Molecular and structural patterns of bone regeneration in surgically created defects containing bone substitutes

Ibrahim Elgali, Kazuyo Igawa, Anders Palmquist, Maria Lennerås, Wei Xia, Sungjin Choi, Ung Il Chung, Omar Omar, Peter Thomsen

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Several biomaterials have been introduced for bone augmentation. However, information is lacking about the mechanisms of bone regeneration and/or integration of these materials in the recipient bone. This study aimed to determine the molecular and structural events in bone defects after augmentation with synthetic tetrapod-shaped calcium phosphate (Tetrabone; TetraB) compared with natural deproteinized bovine bone (DBB). Defects were created in the epiphyses of rat femurs and filled with TetraB or DBB or left empty (Sham). After 3, 6, 14 and 28 d, samples were harvested for histology, histomorphometry, ultrastructure and gene expression analyses. At 3 d, higher expressions of bone formation (ALP and OC) and remodeling (CatK) genes were detected in TetraB compared with DBB and Sham. Downregulation of bone remodeling genes (TRAP and CatK) was detected in DBB as compared to Sham after 14 d. Histomorphometry at 6 and 14 d demonstrated greater bone contact with the granules in TetraB. At 28 d, a larger bone area per defect was found in TetraB. The present experiments show that a synthetic substitute, consisting of α-tricalcium and octacalcium phosphates, induces early osteogenic and osteoclastic activities and promotes bone formation in trabecular bone defects.

Original languageEnglish
Pages (from-to)3229-3242
Number of pages14
JournalBiomaterials
Volume35
Issue number10
DOIs
Publication statusPublished - Mar 2014
Externally publishedYes

Fingerprint

Bone Substitutes
Bone Regeneration
Bone
Bone and Bones
Defects
Osteogenesis
Epiphyses
Bone Remodeling
Biocompatible Materials
Genes
Femur
Histology
Down-Regulation
Calcium phosphate
Gene Expression
Gene expression
Biomaterials

Keywords

  • α-Tricalcium phosphate
  • Deproteinized bovine bone
  • Gene expression
  • In vivo
  • Octacalcium phosphate
  • Ultrastructure

ASJC Scopus subject areas

  • Bioengineering
  • Ceramics and Composites
  • Biophysics
  • Biomaterials
  • Mechanics of Materials

Cite this

Molecular and structural patterns of bone regeneration in surgically created defects containing bone substitutes. / Elgali, Ibrahim; Igawa, Kazuyo; Palmquist, Anders; Lennerås, Maria; Xia, Wei; Choi, Sungjin; Chung, Ung Il; Omar, Omar; Thomsen, Peter.

In: Biomaterials, Vol. 35, No. 10, 03.2014, p. 3229-3242.

Research output: Contribution to journalArticle

Elgali, I, Igawa, K, Palmquist, A, Lennerås, M, Xia, W, Choi, S, Chung, UI, Omar, O & Thomsen, P 2014, 'Molecular and structural patterns of bone regeneration in surgically created defects containing bone substitutes', Biomaterials, vol. 35, no. 10, pp. 3229-3242. https://doi.org/10.1016/j.biomaterials.2013.12.084
Elgali, Ibrahim ; Igawa, Kazuyo ; Palmquist, Anders ; Lennerås, Maria ; Xia, Wei ; Choi, Sungjin ; Chung, Ung Il ; Omar, Omar ; Thomsen, Peter. / Molecular and structural patterns of bone regeneration in surgically created defects containing bone substitutes. In: Biomaterials. 2014 ; Vol. 35, No. 10. pp. 3229-3242.
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