Molecular cloning and cold-inducible gene expression of ferritin H subunit isoforms in rainbow trout cells

Michiaki Yamashita, Nobuhiko Ojima, Tatsuya Sakamoto

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Cold acclimation of rainbow trout cells is considered to be mediated by alterations in the mRNAs and proteins present in cold-treated cells. A subtracted cDNA library from cold-treated rainbow trout RTG-2 cells was constructed and screened to isolate cDNA induced in the cold-treated cells in order to elucidate which genes are induced by cold acclimation. A set of cDNA clones encoding three members of ferritin H isoforms was isolated as cold- inducible genes. Northern blot analysis and nuclear run-on transcription assay showed that the transcription and accumulation of the ferritin H isoforms mRNA were enhanced by cold acclimation. Furthermore, the ferritin level in the trout cells increased on cold acclimation in response to a temperature shift from 22 °C to 4 °C. When the trout cells were subjected to 4 °C under the condition of a decreased ferritin H level obtained by the addition of an antisense oligonucleotide, cell growth was apparently inhibited. These findings indicate an association between the induction of ferritin H and cellular mechanisms during cold acclimation of trout cells.

Original languageEnglish
Pages (from-to)26908-26913
Number of pages6
JournalJournal of Biological Chemistry
Volume271
Issue number43
DOIs
Publication statusPublished - 1996
Externally publishedYes

Fingerprint

Apoferritins
Cloning
Oncorhynchus mykiss
Molecular Cloning
Gene expression
Protein Isoforms
Gene Expression
Acclimatization
Transcription
Complementary DNA
Trout
Genes
Cells
RNA Isoforms
Antisense Oligonucleotides
Cell growth
Ferritins
Gene Library
Assays
Association reactions

ASJC Scopus subject areas

  • Biochemistry

Cite this

Molecular cloning and cold-inducible gene expression of ferritin H subunit isoforms in rainbow trout cells. / Yamashita, Michiaki; Ojima, Nobuhiko; Sakamoto, Tatsuya.

In: Journal of Biological Chemistry, Vol. 271, No. 43, 1996, p. 26908-26913.

Research output: Contribution to journalArticle

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