Molecular and cellular basis of regeneration and tissue repair: Dissecting insect leg regeneration through RNA interference

T. Nakamura, T. Mito, T. Bando, H. Ohuchi, S. Noji

Research output: Contribution to journalReview article

51 Citations (Scopus)


Nymphs of hemimetabolous insects such as cockroaches and crickets exhibit a remarkable capacity for regenerating complex structures from damaged legs. Until recent years, however, approaches to elucidate the molecular mechanisms underlying the leg regeneration process have been lacking. Taking the cricket Gryllus bimaculatus as a model, we found that a phenotype related to regeneration frequently appears during leg regeneration, even though no phenotype is induced by RNA interference (RNAi) in the cricket nymph, designated as regeneration-dependent RNAi. Since then, we have investigated the functions of various genes encoding signaling factors and cellular adhesion proteins like Fat and Dachsous during leg regeneration. In this review, we summarize the classical knowledge about insect leg regeneration and introduce recent advances concerning the signaling cascades required for regenerating a leg. Our results provide clues to the mechanisms of regeneration which are relevant to vertebrate systems.

Original languageEnglish
Pages (from-to)64-72
Number of pages9
JournalCellular and Molecular Life Sciences
Issue number1
Publication statusPublished - Jan 1 2008
Externally publishedYes



  • Fat signaling
  • Gryllus bimaculatus
  • Legs
  • Positional information
  • Regeneration
  • Wnt signaling

ASJC Scopus subject areas

  • Molecular Medicine
  • Molecular Biology
  • Pharmacology
  • Cellular and Molecular Neuroscience
  • Cell Biology

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