Molecular cloning and expression of three estrogen receptors in the protogynous wrasse, Halichoeres trimaculatus

Ryo Horiguchi, Yasuhisa Kobayashi, Masaru Nakamura

Research output: Contribution to journalArticle

Abstract

Estrogens are essential for sex change and behavior in wrasses. To understand the molecular mechanisms of estrogen action in the reproduction of the protogynous wrasse, Halichoeres trimaculatus, we isolated cDNA encoding three estrogen receptor (ER) subtypes from ovary by 5' and 3' rapid amplification cDNAends (RACE) methods. The deduced amino acid sequence of ERα,ERβ1 and ERβ2 showed high identity to the red seabream ERa (81%), largemouth bass ERβ (82%) and largemouth bass ERy (81%), respectively. Real-time polymerase chain reaction revealed some distinctions in tissue expression of ERs, suggesting different functions for each ER subtype in protogynous wrasses.

Original languageEnglish
Pages (from-to)88-89
Number of pages2
JournalCybium
Volume32
Issue number2 SUPPL.
Publication statusPublished - Jul 31 2008

Keywords

  • Cloning
  • Estrogen receptor
  • Expression
  • Halichoeres trimaculatus
  • Protogynous wrasse

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Animal Science and Zoology

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