Molecular characterization of Chlorella chromosomes: screening of bent DNAs.

Akiba, Y. Noutoshi, S. Maki, T. Higashiyama, T. Yamada

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

As a model for plant-type chromosomes, we have been characterizing molecular organization of Chlorella vulgaris (C-169) chromosome I. To locate structurally important chromosome regions, bent DNA fragments of this chromosome were systematically obtained and analyzed. By two-dimensional polyacrylamide gel electrophoresis, 150 clones containing bent DNA fragments were screened from C. vulgaris genomic libraries. These clones were classified into 3 groups by Southern hybridization. A group that hybridized with all Chlorella chromosomes contained fragments carrying a retroposon-like element. A clone (p2DS-66A) derived from chromosome VI strongly hybridized with chromosome I as well as the others. The corresponding chromosome I sequences (pI-21 and pI-31) were analyzed in detail.

Original languageEnglish
Pages (from-to)73-74
Number of pages2
JournalNucleic acids symposium series
Issue number34
Publication statusPublished - 1995
Externally publishedYes

ASJC Scopus subject areas

  • Medicine(all)

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