Modulation of endothelin-induced intracellular Ca2+ mobilization by interleukin-1β and lipopolysaccharide in C6 rat glioma cells

A. Kugaya, A. Kagaya, H. Zensho, T. Oyamada, Y. Tawara, M. Inagaki, Y. Uchitomi, S. Yamawaki

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7 Citations (Scopus)


We have investigated the effects of interleukin (IL)-(IL)-1̄ and lipopolysaccharide (LPS) on endothelin (ET)-induced intracellular Ca2+ rise in C6 rat glioma cells in order to study the mechanisms of their effects on Ca2+ signaling systems. Pretreatment with IL-1̄ (103 U/mL) and LPS (1 μg/mL) for 24 h significantly inhibited 100 nM ET-l-induced increase in intracellular Ca2+ either in the presence or absence of external Ca2+. Their inhibitory effects were in dose-dependent (IL-1β; 50-1000 U/mL, LPS; 10-1000 ng/mL) and time-dependent (12-24 h) manners. A tyrosine kinase antagonist genistein (50 μM) but not a protein kinase C inhibitor H7 (30 μM) prevented the inhibition of the ET response by IL-1β and LPS. These results suggest that activation of tyrosine kinase may be essential for the inhibition of the ET receptor-mediated Ca2+ signaling systems by IL-1β and LPS.

Original languageEnglish
Pages (from-to)187-192
Number of pages6
Issue number2
Publication statusPublished - 1997

ASJC Scopus subject areas

  • Endocrinology
  • Neurology
  • Endocrine and Autonomic Systems
  • Cellular and Molecular Neuroscience


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