Molecular cloning and expression of a gene for a factor which stabilizes formation of inhibitor-mitochondrial Atpase complex from Saccharomyces cerevisiae

Akira Akashi, Yukuo Yoshida, Hideki Nakagoshi, Kazuyuki Kuroki, Tadao Hashimoto, Kunio Tagawa, Fumio Imamoto

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Stabilizing factor, a 9 kDa protein, stabilizes and facilitates formation of the complex between mitochondrial ATP synthase and its intrinsic inhibitor protein. A clone containing the gene encoding the 9 kDa protein was selected from a yeast genomic library to determine the structure of its precursor protein. As deduced from the nucleotide sequence, the precursor of the yeast 9 kDa stabilizing factor contains 86 amino acid residues and has a molecular weight of 10,062. From the predicted sequence we infer that the stabilizing factor precursor contains a presequence of 23 amino acid residues at its amino terminus. We also used S1 mapping to determine the initiation site of transcription under glucose-repressed or derepressed conditions. These experiments suggest that transcription of this gene starts at three different sites and that only one of them is not affected by the presence of glucose.

Original languageEnglish
Pages (from-to)526-530
Number of pages5
JournalJournal of Biochemistry
Volume104
Issue number4
Publication statusPublished - Oct 1988
Externally publishedYes

Fingerprint

Cloning
ATP Synthase
Molecular Cloning
Saccharomyces Cerevisiae
Yeast
Inhibitor
Saccharomyces cerevisiae
Adenosine Triphosphatases
Genes
Precursor
Gene
Proteins
Protein
Gene Expression
Transcription
Yeasts
Mitochondrial Proton-Translocating ATPases
Glucose
Amino Acids
Amino acids

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Physiology (medical)
  • Radiology Nuclear Medicine and imaging
  • Molecular Biology
  • Biochemistry

Cite this

Molecular cloning and expression of a gene for a factor which stabilizes formation of inhibitor-mitochondrial Atpase complex from Saccharomyces cerevisiae. / Akashi, Akira; Yoshida, Yukuo; Nakagoshi, Hideki; Kuroki, Kazuyuki; Hashimoto, Tadao; Tagawa, Kunio; Imamoto, Fumio.

In: Journal of Biochemistry, Vol. 104, No. 4, 10.1988, p. 526-530.

Research output: Contribution to journalArticle

Akashi, Akira ; Yoshida, Yukuo ; Nakagoshi, Hideki ; Kuroki, Kazuyuki ; Hashimoto, Tadao ; Tagawa, Kunio ; Imamoto, Fumio. / Molecular cloning and expression of a gene for a factor which stabilizes formation of inhibitor-mitochondrial Atpase complex from Saccharomyces cerevisiae. In: Journal of Biochemistry. 1988 ; Vol. 104, No. 4. pp. 526-530.
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