Molecular anatomy of a small chromosome in the green alga Chlorella vulgaris

Yoshiteru Noutoshi, Yukari Ito, Shinsaku Kanetani, Makoto Fujie, Shouji Usami, Takashi Yamada

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

A contig covering the entire region of Chlorella vulgaris chromosome I (980 kb long), consisting of 33 cosmid clones has been constructed. By cross-hybridization with other chromosomal DNAs, universal structural elements were detected and localized on the contig. They were composed of at least three different elements: short interspersed DNA elements (SINE)-like elements, long interspersed DNA elements (LINE)-like elements and a putative centromere-like element. At least 36 copies of SINE-like elements were distributed over chromosome I with preferential locations on the right half of the chromosome. DNA fragments containing a SINE-like sequence showed a bent or curved DNA nature on polyacrylamide gel electrophoresis. LINE-like elements were clustered at the left terminus of chromosome I where they formed a tandem array of six copies immediately adjacent to the telomeric repeats. A long sequence element localized at a unique region of chromosome I also existed in a single copy on each chromosome and contained a sequence related to the reverse transcriptase domain of retrotransposons. This feature was compared with the reported centromere-associated elements of higher plants. With its comparative simplicity, the organization of Chlorella chromosome I genomic elements may serve as a prototypic experimental system for deciphering the complexity of huge plant chromosomes.

Original languageEnglish
Pages (from-to)3900-3907
Number of pages8
JournalNucleic acids research.
Volume26
Issue number17
Publication statusPublished - Sep 1 1998
Externally publishedYes

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Chlorella vulgaris
Chlorophyta
Anatomy
Chromosomes
DNA
Centromere
Plant Chromosomes
Short Interspersed Nucleotide Elements
Chlorella
Cosmids
Retroelements
RNA-Directed DNA Polymerase
Polyacrylamide Gel Electrophoresis
Clone Cells

ASJC Scopus subject areas

  • Genetics

Cite this

Noutoshi, Y., Ito, Y., Kanetani, S., Fujie, M., Usami, S., & Yamada, T. (1998). Molecular anatomy of a small chromosome in the green alga Chlorella vulgaris. Nucleic acids research., 26(17), 3900-3907.

Molecular anatomy of a small chromosome in the green alga Chlorella vulgaris. / Noutoshi, Yoshiteru; Ito, Yukari; Kanetani, Shinsaku; Fujie, Makoto; Usami, Shouji; Yamada, Takashi.

In: Nucleic acids research., Vol. 26, No. 17, 01.09.1998, p. 3900-3907.

Research output: Contribution to journalArticle

Noutoshi, Y, Ito, Y, Kanetani, S, Fujie, M, Usami, S & Yamada, T 1998, 'Molecular anatomy of a small chromosome in the green alga Chlorella vulgaris', Nucleic acids research., vol. 26, no. 17, pp. 3900-3907.
Noutoshi Y, Ito Y, Kanetani S, Fujie M, Usami S, Yamada T. Molecular anatomy of a small chromosome in the green alga Chlorella vulgaris. Nucleic acids research. 1998 Sep 1;26(17):3900-3907.
Noutoshi, Yoshiteru ; Ito, Yukari ; Kanetani, Shinsaku ; Fujie, Makoto ; Usami, Shouji ; Yamada, Takashi. / Molecular anatomy of a small chromosome in the green alga Chlorella vulgaris. In: Nucleic acids research. 1998 ; Vol. 26, No. 17. pp. 3900-3907.
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