furthermore, both of these reactions in liver microsomal fractions from the Japanese monkey and marmoset showed biphasic kinetics. On the basis of the kinetic parameters, it is suggested that Japanese monkey CYP1A2 is a high-Km enzyme in both ER and PN O-deethylations, whereas marmoset CYP1A2 is a high-Km and low-Km enzyme in ER and PN O-deethylations, respectively. α-Naphthoflavone, an inhibitor of human CYP1A1 and CYP1A2, did not completely inhibit the liver microsomal oxidations of ER and PN even at the highest concentration (50 μM), supporting the notion that CYP1A2 enzymes are not the sole ER or PN O-deethylase in Japanese monkey and marmoset liver microsomes. Inhibitory effects of furafylline, an inhibitor of human CYP1A2, on ER O-deethylation by recombinant CYP1A2 enzymes were much lower than those of α-naphthoflavone, but marmoset CYP1A2 was more sensitive to furafylline than Japanese monkey CYP1A2. These results indicate that the properties of Japanese monkey CYP1A2 are considerably different from those of marmoset CYP1A2.
| Original language | English |
|---|---|
| Pages (from-to) | 1-12 |
| Number of pages | 12 |
| Journal | Chemico-Biological Interactions |
| Volume | 152 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Feb 28 2005 |
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Keywords
- Cytochrome P450 1A2
- Ethoxyresorufin
- Japanese monkey
- Marmoset
- Phenacetin
ASJC Scopus subject areas
- Toxicology
Cite this
Molecular cloning and functional analysis of cytochrome P450 1A2 from Japanese monkey liver : Comparison with marmoset cytochrome P450 1A2. / Narimatsu, Shizuo; Oda, Maiko; Hichiya, Hiroyuki; Isobe, Takashi; Asaoka, Kazuo; Hanioka, Nobumitsu; Yamano, Shigeru; Shinoda, Sumio; Yamamoto, Shigeo.
In: Chemico-Biological Interactions, Vol. 152, No. 1, 28.02.2005, p. 1-12.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Molecular cloning and functional analysis of cytochrome P450 1A2 from Japanese monkey liver
T2 - Comparison with marmoset cytochrome P450 1A2
AU - Narimatsu, Shizuo
AU - Oda, Maiko
AU - Hichiya, Hiroyuki
AU - Isobe, Takashi
AU - Asaoka, Kazuo
AU - Hanioka, Nobumitsu
AU - Yamano, Shigeru
AU - Shinoda, Sumio
AU - Yamamoto, Shigeo
PY - 2005/2/28
Y1 - 2005/2/28
N2 - A cDNA encoding a novel cytochrome P450 1A2 (CYP1A2) was cloned from the liver of an adult female Japanese monkey. The CYP1A2 protein was expressed in yeast cells and its enzymatic properties were compared with those of marmoset CYP1A2 using ethoxyresorufin (ER) and phenacetin (PN) as substrates. The nucleotide sequence of Japanese monkey CYP1A2 revealed 94.7, 99.5 and 93.5% identities to those of human, cynomolgus monkey and marmoset monkey CYP1A2, respectively. Multiple amino acid sequence alignment of Japanese monkey CYP1A2 with CYP1A2 of humans, cynomolgus monkeys and marmosets showed that Japanese monkey CYP1A2 had 92.4, 99.0 and 91.9% identities to the human, cynomolgus monkey and marmoset enzymes, respectively. Kinetic studies demonstrated that the enzymatic properties as ER and PN O-deethylases were considerably different between the Japanese monkey and the marmoset CYP1A2. Furthermore, both of these reactions in liver microsomal fractions from the Japanese monkey and marmoset showed biphasic kinetics. On the basis of the kinetic parameters, it is suggested that Japanese monkey CYP1A2 is a high-Km enzyme in both ER and PN O-deethylations, whereas marmoset CYP1A2 is a high-Km and low-Km enzyme in ER and PN O-deethylations, respectively. α-Naphthoflavone, an inhibitor of human CYP1A1 and CYP1A2, did not completely inhibit the liver microsomal oxidations of ER and PN even at the highest concentration (50 μM), supporting the notion that CYP1A2 enzymes are not the sole ER or PN O-deethylase in Japanese monkey and marmoset liver microsomes. Inhibitory effects of furafylline, an inhibitor of human CYP1A2, on ER O-deethylation by recombinant CYP1A2 enzymes were much lower than those of α-naphthoflavone, but marmoset CYP1A2 was more sensitive to furafylline than Japanese monkey CYP1A2. These results indicate that the properties of Japanese monkey CYP1A2 are considerably different from those of marmoset CYP1A2.
AB - A cDNA encoding a novel cytochrome P450 1A2 (CYP1A2) was cloned from the liver of an adult female Japanese monkey. The CYP1A2 protein was expressed in yeast cells and its enzymatic properties were compared with those of marmoset CYP1A2 using ethoxyresorufin (ER) and phenacetin (PN) as substrates. The nucleotide sequence of Japanese monkey CYP1A2 revealed 94.7, 99.5 and 93.5% identities to those of human, cynomolgus monkey and marmoset monkey CYP1A2, respectively. Multiple amino acid sequence alignment of Japanese monkey CYP1A2 with CYP1A2 of humans, cynomolgus monkeys and marmosets showed that Japanese monkey CYP1A2 had 92.4, 99.0 and 91.9% identities to the human, cynomolgus monkey and marmoset enzymes, respectively. Kinetic studies demonstrated that the enzymatic properties as ER and PN O-deethylases were considerably different between the Japanese monkey and the marmoset CYP1A2. Furthermore, both of these reactions in liver microsomal fractions from the Japanese monkey and marmoset showed biphasic kinetics. On the basis of the kinetic parameters, it is suggested that Japanese monkey CYP1A2 is a high-Km enzyme in both ER and PN O-deethylations, whereas marmoset CYP1A2 is a high-Km and low-Km enzyme in ER and PN O-deethylations, respectively. α-Naphthoflavone, an inhibitor of human CYP1A1 and CYP1A2, did not completely inhibit the liver microsomal oxidations of ER and PN even at the highest concentration (50 μM), supporting the notion that CYP1A2 enzymes are not the sole ER or PN O-deethylase in Japanese monkey and marmoset liver microsomes. Inhibitory effects of furafylline, an inhibitor of human CYP1A2, on ER O-deethylation by recombinant CYP1A2 enzymes were much lower than those of α-naphthoflavone, but marmoset CYP1A2 was more sensitive to furafylline than Japanese monkey CYP1A2. These results indicate that the properties of Japanese monkey CYP1A2 are considerably different from those of marmoset CYP1A2.
KW - Cytochrome P450 1A2
KW - Ethoxyresorufin
KW - Japanese monkey
KW - Marmoset
KW - Phenacetin
UR - http://www.scopus.com/inward/record.url?scp=17644412364&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=17644412364&partnerID=8YFLogxK
U2 - 10.1016/j.cbi.2005.01.006
DO - 10.1016/j.cbi.2005.01.006
M3 - Article
VL - 152
SP - 1
EP - 12
JO - Chemico-Biological Interactions
JF - Chemico-Biological Interactions
SN - 0009-2797
IS - 1
ER -