Molecular cloning and expression of farnesyl pyrophosphate synthase gene responsible for essential oil biosynthesis in hop (Humulus lupulus)

Yukio Okada, Manabu Sugimoto, Kazutoshi Ito

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Farnesyl pyrophosphate (FPP) synthase catalyzes the biosynthesis of geranyl pyrophosphate (GPP) and FPP, which are the precursors of terpenoids such as essential oils, from isopentenyl pyrophosphate (IPP) and dimethylallyl pyrophosphate (DMAPP). gDNA and cDNA encoding hop (Humulus lupulus L.) FPP synthase was cloned by the Inverse PCR method and the Cassette-mediated PCR method and sequenced. The deduced amino acid sequence showed a high identity to plant FPP synthases. The cloned gene was expressed in Escherichia coli cells to produce the FPP synthase. Northern blot analysis revealed that the FPP synthase gene was expressed in the lupulin gland, bract, leaf, and stem of hop and expressed more strongly in the lupulin gland than in other tissues.

Original languageEnglish
Pages (from-to)1183-1188
Number of pages6
JournalJournal of Plant Physiology
Volume158
Issue number9
Publication statusPublished - 2001

Fingerprint

Humulus lupulus
Humulus
hops
pyrophosphates
Molecular Cloning
Volatile Oils
molecular cloning
essential oils
biosynthesis
Genes
genes
Polymerase Chain Reaction
Terpenes
Northern Blotting
farnesyl pyrophosphate
Amino Acid Sequence
Complementary DNA
bracts
Escherichia coli
Northern blotting

Keywords

  • Essential oil
  • Farnesyl pyrophosphate synthase
  • Hop
  • Humulus lupulus
  • Lupulin gland
  • Prenyltransferase

ASJC Scopus subject areas

  • Plant Science

Cite this

Molecular cloning and expression of farnesyl pyrophosphate synthase gene responsible for essential oil biosynthesis in hop (Humulus lupulus). / Okada, Yukio; Sugimoto, Manabu; Ito, Kazutoshi.

In: Journal of Plant Physiology, Vol. 158, No. 9, 2001, p. 1183-1188.

Research output: Contribution to journalArticle

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