Molecular characterization of infectious bursal disease viruses detected in vaccinated commercial broiler flocks in Lusaka, Zambia

Kunda Ndashe, Edgar Simulundu, Bernard M. Hang’ombe, Ladslav Moonga, Hirohito Ogawa, Ayato Takada, Aaron S. Mweene

Research output: Contribution to journalArticle

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Abstract

Infectious bursal disease (IBD) is an acute, highly contagious, and immunosuppressive viral disease of young chickens and remains one of the economically most important diseases threatening the poultry industry worldwide. In this study, 16 and 11 nucleotide sequences of the VP2 hypervariable region (VP2-HVR) and part of VP1, respectively, of IBD virus (IBDV) detected in vaccinated broiler chickens in Lusaka in 2012 were determined. Phylogenetic analysis revealed that these Zambian IBDVs separated into three genotypes of very virulent (VV) IBDVs. Although the majority of these viruses belonged to the African VV type (VV1), which consisted of viruses from West Africa, South Africa and Zambia, one virus belonged to the East African VV type (VV2). Interestingly, a Zambian IBDV belonging to the VV3 genotype (composed of viruses from several continents) clustered with attenuated vaccine strains. Although sequence analysis of VP2-HVR showed that all detected Zambian IBDVs had conserved putative virulence marker amino acids (i.e., 222A, 242I, 256I, 294I and 299S), one virus had two unique amino acid substitutions, N280S and E300A. This study demonstrates the diversity of Zambian IBDVs and documents for the first time the possible involvement of attenuated vaccine strains in the epidemiology of IBD in Zambia. Strict biosecurity of poultry farms, monitoring of live vaccine use in the field, surveillance and characterization of IBDV in poultry and development of a vaccine from local or regional IBDV field strains are recommended for improved IBD control in Zambia.

Original languageEnglish
Pages (from-to)513-519
Number of pages7
JournalArchives of Virology
Volume161
Issue number3
DOIs
Publication statusPublished - Mar 1 2016

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Infectious bursal disease virus
Zambia
Viruses
Communicable Diseases
Attenuated Vaccines
Poultry
Chickens
Vaccines
Poultry Diseases
Genotype
Western Africa
Virus Diseases
Amino Acid Substitution
Immunosuppressive Agents
South Africa
Sequence Analysis
Virulence
Industry
Epidemiology
Amino Acids

ASJC Scopus subject areas

  • Virology

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Molecular characterization of infectious bursal disease viruses detected in vaccinated commercial broiler flocks in Lusaka, Zambia. / Ndashe, Kunda; Simulundu, Edgar; Hang’ombe, Bernard M.; Moonga, Ladslav; Ogawa, Hirohito; Takada, Ayato; Mweene, Aaron S.

In: Archives of Virology, Vol. 161, No. 3, 01.03.2016, p. 513-519.

Research output: Contribution to journalArticle

Ndashe, Kunda ; Simulundu, Edgar ; Hang’ombe, Bernard M. ; Moonga, Ladslav ; Ogawa, Hirohito ; Takada, Ayato ; Mweene, Aaron S. / Molecular characterization of infectious bursal disease viruses detected in vaccinated commercial broiler flocks in Lusaka, Zambia. In: Archives of Virology. 2016 ; Vol. 161, No. 3. pp. 513-519.
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