Molecular cloning and expression of three estrogen receptors in the protogynous wrasse, Halichoeres trimaculatus

Ryo Horiguchi, Yasuhisa Kobayashi, Masaru Nakamura

Research output: Contribution to journalArticle

Abstract

Estrogens are essential for sex change and behavior in wrasses. To understand the molecular mechanisms of estrogen action in the reproduction of the protogynous wrasse, Halichoeres trimaculatus, we isolated cDNA encoding three estrogen receptor (ER) subtypes from ovary by 5' and 3' rapid amplification cDNAends (RACE) methods. The deduced amino acid sequence of ERα,ERβ1 and ERβ2 showed high identity to the red seabream ERa (81%), largemouth bass ERβ (82%) and largemouth bass ERy (81%), respectively. Real-time polymerase chain reaction revealed some distinctions in tissue expression of ERs, suggesting different functions for each ER subtype in protogynous wrasses.

Original languageEnglish
Pages (from-to)88-89
Number of pages2
JournalCybium
Volume32
Issue number2 SUPPL.
Publication statusPublished - Jul 31 2008
Externally publishedYes

Fingerprint

Labridae
polymerase chain reaction
molecular cloning
amplification
amino acid
Micropterus salmoides
estrogens
Pagrus major
sex reversal
quantitative polymerase chain reaction
amino acid sequences
Halichoeres
estrogen receptors
tissue
cloning
method
methodology

Keywords

  • Cloning
  • Estrogen receptor
  • Expression
  • Halichoeres trimaculatus
  • Protogynous wrasse

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Ecology, Evolution, Behavior and Systematics

Cite this

Molecular cloning and expression of three estrogen receptors in the protogynous wrasse, Halichoeres trimaculatus. / Horiguchi, Ryo; Kobayashi, Yasuhisa; Nakamura, Masaru.

In: Cybium, Vol. 32, No. 2 SUPPL., 31.07.2008, p. 88-89.

Research output: Contribution to journalArticle

Horiguchi, R, Kobayashi, Y & Nakamura, M 2008, 'Molecular cloning and expression of three estrogen receptors in the protogynous wrasse, Halichoeres trimaculatus', Cybium, vol. 32, no. 2 SUPPL., pp. 88-89.
Horiguchi, Ryo ; Kobayashi, Yasuhisa ; Nakamura, Masaru. / Molecular cloning and expression of three estrogen receptors in the protogynous wrasse, Halichoeres trimaculatus. In: Cybium. 2008 ; Vol. 32, No. 2 SUPPL. pp. 88-89.
@article{4078388821314f25816172c398f2fd75,
title = "Molecular cloning and expression of three estrogen receptors in the protogynous wrasse, Halichoeres trimaculatus",
abstract = "Estrogens are essential for sex change and behavior in wrasses. To understand the molecular mechanisms of estrogen action in the reproduction of the protogynous wrasse, Halichoeres trimaculatus, we isolated cDNA encoding three estrogen receptor (ER) subtypes from ovary by 5' and 3' rapid amplification cDNAends (RACE) methods. The deduced amino acid sequence of ERα,ERβ1 and ERβ2 showed high identity to the red seabream ERa (81{\%}), largemouth bass ERβ (82{\%}) and largemouth bass ERy (81{\%}), respectively. Real-time polymerase chain reaction revealed some distinctions in tissue expression of ERs, suggesting different functions for each ER subtype in protogynous wrasses.",
keywords = "Cloning, Estrogen receptor, Expression, Halichoeres trimaculatus, Protogynous wrasse",
author = "Ryo Horiguchi and Yasuhisa Kobayashi and Masaru Nakamura",
year = "2008",
month = "7",
day = "31",
language = "English",
volume = "32",
pages = "88--89",
journal = "Cybium",
issn = "0399-0974",
publisher = "Societe Francaise d'Ichtyologie",
number = "2 SUPPL.",

}

TY - JOUR

T1 - Molecular cloning and expression of three estrogen receptors in the protogynous wrasse, Halichoeres trimaculatus

AU - Horiguchi, Ryo

AU - Kobayashi, Yasuhisa

AU - Nakamura, Masaru

PY - 2008/7/31

Y1 - 2008/7/31

N2 - Estrogens are essential for sex change and behavior in wrasses. To understand the molecular mechanisms of estrogen action in the reproduction of the protogynous wrasse, Halichoeres trimaculatus, we isolated cDNA encoding three estrogen receptor (ER) subtypes from ovary by 5' and 3' rapid amplification cDNAends (RACE) methods. The deduced amino acid sequence of ERα,ERβ1 and ERβ2 showed high identity to the red seabream ERa (81%), largemouth bass ERβ (82%) and largemouth bass ERy (81%), respectively. Real-time polymerase chain reaction revealed some distinctions in tissue expression of ERs, suggesting different functions for each ER subtype in protogynous wrasses.

AB - Estrogens are essential for sex change and behavior in wrasses. To understand the molecular mechanisms of estrogen action in the reproduction of the protogynous wrasse, Halichoeres trimaculatus, we isolated cDNA encoding three estrogen receptor (ER) subtypes from ovary by 5' and 3' rapid amplification cDNAends (RACE) methods. The deduced amino acid sequence of ERα,ERβ1 and ERβ2 showed high identity to the red seabream ERa (81%), largemouth bass ERβ (82%) and largemouth bass ERy (81%), respectively. Real-time polymerase chain reaction revealed some distinctions in tissue expression of ERs, suggesting different functions for each ER subtype in protogynous wrasses.

KW - Cloning

KW - Estrogen receptor

KW - Expression

KW - Halichoeres trimaculatus

KW - Protogynous wrasse

UR - http://www.scopus.com/inward/record.url?scp=77957603433&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77957603433&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:77957603433

VL - 32

SP - 88

EP - 89

JO - Cybium

JF - Cybium

SN - 0399-0974

IS - 2 SUPPL.

ER -