TY - JOUR
T1 - Molecular and biochemical differences in leaf explants and the implication for regeneration ability in rorippa aquatica (Brassicaceae)
AU - Amano, Rumi
AU - Momoi, Risa
AU - Omata, Emi
AU - Nakahara, Taiga
AU - Kaminoyama, Kaori
AU - Kojima, Mikiko
AU - Takebayashi, Yumiko
AU - Ikematsu, Shuka
AU - Okegawa, Yuki
AU - Sakamoto, Tomoaki
AU - Kasahara, Hiroyuki
AU - Sakakibara, Hitoshi
AU - Motohashi, Ken
AU - Kimura, Seisuke
N1 - Funding Information:
Funding: This research was funded by the Japan Society for the Promotion of Science (grant numbers 18H04787 and 18H04844) to S.K., Japan Society for the Promotion of Science (grant number 17J03754) to R.A., and Ministry of Education, Culture, Sports, Science and Technology (grant number S1511023) to S.K.
Publisher Copyright:
© 2020 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2020/10
Y1 - 2020/10
N2 - Plants have a high regeneration capacity and some plant species can regenerate clone plants, called plantlets, from detached vegetative organs. We previously outlined the molecular mechanisms underlying plantlet regeneration from Rorippa aquatica (Brassicaceae) leaf explants. However, the fundamental difference between the plant species that can and cannot regenerate plantlets from vegetative organs remains unclear. Here, we hypothesized that the viability of leaf explants is a key factor affecting the regeneration capacity of R. aquatica. To test this hypothesis, the viability of R. aquatica and Arabidopsis thaliana leaf explants were compared, with respect to the maintenance of photosynthetic activity, senescence, and immune response. Time-course analyses of photosynthetic activity revealed that R. aquatica leaf explants can survive longer than those of A. thaliana. Endogenous abscisic acid (ABA) and jasmonic acid (JA) were found at low levels in leaf explant of R. aquatica. Time-course transcriptome analysis of R. aquatica and A. thaliana leaf explants suggested that senescence was suppressed at the transcriptional level in R. aquatica. Application of exogenous ABA reduced the efficiency of plantlet regeneration. Overall, our results propose that in nature, plant species that can regenerate in nature can survive for a long time.
AB - Plants have a high regeneration capacity and some plant species can regenerate clone plants, called plantlets, from detached vegetative organs. We previously outlined the molecular mechanisms underlying plantlet regeneration from Rorippa aquatica (Brassicaceae) leaf explants. However, the fundamental difference between the plant species that can and cannot regenerate plantlets from vegetative organs remains unclear. Here, we hypothesized that the viability of leaf explants is a key factor affecting the regeneration capacity of R. aquatica. To test this hypothesis, the viability of R. aquatica and Arabidopsis thaliana leaf explants were compared, with respect to the maintenance of photosynthetic activity, senescence, and immune response. Time-course analyses of photosynthetic activity revealed that R. aquatica leaf explants can survive longer than those of A. thaliana. Endogenous abscisic acid (ABA) and jasmonic acid (JA) were found at low levels in leaf explant of R. aquatica. Time-course transcriptome analysis of R. aquatica and A. thaliana leaf explants suggested that senescence was suppressed at the transcriptional level in R. aquatica. Application of exogenous ABA reduced the efficiency of plantlet regeneration. Overall, our results propose that in nature, plant species that can regenerate in nature can survive for a long time.
KW - North American Lake Cress
KW - Phytohormones
KW - Plantlet
KW - Propagation leaf explant
KW - Vegetative
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U2 - 10.3390/plants9101372
DO - 10.3390/plants9101372
M3 - Article
AN - SCOPUS:85092705688
VL - 9
SP - 1
EP - 15
JO - Plants
JF - Plants
SN - 2223-7747
IS - 10
M1 - 1372
ER -