Molecular analysis of rice dwarf phytoreovirus segment S11 corresponding to wound tumour phytoreovirus segment S12

Nobuhiro Suzuki, M. Harada, T. Kusano

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The complete nucleotide sequence of rice dwarf phytoreovirus (RDV) genome segment S11 was determined. S11 is 1067 nucleotides long. There is an inverted repeat of 10 bp adjacent to the conserved 5'terminal hexanucleotide (5' GGUAAA 3') and 3'terminal tetranucleotide (5' UAGU 3') sequences. A single large open reading frame found in the plus strand of S11 begins with the first AUG codon (bases 6 to 8) and extends for 567 bases. Evolutionary relatedness between RDV S11 and wound tumour phytoreovirus S12 based on amino acid sequence similarity (25-8%) was found. In addition to the first AUG triplet, RDV S11 possesses a second in-phase AUG triplet (positions 30 to 32) nearby, which conforms to the Kozak consensus sequence. Two forms of the protein were identified by using an in vitro transcription and translation system in which a tailored full-length cDNA was the initial template. The abolition of the first AUG codon by site-directed mutagenesis resulted in disappearance of the larger translation product. These results strongly suggest that the two products are translated from the first and second AUG codons. Whether the two proteins are expressed in vivo is at present unclear.

Original languageEnglish
Pages (from-to)2233-2237
Number of pages5
JournalJournal of General Virology
Volume72
Issue number9
Publication statusPublished - 1991
Externally publishedYes

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Reoviridae
Codon
Wounds and Injuries
Neoplasms
Consensus Sequence
Site-Directed Mutagenesis
Open Reading Frames
Amino Acid Sequence
Proteins
Nucleotides
Complementary DNA
Genome
Oryza

ASJC Scopus subject areas

  • Virology
  • Immunology

Cite this

Molecular analysis of rice dwarf phytoreovirus segment S11 corresponding to wound tumour phytoreovirus segment S12. / Suzuki, Nobuhiro; Harada, M.; Kusano, T.

In: Journal of General Virology, Vol. 72, No. 9, 1991, p. 2233-2237.

Research output: Contribution to journalArticle

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