Modulation of cell surface lectin receptors on K562 human erythroleukemia cells induced by transfection with annexin IV cDNA

Ayano Satoh; Eiji Takayama; Kyoko Kojima; Haruko Ogawa; Yoshimoto Katsura; Tatsuo Kina; Tatsuro Irimura; Isamu Matsumoto

doi:10.1016/S0014-5793(97)00161-0

Modulation of cell surface lectin receptors on K562 human erythroleukemia cells induced by transfection with annexin IV cDNA

Ayano Satoh, Eiji Takayama, Kyoko Kojima, Haruko Ogawa, Yoshimoto Katsura, Tatsuo Kina, Tatsuro Irimura, Isamu Matsumoto

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

Annexin IV was found to be highly expressed in various human adenocarcinoma cell lines, but not in an erythroleukemia cell line, K562. We investigated the effects of transfection of human annexin IV cDNA into K562 cells on cell surface lectin receptors. cDNA transfectants were found to he more sensitive to cytotoxic lectins such as Ricinus communis agglutinin and wheat germ agglutinin than mock transfectants. The results of flow cytometric analyses with various lectins showed that the transfectants expressed more sugar chains which bind to Ulex europaeus agglutinin I and Maackia amurensis mitogen than mock transfectants. These results suggest that transfection of annexin IV cDNA increases the expression of α-2,3-sialylated and/or fucosylated sugar chains on the surface.

Original language	English
Pages (from-to)	107-110
Number of pages	4
Journal	FEBS Letters
Volume	405
Issue number	1
DOIs	https://doi.org/10.1016/S0014-5793(97)00161-0
Publication status	Published - Mar 17 1997
Externally published	Yes

Keywords

Annexin IV
Endocytosis
K562
Lectin
Transfection

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/S0014-5793(97)00161-0

Cite this

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title = "Modulation of cell surface lectin receptors on K562 human erythroleukemia cells induced by transfection with annexin IV cDNA",

abstract = "Annexin IV was found to be highly expressed in various human adenocarcinoma cell lines, but not in an erythroleukemia cell line, K562. We investigated the effects of transfection of human annexin IV cDNA into K562 cells on cell surface lectin receptors. cDNA transfectants were found to he more sensitive to cytotoxic lectins such as Ricinus communis agglutinin and wheat germ agglutinin than mock transfectants. The results of flow cytometric analyses with various lectins showed that the transfectants expressed more sugar chains which bind to Ulex europaeus agglutinin I and Maackia amurensis mitogen than mock transfectants. These results suggest that transfection of annexin IV cDNA increases the expression of α-2,3-sialylated and/or fucosylated sugar chains on the surface.",

keywords = "Annexin IV, Endocytosis, K562, Lectin, Transfection",

author = "Ayano Satoh and Eiji Takayama and Kyoko Kojima and Haruko Ogawa and Yoshimoto Katsura and Tatsuo Kina and Tatsuro Irimura and Isamu Matsumoto",

note = "Funding Information: This work was supported by the Hayashi Memorial Foundation for Female Natural Scientists.",

year = "1997",

month = mar,

day = "17",

doi = "10.1016/S0014-5793(97)00161-0",

language = "English",

volume = "405",

pages = "107--110",

journal = "FEBS Letters",

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TY - JOUR

T1 - Modulation of cell surface lectin receptors on K562 human erythroleukemia cells induced by transfection with annexin IV cDNA

AU - Satoh, Ayano

AU - Takayama, Eiji

AU - Kojima, Kyoko

AU - Ogawa, Haruko

AU - Katsura, Yoshimoto

AU - Kina, Tatsuo

AU - Irimura, Tatsuro

AU - Matsumoto, Isamu

N1 - Funding Information: This work was supported by the Hayashi Memorial Foundation for Female Natural Scientists.

PY - 1997/3/17

Y1 - 1997/3/17

N2 - Annexin IV was found to be highly expressed in various human adenocarcinoma cell lines, but not in an erythroleukemia cell line, K562. We investigated the effects of transfection of human annexin IV cDNA into K562 cells on cell surface lectin receptors. cDNA transfectants were found to he more sensitive to cytotoxic lectins such as Ricinus communis agglutinin and wheat germ agglutinin than mock transfectants. The results of flow cytometric analyses with various lectins showed that the transfectants expressed more sugar chains which bind to Ulex europaeus agglutinin I and Maackia amurensis mitogen than mock transfectants. These results suggest that transfection of annexin IV cDNA increases the expression of α-2,3-sialylated and/or fucosylated sugar chains on the surface.

AB - Annexin IV was found to be highly expressed in various human adenocarcinoma cell lines, but not in an erythroleukemia cell line, K562. We investigated the effects of transfection of human annexin IV cDNA into K562 cells on cell surface lectin receptors. cDNA transfectants were found to he more sensitive to cytotoxic lectins such as Ricinus communis agglutinin and wheat germ agglutinin than mock transfectants. The results of flow cytometric analyses with various lectins showed that the transfectants expressed more sugar chains which bind to Ulex europaeus agglutinin I and Maackia amurensis mitogen than mock transfectants. These results suggest that transfection of annexin IV cDNA increases the expression of α-2,3-sialylated and/or fucosylated sugar chains on the surface.

KW - Annexin IV

KW - Endocytosis

KW - K562

KW - Lectin

KW - Transfection

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U2 - 10.1016/S0014-5793(97)00161-0

DO - 10.1016/S0014-5793(97)00161-0

M3 - Article

C2 - 9094435

AN - SCOPUS:0030945407

VL - 405

SP - 107

EP - 110

JO - FEBS Letters

JF - FEBS Letters

SN - 0014-5793

IS - 1

ER -

Modulation of cell surface lectin receptors on K562 human erythroleukemia cells induced by transfection with annexin IV cDNA

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

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