Molecular cloning and characterization of a steroid receptor-binding regulator of G-protein signaling protein cDNA

Mitsunori Ikeda, Mariko Hirokawa, Nahoko Satani, Tomomi Kinoshita, Yoshimi Watanabe, Hiroko Inoue, Shigenobu Tone, Tetsuya Ishikawa, Yohsuke Minatogawa

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)

Abstract

Steroid hormone receptors are composed of six major functional domains, i.e. the A/B domains as the activation function 1 domain (AF-1), domain C as the DNA-binding domain, domain D as a hinge domain and domain E/F as the ligand-dependent transcriptional domain (AF-2). They regulate gene transcription through interactions with various nuclear factors of their domains, such as AF-1 and AF-2. We have insufficient knowledge of the function of the DNA-binding domain (domain C) except for its DNA-binding function or the hinge domain (domain D). Therefore, we attempted to identify factors interacting with the domains by using a yeast two-hybrid system. Domains C and D of estrogen receptor α were used as a bait to isolate cDNA clones from a rat ovary cDNA library. We isolated the cDNA clone of a novel steroid receptor-binding protein bearing the regulator of G-protein signaling (RGS) designated as SRB-RGS. The protein repressed the transcriptional activity of estrogen receptor α, suggesting cross-talk of steroid hormones and peptide hormones (or growth factors) for signal transductions mediated by SRB-RGS.

Original languageEnglish
Pages (from-to)207-214
Number of pages8
JournalGene
Volume273
Issue number2
DOIs
Publication statusPublished - Aug 8 2001
Externally publishedYes

Keywords

  • Cross-talk
  • Estrogen receptor
  • PDZ domain
  • Signal transduction
  • Transcription

ASJC Scopus subject areas

  • Genetics

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