TY - JOUR
T1 - Molecular characterization of the hlyX-like gene of actinobacillus actinomycetemcomitans Y4
AU - Kokeguchi, Susumu
AU - Hirosue, Masaru
AU - Maeda, Hiroshi
AU - Miyamoto, Manabu
AU - Takashiba, Shogo
AU - Murayama, Yoji
N1 - Funding Information:
We thank Dr J.I. MacIness (University of Guelph, Ontario, Canada) and Dr J.R. Guest (University of Sheffield, Sheffield, UK) for providing the plasmid pT51 and E. coli strain JRG1728, respectively. We also thank Dr Satoko Sawada for assistance with computer analysis. This study was supported by grants-in-aid for scientific research (number 11672082 and 11897023 ) from the Ministry of Education, Science, Sports and Culture of Japan.
PY - 2000
Y1 - 2000
N2 - We isolated and characterized a possible regulatory gene, designated actX gene, from Actinobacillus actinomyctemcomitans Y4, which defined the Actinobacillus pleuropneumoniae hlyX-like regulatory gene. DNA sequence analysis for plasmid clone pKM317 containing a 1.6-kb DNA insert indicated an open reading frame encoding a polypeptide of 257 amino acid residues. Analysis of the deduced amino acid sequence showed the presence of five characteristic cysteine residues in the N-terminal region and a putative DNA binding residue in the C-terminal region, indicating that actX might belong to a regulatory gene family. Escherichia coli DH5α and a mutant strain JRG1728 transformed by plasmid carrying actX manifested apparent hemolytic activity on sheep blood agar and grew anaerobically, although the original strains did not. (C) 2000 Editions scientifiques et medicales Elsevier SAS.
AB - We isolated and characterized a possible regulatory gene, designated actX gene, from Actinobacillus actinomyctemcomitans Y4, which defined the Actinobacillus pleuropneumoniae hlyX-like regulatory gene. DNA sequence analysis for plasmid clone pKM317 containing a 1.6-kb DNA insert indicated an open reading frame encoding a polypeptide of 257 amino acid residues. Analysis of the deduced amino acid sequence showed the presence of five characteristic cysteine residues in the N-terminal region and a putative DNA binding residue in the C-terminal region, indicating that actX might belong to a regulatory gene family. Escherichia coli DH5α and a mutant strain JRG1728 transformed by plasmid carrying actX manifested apparent hemolytic activity on sheep blood agar and grew anaerobically, although the original strains did not. (C) 2000 Editions scientifiques et medicales Elsevier SAS.
KW - Actinobacillus actinomycetemcomitans
KW - HlyX-like gene
KW - Regulatory gene
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U2 - 10.1016/S0923-2508(00)01137-2
DO - 10.1016/S0923-2508(00)01137-2
M3 - Article
C2 - 11130862
AN - SCOPUS:0033694548
SN - 0923-2508
VL - 151
SP - 721
EP - 725
JO - Research in Microbiology
JF - Research in Microbiology
IS - 9
ER -