Molecular cloning and characterization of a cDNA for a novel phorbol-12-myristate-13-acetate-responsive gene that is highly expressed in an adult T-cell leukemia cell line

Makoto Hijikata, Nobuyuki Kato, Takako Sato, Yoshitoyo Kagami, Kunitada Shimotohno

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

To identify gene products that might be involved in leukemogenesis of adult T-cell leukemia (ATL), we constructed a cDNA library from an ATL tumor cell line named IKD. By differential plaque hybridization using [32P]cUNAs of poly(A)+ RNA from IKD cells and a human T-lymphotropic virus type I-infected T-cell line (C91/PL) as probes and RNA blot analysis, we obtained a single cDNA clone of a gene that is highly expressed in IKD cells. Expression of this gene was also detected in fresh peripheral blood mononuclear cells of several ATL patients but not in those of healthy donors. Sequence analysis showed that the cDNA was that of a previously undescribed gene. On structural analysis of the cDNA (1,897 base pairs), a short open reading frame encoding a polypeptide of 54 amino acid residues was found. Exposure of human peripheral blood mononuclear cells, a T-cell lymphoma cell line (Jurkat), and quiescent human embryonic lung cells to phorbol-12-myristate-13-acetate resulted in rapid, transient expression of 2.0-kilobase mRNA of this gene. This induction of the gene was not inhibited by an inhibitor of protein synthesis, cycloheximide. From these findings, we suggest that this gene, named APR, is a member of the cellular immediate-early-response genes.

Original languageEnglish
Pages (from-to)4632-4639
Number of pages8
JournalJournal of Virology
Volume64
Issue number10
Publication statusPublished - 1990
Externally publishedYes

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Adult T Cell Leukemia Lymphoma
Molecular Cloning
leukemia
molecular cloning
Acetates
Complementary DNA
T-lymphocytes
acetates
cell lines
Cell Line
Genes
genes
mononuclear leukocytes
Blood Cells
RNA Probes
protein synthesis inhibitors
Messenger RNA
Protein Synthesis Inhibitors
Immediate-Early Genes
T-Cell Lymphoma

ASJC Scopus subject areas

  • Immunology

Cite this

Molecular cloning and characterization of a cDNA for a novel phorbol-12-myristate-13-acetate-responsive gene that is highly expressed in an adult T-cell leukemia cell line. / Hijikata, Makoto; Kato, Nobuyuki; Sato, Takako; Kagami, Yoshitoyo; Shimotohno, Kunitada.

In: Journal of Virology, Vol. 64, No. 10, 1990, p. 4632-4639.

Research output: Contribution to journalArticle

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