Molecular analysis of a new insertion sequence from Actinobacillus (Haemophilus) actinomycetemcomitans FDC Y4

Hideaki Hayashida, Hitoshi Hotokezaka, Naoya Ohara, Makoto Kimura, Okiuji Takagi, Takeshi Yamada

Research output: Contribution to journalArticle

10 Citations (Scopus)


We have found a new insertion sequence (IS), designated ISAa1, downstream of the S10 operon in Actinobacillus (Haemophilus) actinomycetemcomitans FDC Y4. ISAa1, the first IS element characterized in this organism, is 705 bp long and lacks terminal inverted repeats. This element displayed significant homology with IS200. Hybridization patterns of genomic DNA of seven A. actinomycetemcomitans strains with an internal ISAa1 probe varied depending on the serotypes, suggesting that ISAa1 might be a useful tool for epidemiological studies.

Original languageEnglish
Pages (from-to)2449-2452
Number of pages4
Issue number9
Publication statusPublished - Sep 1996



  • Actinobacillus actinomycetemcomitans
  • ISAa1

ASJC Scopus subject areas

  • Microbiology

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