Molecular cloning and cold-inducible gene expression of ferritin H subunit isoforms in rainbow trout cells

Michiaki Yamashita, Nobuhiko Ojima, Tatsuya Sakamoto

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Cold acclimation of rainbow trout cells is considered to be mediated by alterations in the mRNAs and proteins present in cold-treated cells. A subtracted cDNA library from cold-treated rainbow trout RTG-2 cells was constructed and screened to isolate cDNA induced in the cold-treated cells in order to elucidate which genes are induced by cold acclimation. A set of cDNA clones encoding three members of ferritin H isoforms was isolated as cold- inducible genes. Northern blot analysis and nuclear run-on transcription assay showed that the transcription and accumulation of the ferritin H isoforms mRNA were enhanced by cold acclimation. Furthermore, the ferritin level in the trout cells increased on cold acclimation in response to a temperature shift from 22 °C to 4 °C. When the trout cells were subjected to 4 °C under the condition of a decreased ferritin H level obtained by the addition of an antisense oligonucleotide, cell growth was apparently inhibited. These findings indicate an association between the induction of ferritin H and cellular mechanisms during cold acclimation of trout cells.

Original languageEnglish
Pages (from-to)26908-26913
Number of pages6
JournalJournal of Biological Chemistry
Volume271
Issue number43
DOIs
Publication statusPublished - Nov 6 1996
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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