Molecular characterization of plant acidic α-mannosidase, a member of glycosylhydrolase family 38, involved in the turnover of N-glycans during tomato fruit ripening

Md Anowar Hossain, Ryohei Nakano, Kosuke Nakamura, Md Tofazzal Hossain, Yoshinobu Kimura

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

It has been reported that acidic α-mannosidase activity increases during tomato fruit ripening, suggesting the turnover of N-glycoproteins is deeply associated with fruit ripening. As part of a study to reveal the relationship between the plant α-mannosidase activity and fruit maturation at the molecular level, we have already purified and characterized an α-mannosidase from tomato fruit (Hossain et al., Biosci. Biotechnol. Biochem. 2009;73:140-146). In this article, we describe the identification and expression of the tomato acidic α-mannosidase gene using the yeast-expression system. The α-mannosidase-gene located at chomosome 6 is a 10 kb spanned containing 30 exons. The gene-encoded-protein is single polypeptide chain of 1,028 amino acids containing glycosyl hydrolase domain-38 with predicted molecular mass of 116 kDa. The recombinant enzyme showed maximum activity at pH 5.5, and was almost completely inhibited by both of 1-deoxymannojirimycin and swainsonine. The recombinant α-mannosidase, like the native enzyme, could cleave α1-2, 1-3 and 1-6 mannosidic linkage from both high-mannose and truncated complex-type N-glycans. A molecular 3D modelling shows that catalytically important residues of animal lysosomal α-mannosidase could be superimposed on those of tomato α-mannosidase, suggesting that active site conformation is highly conserved between plant acidic α-mannosidase and animal lysosomal α-mannosidase.

Original languageEnglish
Pages (from-to)603-616
Number of pages14
JournalJournal of Biochemistry
Volume148
Issue number5
DOIs
Publication statusPublished - Nov 2010

Fingerprint

Mannosidases
Lycopersicon esculentum
Fruits
Polysaccharides
Fruit
Genes
Animals
1-Deoxynojirimycin
Swainsonine
Molecular mass
Hydrolases
Enzymes
Mannose
Yeast
Conformations
Exons
Catalytic Domain
Glycoproteins
Yeasts

Keywords

  • acidic α-mannosidase
  • glycogene
  • Lycopersicon esculentum
  • plant N-glycan

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

Molecular characterization of plant acidic α-mannosidase, a member of glycosylhydrolase family 38, involved in the turnover of N-glycans during tomato fruit ripening. / Hossain, Md Anowar; Nakano, Ryohei; Nakamura, Kosuke; Hossain, Md Tofazzal; Kimura, Yoshinobu.

In: Journal of Biochemistry, Vol. 148, No. 5, 11.2010, p. 603-616.

Research output: Contribution to journalArticle

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