Molecular characterization of Chlorella chromosomes

screening of bent DNAs.

Akiba, Yoshiteru Noutoshi, S. Maki, T. Higashiyama, T. Yamada

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

As a model for plant-type chromosomes, we have been characterizing molecular organization of Chlorella vulgaris (C-169) chromosome I. To locate structurally important chromosome regions, bent DNA fragments of this chromosome were systematically obtained and analyzed. By two-dimensional polyacrylamide gel electrophoresis, 150 clones containing bent DNA fragments were screened from C. vulgaris genomic libraries. These clones were classified into 3 groups by Southern hybridization. A group that hybridized with all Chlorella chromosomes contained fragments carrying a retroposon-like element. A clone (p2DS-66A) derived from chromosome VI strongly hybridized with chromosome I as well as the others. The corresponding chromosome I sequences (pI-21 and pI-31) were analyzed in detail.

Original languageEnglish
Pages (from-to)73-74
Number of pages2
JournalNucleic acids symposium series
Issue number34
Publication statusPublished - 1995
Externally publishedYes

Fingerprint

Chlorella
Chromosomes
DNA
Chlorella vulgaris
Clone Cells
Plant Chromosomes
Chromosomes, Human, 6-12 and X
Retroelements
Genomic Library
Electrophoresis, Gel, Two-Dimensional

Cite this

Molecular characterization of Chlorella chromosomes : screening of bent DNAs. / Akiba, ; Noutoshi, Yoshiteru; Maki, S.; Higashiyama, T.; Yamada, T.

In: Nucleic acids symposium series, No. 34, 1995, p. 73-74.

Research output: Contribution to journalArticle

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