Abstract
The cDNA encoding an entire open reading frame of rat betacellulin has been cloned from rat kidney. Expression of this cDNA in COS7 cells showed a significant amount of mitogenic activity in the culture media. Western blotting of the cell lysates suggested that the membrane-anchored precursor was cleaved to release its ectodomain very efficiently. Copyright (C) 2000.
| Original language | English |
|---|---|
| Pages (from-to) | 285-288 |
| Number of pages | 4 |
| Journal | BBA - Gene Structure and Expression |
| Volume | 1492 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Jun 21 2000 |
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Keywords
- Betacellulin
- Epidermal growth factor family
- Membrane-anchored growth factor
- Pancreatic cell differentiation
- Secretase
ASJC Scopus subject areas
- Biochemistry
- Genetics
- Structural Biology
- Biophysics
Cite this
Molecular cloning and expression of rat betacellulin cDNA. / Tada, Hiroko; Seno, Masaharu; Yamada, Hidenori; Sasada, Reiko; Igarashi, Koichi.
In: BBA - Gene Structure and Expression, Vol. 1492, No. 1, 21.06.2000, p. 285-288.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Molecular cloning and expression of rat betacellulin cDNA
AU - Tada, Hiroko
AU - Seno, Masaharu
AU - Yamada, Hidenori
AU - Sasada, Reiko
AU - Igarashi, Koichi
PY - 2000/6/21
Y1 - 2000/6/21
N2 - The cDNA encoding an entire open reading frame of rat betacellulin has been cloned from rat kidney. Expression of this cDNA in COS7 cells showed a significant amount of mitogenic activity in the culture media. Western blotting of the cell lysates suggested that the membrane-anchored precursor was cleaved to release its ectodomain very efficiently. Copyright (C) 2000.
AB - The cDNA encoding an entire open reading frame of rat betacellulin has been cloned from rat kidney. Expression of this cDNA in COS7 cells showed a significant amount of mitogenic activity in the culture media. Western blotting of the cell lysates suggested that the membrane-anchored precursor was cleaved to release its ectodomain very efficiently. Copyright (C) 2000.
KW - Betacellulin
KW - Epidermal growth factor family
KW - Membrane-anchored growth factor
KW - Pancreatic cell differentiation
KW - Secretase
UR - http://www.scopus.com/inward/record.url?scp=0034697862&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0034697862&partnerID=8YFLogxK
U2 - 10.1016/S0167-4781(00)00106-8
DO - 10.1016/S0167-4781(00)00106-8
M3 - Article
C2 - 11004502
AN - SCOPUS:0034697862
VL - 1492
SP - 285
EP - 288
JO - Biochimica et Biophysica Acta - Gene Structure and Expression
JF - Biochimica et Biophysica Acta - Gene Structure and Expression
SN - 0167-4781
IS - 1
ER -