Abstract
Flagellin genes (fliC) were detected in two species of the genus Shigella. The fliC(SF) gene cloned from Shigella flexneri produced normal-type flagella in an Escherichia coli ΔfliC strain while the fliC(SS) genes from two Shigella sonnel strains produced curly-type flagella and their expression is repressible by Salmonella FIjA repressor. The fliC(SF) gene (1650 bp) shared high similarity with the E. coli fliC(E) gene not only in the 5' and 3' constant sequences but also in the upstream and downstream sequences. The fliC(SS) genes, (1572 bp) shared high similarity with the Salmonella typhimurium fliC(S) gene in the operator and 3' constant sequences and also shared high similarity with the fliC(E) gene in the downstream sequence, suggesting that the fliC(SS) gene has undergone horizontal transfer and recombination. Differences in nucleotide sequences of the central variable regions among the four fliC genes, including fliC(E) and fliC(S), suggest that they started differentiation in each lineage approximately 80 million years ago. Loss of motility in Shigella seems to be evolutionarily a recent event.
| Original language | English |
|---|---|
| Pages (from-to) | 277-285 |
| Number of pages | 9 |
| Journal | Molecular Microbiology |
| Volume | 12 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Apr 1994 |
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ASJC Scopus subject areas
- Molecular Biology
- Microbiology
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Molecular characterization of intact, but cryptic, flagellin genes in the genus Shigella. / Tominaga, Akira; Mahmoud, Mohamed Abdel Hakim; Mukaihara, Takafumi; Enomoto, Masatoshi.
In: Molecular Microbiology, Vol. 12, No. 2, 04.1994, p. 277-285.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Molecular characterization of intact, but cryptic, flagellin genes in the genus Shigella
AU - Tominaga, Akira
AU - Mahmoud, Mohamed Abdel Hakim
AU - Mukaihara, Takafumi
AU - Enomoto, Masatoshi
PY - 1994/4
Y1 - 1994/4
N2 - Flagellin genes (fliC) were detected in two species of the genus Shigella. The fliC(SF) gene cloned from Shigella flexneri produced normal-type flagella in an Escherichia coli ΔfliC strain while the fliC(SS) genes from two Shigella sonnel strains produced curly-type flagella and their expression is repressible by Salmonella FIjA repressor. The fliC(SF) gene (1650 bp) shared high similarity with the E. coli fliC(E) gene not only in the 5' and 3' constant sequences but also in the upstream and downstream sequences. The fliC(SS) genes, (1572 bp) shared high similarity with the Salmonella typhimurium fliC(S) gene in the operator and 3' constant sequences and also shared high similarity with the fliC(E) gene in the downstream sequence, suggesting that the fliC(SS) gene has undergone horizontal transfer and recombination. Differences in nucleotide sequences of the central variable regions among the four fliC genes, including fliC(E) and fliC(S), suggest that they started differentiation in each lineage approximately 80 million years ago. Loss of motility in Shigella seems to be evolutionarily a recent event.
AB - Flagellin genes (fliC) were detected in two species of the genus Shigella. The fliC(SF) gene cloned from Shigella flexneri produced normal-type flagella in an Escherichia coli ΔfliC strain while the fliC(SS) genes from two Shigella sonnel strains produced curly-type flagella and their expression is repressible by Salmonella FIjA repressor. The fliC(SF) gene (1650 bp) shared high similarity with the E. coli fliC(E) gene not only in the 5' and 3' constant sequences but also in the upstream and downstream sequences. The fliC(SS) genes, (1572 bp) shared high similarity with the Salmonella typhimurium fliC(S) gene in the operator and 3' constant sequences and also shared high similarity with the fliC(E) gene in the downstream sequence, suggesting that the fliC(SS) gene has undergone horizontal transfer and recombination. Differences in nucleotide sequences of the central variable regions among the four fliC genes, including fliC(E) and fliC(S), suggest that they started differentiation in each lineage approximately 80 million years ago. Loss of motility in Shigella seems to be evolutionarily a recent event.
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U2 - 10.1111/j.1365-2958.1994.tb01016.x
DO - 10.1111/j.1365-2958.1994.tb01016.x
M3 - Article
VL - 12
SP - 277
EP - 285
JO - Molecular Microbiology
JF - Molecular Microbiology
SN - 0950-382X
IS - 2
ER -