Molecular cloning and expression of rat betacellulin cDNA

Hiroko Tada; Masaharu Seno; Hidenori Yamada; Reiko Sasada; Koichi Igarashi

doi:10.1016/S0167-4781(00)00106-8

Molecular cloning and expression of rat betacellulin cDNA

Hiroko Tada, Masaharu Seno, Hidenori Yamada, Reiko Sasada, Koichi Igarashi

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

The cDNA encoding an entire open reading frame of rat betacellulin has been cloned from rat kidney. Expression of this cDNA in COS7 cells showed a significant amount of mitogenic activity in the culture media. Western blotting of the cell lysates suggested that the membrane-anchored precursor was cleaved to release its ectodomain very efficiently. Copyright (C) 2000.

Original language	English
Pages (from-to)	285-288
Number of pages	4
Journal	Biochimica et Biophysica Acta - Gene Structure and Expression
Volume	1492
Issue number	1
DOIs	https://doi.org/10.1016/S0167-4781(00)00106-8
Publication status	Published - Jun 21 2000

Keywords

Betacellulin
Epidermal growth factor family
Membrane-anchored growth factor
Pancreatic cell differentiation
Secretase

ASJC Scopus subject areas

Structural Biology
Biophysics
Biochemistry
Genetics

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title = "Molecular cloning and expression of rat betacellulin cDNA",

abstract = "The cDNA encoding an entire open reading frame of rat betacellulin has been cloned from rat kidney. Expression of this cDNA in COS7 cells showed a significant amount of mitogenic activity in the culture media. Western blotting of the cell lysates suggested that the membrane-anchored precursor was cleaved to release its ectodomain very efficiently. Copyright (C) 2000.",

keywords = "Betacellulin, Epidermal growth factor family, Membrane-anchored growth factor, Pancreatic cell differentiation, Secretase",

author = "Hiroko Tada and Masaharu Seno and Hidenori Yamada and Reiko Sasada and Koichi Igarashi",

note = "Funding Information: The authors thank Ms. M. Kosaka for her assistance. The present study was supported in part by a Grant-in-Aid for Okayama Foundation for Science and Technology. Copyright: Copyright 2007 Elsevier B.V., All rights reserved.",

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T1 - Molecular cloning and expression of rat betacellulin cDNA

AU - Tada, Hiroko

AU - Seno, Masaharu

AU - Yamada, Hidenori

AU - Sasada, Reiko

AU - Igarashi, Koichi

N1 - Funding Information: The authors thank Ms. M. Kosaka for her assistance. The present study was supported in part by a Grant-in-Aid for Okayama Foundation for Science and Technology. Copyright: Copyright 2007 Elsevier B.V., All rights reserved.

PY - 2000/6/21

Y1 - 2000/6/21

N2 - The cDNA encoding an entire open reading frame of rat betacellulin has been cloned from rat kidney. Expression of this cDNA in COS7 cells showed a significant amount of mitogenic activity in the culture media. Western blotting of the cell lysates suggested that the membrane-anchored precursor was cleaved to release its ectodomain very efficiently. Copyright (C) 2000.

AB - The cDNA encoding an entire open reading frame of rat betacellulin has been cloned from rat kidney. Expression of this cDNA in COS7 cells showed a significant amount of mitogenic activity in the culture media. Western blotting of the cell lysates suggested that the membrane-anchored precursor was cleaved to release its ectodomain very efficiently. Copyright (C) 2000.

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KW - Epidermal growth factor family

KW - Membrane-anchored growth factor

KW - Pancreatic cell differentiation

KW - Secretase

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VL - 1492

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JO - Biochimica et Biophysica Acta - Gene Structure and Expression

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Molecular cloning and expression of rat betacellulin cDNA

Abstract

Keywords

ASJC Scopus subject areas

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