Molecular characterization of intact, but cryptic, flagellin genes in the genus Shigella

Akira Tominaga, Mohamed Abdel Hakim Mahmoud, Takafumi Mukaihara, Masatoshi Enomoto

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

Flagellin genes (fliC) were detected in two species of the genus Shigella. The fliC(SF) gene cloned from Shigella flexneri produced normal-type flagella in an Escherichia coli ΔfliC strain while the fliC(SS) genes from two Shigella sonnel strains produced curly-type flagella and their expression is repressible by Salmonella FIjA repressor. The fliC(SF) gene (1650 bp) shared high similarity with the E. coli fliC(E) gene not only in the 5' and 3' constant sequences but also in the upstream and downstream sequences. The fliC(SS) genes, (1572 bp) shared high similarity with the Salmonella typhimurium fliC(S) gene in the operator and 3' constant sequences and also shared high similarity with the fliC(E) gene in the downstream sequence, suggesting that the fliC(SS) gene has undergone horizontal transfer and recombination. Differences in nucleotide sequences of the central variable regions among the four fliC genes, including fliC(E) and fliC(S), suggest that they started differentiation in each lineage approximately 80 million years ago. Loss of motility in Shigella seems to be evolutionarily a recent event.

Original languageEnglish
Pages (from-to)277-285
Number of pages9
JournalMolecular Microbiology
Volume12
Issue number2
DOIs
Publication statusPublished - Apr 1994

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Flagellin
Shigella
Genes
Flagella
Escherichia coli
Shigella flexneri
Salmonella typhimurium
Salmonella
Genetic Recombination

ASJC Scopus subject areas

  • Molecular Biology
  • Microbiology

Cite this

Molecular characterization of intact, but cryptic, flagellin genes in the genus Shigella. / Tominaga, Akira; Mahmoud, Mohamed Abdel Hakim; Mukaihara, Takafumi; Enomoto, Masatoshi.

In: Molecular Microbiology, Vol. 12, No. 2, 04.1994, p. 277-285.

Research output: Contribution to journalArticle

Tominaga, Akira ; Mahmoud, Mohamed Abdel Hakim ; Mukaihara, Takafumi ; Enomoto, Masatoshi. / Molecular characterization of intact, but cryptic, flagellin genes in the genus Shigella. In: Molecular Microbiology. 1994 ; Vol. 12, No. 2. pp. 277-285.
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