Molecular cloning and expression of farnesyl pyrophosphate synthase gene responsible for essential oil biosynthesis in hop (Humulus lupulus)

Yukio Okada, Manabu Sugimoto, Kazutoshi Ito

    Research output: Contribution to journalArticlepeer-review

    7 Citations (Scopus)

    Abstract

    Farnesyl pyrophosphate (FPP) synthase catalyzes the biosynthesis of geranyl pyrophosphate (GPP) and FPP, which are the precursors of terpenoids such as essential oils, from isopentenyl pyrophosphate (IPP) and dimethylallyl pyrophosphate (DMAPP). gDNA and cDNA encoding hop (Humulus lupulus L.) FPP synthase was cloned by the Inverse PCR method and the Cassette-mediated PCR method and sequenced. The deduced amino acid sequence showed a high identity to plant FPP synthases. The cloned gene was expressed in Escherichia coli cells to produce the FPP synthase. Northern blot analysis revealed that the FPP synthase gene was expressed in the lupulin gland, bract, leaf, and stem of hop and expressed more strongly in the lupulin gland than in other tissues.

    Original languageEnglish
    Pages (from-to)1183-1188
    Number of pages6
    JournalJournal of Plant Physiology
    Volume158
    Issue number9
    DOIs
    Publication statusPublished - Jan 1 2001

    Keywords

    • Essential oil
    • Farnesyl pyrophosphate synthase
    • Hop
    • Humulus lupulus
    • Lupulin gland
    • Prenyltransferase

    ASJC Scopus subject areas

    • Physiology
    • Agronomy and Crop Science
    • Plant Science

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