Molecular Anatomy of a Trafficking Organelle

Shigeo Takamori, Matthew Holt, Katinka Stenius, Edward A. Lemke, Mads Grønborg, Dietmar Riedel, Henning Urlaub, Stephan Schenck, Britta Brügger, Philippe Ringler, Shirley A. Müller, Burkhard Rammner, Frauke Gräter, Jochen S. Hub, Bert L. De Groot, Gottfried Mieskes, Yoshinori Moriyama, Jürgen Klingauf, Helmut Grubmüller, John HeuserFelix Wieland, Reinhard Jahn

Research output: Contribution to journalArticle

1343 Citations (Scopus)

Abstract

Membrane traffic in eukaryotic cells involves transport of vesicles that bud from a donor compartment and fuse with an acceptor compartment. Common principles of budding and fusion have emerged, and many of the proteins involved in these events are now known. However, a detailed picture of an entire trafficking organelle is not yet available. Using synaptic vesicles as a model, we have now determined the protein and lipid composition; measured vesicle size, density, and mass; calculated the average protein and lipid mass per vesicle; and determined the copy number of more than a dozen major constituents. A model has been constructed that integrates all quantitative data and includes structural models of abundant proteins. Synaptic vesicles are dominated by proteins, possess a surprising diversity of trafficking proteins, and, with the exception of the V-ATPase that is present in only one to two copies, contain numerous copies of proteins essential for membrane traffic and neurotransmitter uptake.

Original languageEnglish
Pages (from-to)831-846
Number of pages16
JournalCell
Volume127
Issue number4
DOIs
Publication statusPublished - Nov 17 2006

Fingerprint

Organelles
Anatomy
Synaptic Vesicles
Proteins
Lipids
Transport Vesicles
Structural Models
Eukaryotic Cells
Protein Transport
Membranes
Neurotransmitter Agents
Adenosine Triphosphatases
Membrane Proteins
Electric fuses
Fusion reactions
Chemical analysis

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

Takamori, S., Holt, M., Stenius, K., Lemke, E. A., Grønborg, M., Riedel, D., ... Jahn, R. (2006). Molecular Anatomy of a Trafficking Organelle. Cell, 127(4), 831-846. https://doi.org/10.1016/j.cell.2006.10.030

Molecular Anatomy of a Trafficking Organelle. / Takamori, Shigeo; Holt, Matthew; Stenius, Katinka; Lemke, Edward A.; Grønborg, Mads; Riedel, Dietmar; Urlaub, Henning; Schenck, Stephan; Brügger, Britta; Ringler, Philippe; Müller, Shirley A.; Rammner, Burkhard; Gräter, Frauke; Hub, Jochen S.; De Groot, Bert L.; Mieskes, Gottfried; Moriyama, Yoshinori; Klingauf, Jürgen; Grubmüller, Helmut; Heuser, John; Wieland, Felix; Jahn, Reinhard.

In: Cell, Vol. 127, No. 4, 17.11.2006, p. 831-846.

Research output: Contribution to journalArticle

Takamori, S, Holt, M, Stenius, K, Lemke, EA, Grønborg, M, Riedel, D, Urlaub, H, Schenck, S, Brügger, B, Ringler, P, Müller, SA, Rammner, B, Gräter, F, Hub, JS, De Groot, BL, Mieskes, G, Moriyama, Y, Klingauf, J, Grubmüller, H, Heuser, J, Wieland, F & Jahn, R 2006, 'Molecular Anatomy of a Trafficking Organelle', Cell, vol. 127, no. 4, pp. 831-846. https://doi.org/10.1016/j.cell.2006.10.030
Takamori S, Holt M, Stenius K, Lemke EA, Grønborg M, Riedel D et al. Molecular Anatomy of a Trafficking Organelle. Cell. 2006 Nov 17;127(4):831-846. https://doi.org/10.1016/j.cell.2006.10.030
Takamori, Shigeo ; Holt, Matthew ; Stenius, Katinka ; Lemke, Edward A. ; Grønborg, Mads ; Riedel, Dietmar ; Urlaub, Henning ; Schenck, Stephan ; Brügger, Britta ; Ringler, Philippe ; Müller, Shirley A. ; Rammner, Burkhard ; Gräter, Frauke ; Hub, Jochen S. ; De Groot, Bert L. ; Mieskes, Gottfried ; Moriyama, Yoshinori ; Klingauf, Jürgen ; Grubmüller, Helmut ; Heuser, John ; Wieland, Felix ; Jahn, Reinhard. / Molecular Anatomy of a Trafficking Organelle. In: Cell. 2006 ; Vol. 127, No. 4. pp. 831-846.
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