TY - JOUR
T1 - Molecular cloning and expression of Ad4BP/SF-1 in the serial sex changing gobiid fish, Trimma okinawae
AU - Kobayashi, Yasuhisa
AU - Sunobe, Tomoki
AU - Kobayashi, Tohru
AU - Nakamura, Masaru
AU - Suzuki, Norio
AU - Nagahama, Yoshitaka
N1 - Funding Information:
We thank Dr. Bindu Paul (National Institute for Basic Biology) and Dr. Francesc Piferrer (Consejo Superior de Investigaciones Cientificas) for critical reading of the manuscript. This work has been supported by CREST of JST (Japan Science and Technology Corporation), Grants in-Aid from the Ministry of Education, Culture, Sports, Science and Technology and Takeda science foundation, Bio-Design Program from Ministry of Agriculture, Forestry, and Fisheries, Japan, a Grant-in-Aid for Scientific Research (C16570025) from Ministry of Education, Culture, Sports, Science and Technology, Japan, and 21st Century COE program of the University of the Ryukyus. This is the contribution from Sesoko Station, Tropical Biosphere Research Center, University of the Ryukyus.
PY - 2005/7/15
Y1 - 2005/7/15
N2 - Involvement of Ad4BP/SF-1 was investigated using the serial sex changing goby Trimma okinawae. First, a cDNA encoding Ad4BP/SF-1 was cloned from ovarian follicles. The open reading frame of goby Ad4BP/SF-1 encodes a protein of 489 amino acids. Ad4BP/SF-1 was expressed in gonadal tissues, brain, and kidney. Second, transcript levels of Ad4BP/SF-1 were measured in the ovary and testis of the same individuals. Expressions were related to sexual phases. Moreover, ovarian expression of Ad4BP/SF-1 increased during vitellogenesis and declined sharply during the post-vitellogenic period. Finally, the expression profile of Ad4BP/SF-1 was measured during serial sex change in the ovary. Ad4BP/SF-1 increased in parallel with the onset of the female-phase and decreased as female became male. Therefore, Ad4BP/SF-1 probably acts as an important indirect regulator of oocyte growth and maturation at female-phase of serial sex changing gobiid fish T. okinawae.
AB - Involvement of Ad4BP/SF-1 was investigated using the serial sex changing goby Trimma okinawae. First, a cDNA encoding Ad4BP/SF-1 was cloned from ovarian follicles. The open reading frame of goby Ad4BP/SF-1 encodes a protein of 489 amino acids. Ad4BP/SF-1 was expressed in gonadal tissues, brain, and kidney. Second, transcript levels of Ad4BP/SF-1 were measured in the ovary and testis of the same individuals. Expressions were related to sexual phases. Moreover, ovarian expression of Ad4BP/SF-1 increased during vitellogenesis and declined sharply during the post-vitellogenic period. Finally, the expression profile of Ad4BP/SF-1 was measured during serial sex change in the ovary. Ad4BP/SF-1 increased in parallel with the onset of the female-phase and decreased as female became male. Therefore, Ad4BP/SF-1 probably acts as an important indirect regulator of oocyte growth and maturation at female-phase of serial sex changing gobiid fish T. okinawae.
KW - Ad4BP/SF-1
KW - Gobiid fish
KW - Gonadal development and maturation
KW - Sex changing fish
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U2 - 10.1016/j.bbrc.2005.05.062
DO - 10.1016/j.bbrc.2005.05.062
M3 - Article
C2 - 15925324
AN - SCOPUS:20444371926
VL - 332
SP - 1073
EP - 1080
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 4
ER -