Molecular cloning and expression of Thiobacillus ferrooxidans chromosomal ribulose bisphosphate carboxylase genes in Escherichia coli

Tomonobu Kusano, Kazuyuki Sugawara, Chihiro Inoue, Nobuhiro Suzuki

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The genes coding for d-ribulose-1,5-bisphosphate carboxylase (RuBPCase) from an iron-oxidizing bacterium, Thiobacillus ferrooxidans, were cloned into an Escherichia coli plasmid, pUC18. The recombinant plasmid, termed pTR11, contained a 4.0-kb PstI fragment including the entire coding regions for both large and small subunits of RuBPCase. Escherichia coli carrying pTR11 did not show any CO2-fixing activity. However, a derivative plasmid with an appropriate deletion, which was placed under the control of a tac promoter, conferred ribulose bisphosphate-dependent CO2-fixing activity on the host cell. Analysis of gel-filtration chromatography of the RuBPCase synthesized in E. coli revealed that it had a hexadecameric form like the native enzyme of T. ferrooxidans.

Original languageEnglish
Pages (from-to)35-41
Number of pages7
JournalCurrent Microbiology
Volume22
Issue number1
DOIs
Publication statusPublished - Jan 1 1991
Externally publishedYes

ASJC Scopus subject areas

  • Microbiology
  • Applied Microbiology and Biotechnology

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