Molecular cloning and expression of human ribonuclease 4 cDNA

Masaharu Seno, Jun ichiro Futami, Yoshiaki Tsushima, Kazumi Akutagawa, Megumi Kosaka, Hiroko Tada, Hidenori Yamada

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

A cDNA coding for human ribonuclease 4 was isolated from a pancreas cDNA library and sequenced. This cDNA (996 bp) includes an entire open reading frame encoding mature protein (119 aa) following signal peptide (28 aa). Expression of mature protein in Escherichia coli showed an apparent molecular mass of about 16 kDa, which was slightly lower than the mature form of human RNase 1, in SDS-PAGE.

Original languageEnglish
Pages (from-to)424-426
Number of pages3
JournalBBA - Gene Structure and Expression
Volume1261
Issue number3
DOIs
Publication statusPublished - Apr 26 1995

Keywords

  • (Human)
  • (Pancreas)
  • Gene expression
  • Pancreatic type ribonuclease family
  • RNase 4
  • cDNA cloning

ASJC Scopus subject areas

  • Structural Biology
  • Biophysics
  • Biochemistry
  • Genetics

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