Modulation of endothelin-induced intracellular Ca2+ mobilization by interleukin-1β and lipopolysaccharide in C6 rat glioma cells

A. Kugaya, A. Kagaya, H. Zensho, T. Oyamada, Y. Tawara, M. Inagaki, Y. Uchitomi, S. Yamawaki

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

We have investigated the effects of interleukin (IL)-(IL)-1̄ and lipopolysaccharide (LPS) on endothelin (ET)-induced intracellular Ca2+ rise in C6 rat glioma cells in order to study the mechanisms of their effects on Ca2+ signaling systems. Pretreatment with IL-1̄ (103 U/mL) and LPS (1 μg/mL) for 24 h significantly inhibited 100 nM ET-l-induced increase in intracellular Ca2+ either in the presence or absence of external Ca2+. Their inhibitory effects were in dose-dependent (IL-1β; 50-1000 U/mL, LPS; 10-1000 ng/mL) and time-dependent (12-24 h) manners. A tyrosine kinase antagonist genistein (50 μM) but not a protein kinase C inhibitor H7 (30 μM) prevented the inhibition of the ET response by IL-1β and LPS. These results suggest that activation of tyrosine kinase may be essential for the inhibition of the ET receptor-mediated Ca2+ signaling systems by IL-1β and LPS.

Original languageEnglish
Pages (from-to)187-192
Number of pages6
JournalNeuropeptides
Volume31
Issue number2
DOIs
Publication statusPublished - 1997
Externally publishedYes

Fingerprint

Endothelins
Interleukin-1
Glioma
Lipopolysaccharides
Rats
Modulation
Protein-Tyrosine Kinases
Endothelin Receptors
Protein C Inhibitor
Genistein
Interleukins
Protein Kinase Inhibitors
Protein Kinase C
Chemical activation

ASJC Scopus subject areas

  • Endocrinology
  • Clinical Neurology
  • Endocrinology, Diabetes and Metabolism
  • Cellular and Molecular Neuroscience
  • Neuroscience(all)
  • Biochemistry

Cite this

Modulation of endothelin-induced intracellular Ca2+ mobilization by interleukin-1β and lipopolysaccharide in C6 rat glioma cells. / Kugaya, A.; Kagaya, A.; Zensho, H.; Oyamada, T.; Tawara, Y.; Inagaki, M.; Uchitomi, Y.; Yamawaki, S.

In: Neuropeptides, Vol. 31, No. 2, 1997, p. 187-192.

Research output: Contribution to journalArticle

Kugaya, A, Kagaya, A, Zensho, H, Oyamada, T, Tawara, Y, Inagaki, M, Uchitomi, Y & Yamawaki, S 1997, 'Modulation of endothelin-induced intracellular Ca2+ mobilization by interleukin-1β and lipopolysaccharide in C6 rat glioma cells', Neuropeptides, vol. 31, no. 2, pp. 187-192. https://doi.org/10.1016/S0143-4179(97)90088-3
Kugaya, A. ; Kagaya, A. ; Zensho, H. ; Oyamada, T. ; Tawara, Y. ; Inagaki, M. ; Uchitomi, Y. ; Yamawaki, S. / Modulation of endothelin-induced intracellular Ca2+ mobilization by interleukin-1β and lipopolysaccharide in C6 rat glioma cells. In: Neuropeptides. 1997 ; Vol. 31, No. 2. pp. 187-192.
@article{111c058e215b400986ce4087a0b9762c,
title = "Modulation of endothelin-induced intracellular Ca2+ mobilization by interleukin-1β and lipopolysaccharide in C6 rat glioma cells",
abstract = "We have investigated the effects of interleukin (IL)-(IL)-1̄ and lipopolysaccharide (LPS) on endothelin (ET)-induced intracellular Ca2+ rise in C6 rat glioma cells in order to study the mechanisms of their effects on Ca2+ signaling systems. Pretreatment with IL-1̄ (103 U/mL) and LPS (1 μg/mL) for 24 h significantly inhibited 100 nM ET-l-induced increase in intracellular Ca2+ either in the presence or absence of external Ca2+. Their inhibitory effects were in dose-dependent (IL-1β; 50-1000 U/mL, LPS; 10-1000 ng/mL) and time-dependent (12-24 h) manners. A tyrosine kinase antagonist genistein (50 μM) but not a protein kinase C inhibitor H7 (30 μM) prevented the inhibition of the ET response by IL-1β and LPS. These results suggest that activation of tyrosine kinase may be essential for the inhibition of the ET receptor-mediated Ca2+ signaling systems by IL-1β and LPS.",
author = "A. Kugaya and A. Kagaya and H. Zensho and T. Oyamada and Y. Tawara and M. Inagaki and Y. Uchitomi and S. Yamawaki",
year = "1997",
doi = "10.1016/S0143-4179(97)90088-3",
language = "English",
volume = "31",
pages = "187--192",
journal = "Neuropeptides",
issn = "0143-4179",
publisher = "Churchill Livingstone",
number = "2",

}

TY - JOUR

T1 - Modulation of endothelin-induced intracellular Ca2+ mobilization by interleukin-1β and lipopolysaccharide in C6 rat glioma cells

AU - Kugaya, A.

AU - Kagaya, A.

AU - Zensho, H.

AU - Oyamada, T.

AU - Tawara, Y.

AU - Inagaki, M.

AU - Uchitomi, Y.

AU - Yamawaki, S.

PY - 1997

Y1 - 1997

N2 - We have investigated the effects of interleukin (IL)-(IL)-1̄ and lipopolysaccharide (LPS) on endothelin (ET)-induced intracellular Ca2+ rise in C6 rat glioma cells in order to study the mechanisms of their effects on Ca2+ signaling systems. Pretreatment with IL-1̄ (103 U/mL) and LPS (1 μg/mL) for 24 h significantly inhibited 100 nM ET-l-induced increase in intracellular Ca2+ either in the presence or absence of external Ca2+. Their inhibitory effects were in dose-dependent (IL-1β; 50-1000 U/mL, LPS; 10-1000 ng/mL) and time-dependent (12-24 h) manners. A tyrosine kinase antagonist genistein (50 μM) but not a protein kinase C inhibitor H7 (30 μM) prevented the inhibition of the ET response by IL-1β and LPS. These results suggest that activation of tyrosine kinase may be essential for the inhibition of the ET receptor-mediated Ca2+ signaling systems by IL-1β and LPS.

AB - We have investigated the effects of interleukin (IL)-(IL)-1̄ and lipopolysaccharide (LPS) on endothelin (ET)-induced intracellular Ca2+ rise in C6 rat glioma cells in order to study the mechanisms of their effects on Ca2+ signaling systems. Pretreatment with IL-1̄ (103 U/mL) and LPS (1 μg/mL) for 24 h significantly inhibited 100 nM ET-l-induced increase in intracellular Ca2+ either in the presence or absence of external Ca2+. Their inhibitory effects were in dose-dependent (IL-1β; 50-1000 U/mL, LPS; 10-1000 ng/mL) and time-dependent (12-24 h) manners. A tyrosine kinase antagonist genistein (50 μM) but not a protein kinase C inhibitor H7 (30 μM) prevented the inhibition of the ET response by IL-1β and LPS. These results suggest that activation of tyrosine kinase may be essential for the inhibition of the ET receptor-mediated Ca2+ signaling systems by IL-1β and LPS.

UR - http://www.scopus.com/inward/record.url?scp=0030953609&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030953609&partnerID=8YFLogxK

U2 - 10.1016/S0143-4179(97)90088-3

DO - 10.1016/S0143-4179(97)90088-3

M3 - Article

C2 - 9179872

AN - SCOPUS:0030953609

VL - 31

SP - 187

EP - 192

JO - Neuropeptides

JF - Neuropeptides

SN - 0143-4179

IS - 2

ER -