Molecular and physical mapping of a barley gene on chromosome arm 1HL that causes sterility in hybrids with wheat

Shin Taketa, Masayuki Choda, Ryoko Ohashi, Masahiko Ichii, Kazuyoshi Takeda

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Addition of the long arm of barley chromosome 1H (1HL) to wheat causes severe meiotic abnormalities and complete sterility of the plants. To map the barley gene responsible for the 1H-induced sterility of wheat, a series of addition lines of translocated 1H chromosomes were developed from the crosses between the wheat 'Shinchunaga' and five reciprocal translocation lines derived from the barley line St.13559. Examination of the seed fertility of the addition lines revealed that the sterility gene is located in the interstitial 25% region of the 1HL arm. The genetic location of the sterility gene was also estimated by physically mapping sequence-tagged site (STS) markers and simple-sequence repeat (SSR) markers with known map locations. The sterility gene is designated Shw (sterility in hybrids with wheat). Comparison of the present physical map of 1HL with two previously published genetic maps revealed a paucity of markers in the proximal 30% region and non-random distribution of SSR markers. Two inconsistencies in marker order were found between the present physical map and the consensus genetic map of group 1 chromosomes of Triticeae. On the basis of the effects on meiosis and chromosomal location, the relationship of the present sterility gene with other fertility-related genes of Triticeae is discussed.

Original languageEnglish
Pages (from-to)617-625
Number of pages9
JournalGenome
Volume45
Issue number4
DOIs
Publication statusPublished - 2002
Externally publishedYes

Fingerprint

physical chromosome mapping
Hordeum
Infertility
Triticum
chromosome mapping
Chromosomes
barley
chromosomes
wheat
Genes
genes
Microsatellite Repeats
Fertility
microsatellite repeats
translocation lines
plant fertility
reciprocal translocation
Sequence Tagged Sites
Chromosomes, Human, Pair 1
meiosis

Keywords

  • Hordeum vulgare
  • Molecular markers
  • Sterility
  • Translocation
  • Wheat-barley chromosome addition line

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biotechnology
  • Genetics
  • Genetics(clinical)

Cite this

Molecular and physical mapping of a barley gene on chromosome arm 1HL that causes sterility in hybrids with wheat. / Taketa, Shin; Choda, Masayuki; Ohashi, Ryoko; Ichii, Masahiko; Takeda, Kazuyoshi.

In: Genome, Vol. 45, No. 4, 2002, p. 617-625.

Research output: Contribution to journalArticle

Taketa, Shin ; Choda, Masayuki ; Ohashi, Ryoko ; Ichii, Masahiko ; Takeda, Kazuyoshi. / Molecular and physical mapping of a barley gene on chromosome arm 1HL that causes sterility in hybrids with wheat. In: Genome. 2002 ; Vol. 45, No. 4. pp. 617-625.
@article{9adb741b40984d949ccc7375c14c5a69,
title = "Molecular and physical mapping of a barley gene on chromosome arm 1HL that causes sterility in hybrids with wheat",
abstract = "Addition of the long arm of barley chromosome 1H (1HL) to wheat causes severe meiotic abnormalities and complete sterility of the plants. To map the barley gene responsible for the 1H-induced sterility of wheat, a series of addition lines of translocated 1H chromosomes were developed from the crosses between the wheat 'Shinchunaga' and five reciprocal translocation lines derived from the barley line St.13559. Examination of the seed fertility of the addition lines revealed that the sterility gene is located in the interstitial 25{\%} region of the 1HL arm. The genetic location of the sterility gene was also estimated by physically mapping sequence-tagged site (STS) markers and simple-sequence repeat (SSR) markers with known map locations. The sterility gene is designated Shw (sterility in hybrids with wheat). Comparison of the present physical map of 1HL with two previously published genetic maps revealed a paucity of markers in the proximal 30{\%} region and non-random distribution of SSR markers. Two inconsistencies in marker order were found between the present physical map and the consensus genetic map of group 1 chromosomes of Triticeae. On the basis of the effects on meiosis and chromosomal location, the relationship of the present sterility gene with other fertility-related genes of Triticeae is discussed.",
keywords = "Hordeum vulgare, Molecular markers, Sterility, Translocation, Wheat-barley chromosome addition line",
author = "Shin Taketa and Masayuki Choda and Ryoko Ohashi and Masahiko Ichii and Kazuyoshi Takeda",
year = "2002",
doi = "10.1139/g02-024",
language = "English",
volume = "45",
pages = "617--625",
journal = "Genome / National Research Council Canada = Genome / Conseil national de recherches Canada",
issn = "0831-2796",
publisher = "National Research Council of Canada",
number = "4",

}

TY - JOUR

T1 - Molecular and physical mapping of a barley gene on chromosome arm 1HL that causes sterility in hybrids with wheat

AU - Taketa, Shin

AU - Choda, Masayuki

AU - Ohashi, Ryoko

AU - Ichii, Masahiko

AU - Takeda, Kazuyoshi

PY - 2002

Y1 - 2002

N2 - Addition of the long arm of barley chromosome 1H (1HL) to wheat causes severe meiotic abnormalities and complete sterility of the plants. To map the barley gene responsible for the 1H-induced sterility of wheat, a series of addition lines of translocated 1H chromosomes were developed from the crosses between the wheat 'Shinchunaga' and five reciprocal translocation lines derived from the barley line St.13559. Examination of the seed fertility of the addition lines revealed that the sterility gene is located in the interstitial 25% region of the 1HL arm. The genetic location of the sterility gene was also estimated by physically mapping sequence-tagged site (STS) markers and simple-sequence repeat (SSR) markers with known map locations. The sterility gene is designated Shw (sterility in hybrids with wheat). Comparison of the present physical map of 1HL with two previously published genetic maps revealed a paucity of markers in the proximal 30% region and non-random distribution of SSR markers. Two inconsistencies in marker order were found between the present physical map and the consensus genetic map of group 1 chromosomes of Triticeae. On the basis of the effects on meiosis and chromosomal location, the relationship of the present sterility gene with other fertility-related genes of Triticeae is discussed.

AB - Addition of the long arm of barley chromosome 1H (1HL) to wheat causes severe meiotic abnormalities and complete sterility of the plants. To map the barley gene responsible for the 1H-induced sterility of wheat, a series of addition lines of translocated 1H chromosomes were developed from the crosses between the wheat 'Shinchunaga' and five reciprocal translocation lines derived from the barley line St.13559. Examination of the seed fertility of the addition lines revealed that the sterility gene is located in the interstitial 25% region of the 1HL arm. The genetic location of the sterility gene was also estimated by physically mapping sequence-tagged site (STS) markers and simple-sequence repeat (SSR) markers with known map locations. The sterility gene is designated Shw (sterility in hybrids with wheat). Comparison of the present physical map of 1HL with two previously published genetic maps revealed a paucity of markers in the proximal 30% region and non-random distribution of SSR markers. Two inconsistencies in marker order were found between the present physical map and the consensus genetic map of group 1 chromosomes of Triticeae. On the basis of the effects on meiosis and chromosomal location, the relationship of the present sterility gene with other fertility-related genes of Triticeae is discussed.

KW - Hordeum vulgare

KW - Molecular markers

KW - Sterility

KW - Translocation

KW - Wheat-barley chromosome addition line

UR - http://www.scopus.com/inward/record.url?scp=0036433414&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036433414&partnerID=8YFLogxK

U2 - 10.1139/g02-024

DO - 10.1139/g02-024

M3 - Article

C2 - 12175064

AN - SCOPUS:0036433414

VL - 45

SP - 617

EP - 625

JO - Genome / National Research Council Canada = Genome / Conseil national de recherches Canada

JF - Genome / National Research Council Canada = Genome / Conseil national de recherches Canada

SN - 0831-2796

IS - 4

ER -