Molecular cloning and expression of rat betacellulin cDNA

Hiroko Tada, Masaharu Seno, Hidenori Yamada, Reiko Sasada, Koichi Igarashi

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The cDNA encoding an entire open reading frame of rat betacellulin has been cloned from rat kidney. Expression of this cDNA in COS7 cells showed a significant amount of mitogenic activity in the culture media. Western blotting of the cell lysates suggested that the membrane-anchored precursor was cleaved to release its ectodomain very efficiently. Copyright (C) 2000.

Original languageEnglish
Pages (from-to)285-288
Number of pages4
JournalBBA - Gene Structure and Expression
Volume1492
Issue number1
DOIs
Publication statusPublished - Jun 21 2000

Fingerprint

Cloning
Molecular Cloning
Rats
Complementary DNA
Open Reading Frames
Culture Media
Western Blotting
Membranes
Kidney
Betacellulin

Keywords

  • Betacellulin
  • Epidermal growth factor family
  • Membrane-anchored growth factor
  • Pancreatic cell differentiation
  • Secretase

ASJC Scopus subject areas

  • Biochemistry
  • Genetics
  • Structural Biology
  • Biophysics

Cite this

Molecular cloning and expression of rat betacellulin cDNA. / Tada, Hiroko; Seno, Masaharu; Yamada, Hidenori; Sasada, Reiko; Igarashi, Koichi.

In: BBA - Gene Structure and Expression, Vol. 1492, No. 1, 21.06.2000, p. 285-288.

Research output: Contribution to journalArticle

Tada, Hiroko ; Seno, Masaharu ; Yamada, Hidenori ; Sasada, Reiko ; Igarashi, Koichi. / Molecular cloning and expression of rat betacellulin cDNA. In: BBA - Gene Structure and Expression. 2000 ; Vol. 1492, No. 1. pp. 285-288.
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