Molecular basis for premature senescence induced by surfactants in normal human cells

Yoshimi Yamakami, Kensuke Miki, Ryuzo Yonekura, Ikuru Kudo, Michihiko Fujii, Dai Ayusawa

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Sublethal doses of surfactants as exemplified by NP-40 clearly induce premature senescence in normal human cells. To understand molecular basis for this phenomenon, we tried to suppress it with use of various inhibitors. An inhibitor of p38 of the MAPK family almost completely suppressed growth arrest and morphological changes induced by surfactants; however, other inhibitors tested had no effect. Oleic acid, a weak inducer of premature senescence, was found to suppress the effect of NP-40. Fluoresceinlabeled oleic acid rapidly bound to the cell surface, and this binding was clearly blocked by pre-treatment with surfactants, suggesting that surfactants and oleic acid compete for binding to the cell surface. Moderate concentrations of cycloheximide, an inhibitor of protein synthesis, also suppressed the senescent features induced by NP-40. These results suggest that surfactants activate p38 signaling pathway by binding to the cell surface, and induce cellular senescence.

Original languageEnglish
Pages (from-to)2022-2029
Number of pages8
JournalBioscience, Biotechnology and Biochemistry
Volume78
Issue number12
DOIs
Publication statusPublished - Jan 1 2014
Externally publishedYes

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Keywords

  • Cellular senescence
  • Oleic acid
  • P38
  • Surfactant
  • Unbalanced growth

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biotechnology
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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