TY - JOUR
T1 - Modulation of the intermolecular interaction of myoglobin by removal of the heme
AU - Imamura, Hiroshi
AU - Morita, Takeshi
AU - Sumi, Tomonari
AU - Isogai, Yasuhiro
AU - Kato, Minoru
AU - Nishikawa, Keiko
PY - 2013/11
Y1 - 2013/11
N2 - Toward understanding intermolecular interactions governing self-association of proteins, the present study investigated a model protein, myoglobin, using a small-angle X-ray scattering technique. It has been known that removal of the heme makes myoglobin aggregation-prone. The interparticle interferences of the holomyoglobin and the apomyoglobin were compared in terms of the structure factor. Analysis of the structure factor using a model potential of Derjaguin-Laudau-Verwey-Overbeek (DLVO) suggests that the intermolecular interaction potential of apomyoglobin is more attractive than that of holomyoglobin at short range from the protein molecule.
AB - Toward understanding intermolecular interactions governing self-association of proteins, the present study investigated a model protein, myoglobin, using a small-angle X-ray scattering technique. It has been known that removal of the heme makes myoglobin aggregation-prone. The interparticle interferences of the holomyoglobin and the apomyoglobin were compared in terms of the structure factor. Analysis of the structure factor using a model potential of Derjaguin-Laudau-Verwey-Overbeek (DLVO) suggests that the intermolecular interaction potential of apomyoglobin is more attractive than that of holomyoglobin at short range from the protein molecule.
KW - protein engineering
KW - protein-protein interaction
KW - small-angle X-ray scattering
UR - http://www.scopus.com/inward/record.url?scp=84885622275&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84885622275&partnerID=8YFLogxK
U2 - 10.1107/S0909049513022772
DO - 10.1107/S0909049513022772
M3 - Article
C2 - 24121340
AN - SCOPUS:84885622275
SN - 0909-0495
VL - 20
SP - 919
EP - 922
JO - Journal of Synchrotron Radiation
JF - Journal of Synchrotron Radiation
IS - 6
ER -