Modulation of neutrophil influx in glomerulonephritis in the rat with anti-macrophage inflammatory protein-2 (MIP-2) antibody

L. Feng, Y. Xia, Teizo Yoshimura, C. B. Wilson

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162 Citations (Scopus)

Abstract

The role of the chemokine, macrophage inflammatory protein-2 (MIP-2), during anti-glomerular basement membrane (GBM) antibody (Ab) glomerulonephritis (GN) was studied. Rat MIP-2 cDNA had been cloned previously. Recombinant rat MIP-2 (rMIP-2) from Escherichia coli exhibited neutrophil chemotactic activity and produced neutrophil influx when injected into the rat bladder wall. By using a riboprobe derived from the cDNA and an anti-rMIP-2 polyclonal Ab, MIP-2 was found to be induced in glomeruli with anti-GBM Ab GN as mRNA by 30 min and protein by 4 h, with both disappearing by 24 h. The expression of MIP-2 correlated with glomerular neutrophil influx. A single dose of the anti-MIP-2 Ab 30 min before anti-GBM Ab was effective in reducing neutrophil influx (40% at 4 h, P <0.01) and periodic acid-Schiff deposits containing fibrin (54% at 24 h, P <0.01). The anti- rMIP-2 Ab had no effect on anti-GBM Ab binding (paired-label isotope study). Functional improvement in the glomerular damage was evidenced by a reduction of abnormal proteinuria (P <0.05). These results suggest that MIP-2 is a major neutrophil chemoattractant contributing to influx of neutrophils in Ab- induced glomerular inflammation in the rat.

Original languageEnglish
Pages (from-to)1009-1017
Number of pages9
JournalJournal of Clinical Investigation
Volume95
Issue number3
Publication statusPublished - 1995
Externally publishedYes

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Chemokine CXCL2
Glomerulonephritis
Neutrophils
Anti-Inflammatory Agents
Glomerular Basement Membrane
Antibodies
Complementary DNA
Periodic Acid
Chemotactic Factors
Fibrin
Proteinuria
Chemokines
Isotopes
Urinary Bladder
Escherichia coli
Inflammation
Messenger RNA

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Modulation of neutrophil influx in glomerulonephritis in the rat with anti-macrophage inflammatory protein-2 (MIP-2) antibody. / Feng, L.; Xia, Y.; Yoshimura, Teizo; Wilson, C. B.

In: Journal of Clinical Investigation, Vol. 95, No. 3, 1995, p. 1009-1017.

Research output: Contribution to journalArticle

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