Molecular basis of late-flowering phenotype caused by dominant epi-alleles of the FWA locus in Arabidopsis

Yoko Ikeda, Yasushi Kobayashi, Ayako Yamaguchi, Mitsutomo Abe, Takashi Araki

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

The late-flowering phenotype of dominant fwa mutants is caused by hypomethylation in the FWA locus leading to ectopic expression of a homeodomain leucine zipper (HD-ZIP) protein. However, little is known about whether FWA has any role in regulation of flowering and how ectopically expressed FWA delays flowering. Through analysis of FWA expression in wild-type seedlings, it was shown that FWA is not expressed during the vegetative phase. This suggests that FWA has no role in flowering. The previous reports that fwa suppressed the precocious-flowering phenotype of plants overexpressing FLOWERING LOCUS T (FT) suggest that the flowering pathway(s) either at and/or downstream of FT is blocked by FWA. Comparison of gene expression profiles in three genetic backgrounds ectopically expressing FWA and their respective wild types failed to detect common changes, ruling out the possibility that FWA acts through transcriptional misregulation. Yeast two-hybrid analysis and in vitro pull-down assay showed that FWA protein can specifically interact with FT protein. The importance of protein interaction with FT in delaying flowering was supported by studies involving N-terminal and C-terminal truncations of FWA. The C-terminal truncation with abolished interaction did not delay flowering when overexpressed, while the N-terminal truncation, which retains interaction, did. Specific interaction of FWA with FT enabled us to use FWA protein as a specific inhibitor of FT protein function. Through tissue-specific ectopic expression of FWA, further support for the shoot apex being the site of action of FT protein was provided.

Original languageEnglish
Pages (from-to)205-220
Number of pages16
JournalPlant and Cell Physiology
Volume48
Issue number2
DOIs
Publication statusPublished - Feb 2007
Externally publishedYes

Fingerprint

Arabidopsis
Alleles
flowering
alleles
Phenotype
phenotype
loci
Proteins
proteins
Leucine Zippers
leucine zipper
two hybrid system techniques
Seedlings
Transcriptome
genetic background
Yeasts
yeasts
gene expression
mutants
shoots

Keywords

  • Arabidopsis
  • Epi-mutant
  • Flowering
  • FT
  • FWA
  • Protein interaction

ASJC Scopus subject areas

  • Plant Science
  • Physiology
  • Cell Biology

Cite this

Molecular basis of late-flowering phenotype caused by dominant epi-alleles of the FWA locus in Arabidopsis. / Ikeda, Yoko; Kobayashi, Yasushi; Yamaguchi, Ayako; Abe, Mitsutomo; Araki, Takashi.

In: Plant and Cell Physiology, Vol. 48, No. 2, 02.2007, p. 205-220.

Research output: Contribution to journalArticle

Ikeda, Yoko ; Kobayashi, Yasushi ; Yamaguchi, Ayako ; Abe, Mitsutomo ; Araki, Takashi. / Molecular basis of late-flowering phenotype caused by dominant epi-alleles of the FWA locus in Arabidopsis. In: Plant and Cell Physiology. 2007 ; Vol. 48, No. 2. pp. 205-220.
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