Abstract
Annexin IV was found to be highly expressed in various human adenocarcinoma cell lines, but not in an erythroleukemia cell line, K562. We investigated the effects of transfection of human annexin IV cDNA into K562 cells on cell surface lectin receptors. cDNA transfectants were found to he more sensitive to cytotoxic lectins such as Ricinus communis agglutinin and wheat germ agglutinin than mock transfectants. The results of flow cytometric analyses with various lectins showed that the transfectants expressed more sugar chains which bind to Ulex europaeus agglutinin I and Maackia amurensis mitogen than mock transfectants. These results suggest that transfection of annexin IV cDNA increases the expression of α-2,3-sialylated and/or fucosylated sugar chains on the surface.
| Original language | English |
|---|---|
| Pages (from-to) | 107-110 |
| Number of pages | 4 |
| Journal | FEBS Letters |
| Volume | 405 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Mar 17 1997 |
| Externally published | Yes |
Fingerprint
Keywords
- Annexin IV
- Endocytosis
- K562
- Lectin
- Transfection
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
Cite this
Modulation of cell surface lectin receptors on K562 human erythroleukemia cells induced by transfection with annexin IV cDNA. / Satoh, Ayano; Takayama, Eiji; Kojima, Kyoko; Ogawa, Haruko; Katsura, Yoshimoto; Kina, Tatsuo; Irimura, Tatsuro; Matsumoto, Isamu.
In: FEBS Letters, Vol. 405, No. 1, 17.03.1997, p. 107-110.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Modulation of cell surface lectin receptors on K562 human erythroleukemia cells induced by transfection with annexin IV cDNA
AU - Satoh, Ayano
AU - Takayama, Eiji
AU - Kojima, Kyoko
AU - Ogawa, Haruko
AU - Katsura, Yoshimoto
AU - Kina, Tatsuo
AU - Irimura, Tatsuro
AU - Matsumoto, Isamu
PY - 1997/3/17
Y1 - 1997/3/17
N2 - Annexin IV was found to be highly expressed in various human adenocarcinoma cell lines, but not in an erythroleukemia cell line, K562. We investigated the effects of transfection of human annexin IV cDNA into K562 cells on cell surface lectin receptors. cDNA transfectants were found to he more sensitive to cytotoxic lectins such as Ricinus communis agglutinin and wheat germ agglutinin than mock transfectants. The results of flow cytometric analyses with various lectins showed that the transfectants expressed more sugar chains which bind to Ulex europaeus agglutinin I and Maackia amurensis mitogen than mock transfectants. These results suggest that transfection of annexin IV cDNA increases the expression of α-2,3-sialylated and/or fucosylated sugar chains on the surface.
AB - Annexin IV was found to be highly expressed in various human adenocarcinoma cell lines, but not in an erythroleukemia cell line, K562. We investigated the effects of transfection of human annexin IV cDNA into K562 cells on cell surface lectin receptors. cDNA transfectants were found to he more sensitive to cytotoxic lectins such as Ricinus communis agglutinin and wheat germ agglutinin than mock transfectants. The results of flow cytometric analyses with various lectins showed that the transfectants expressed more sugar chains which bind to Ulex europaeus agglutinin I and Maackia amurensis mitogen than mock transfectants. These results suggest that transfection of annexin IV cDNA increases the expression of α-2,3-sialylated and/or fucosylated sugar chains on the surface.
KW - Annexin IV
KW - Endocytosis
KW - K562
KW - Lectin
KW - Transfection
UR - http://www.scopus.com/inward/record.url?scp=0030945407&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030945407&partnerID=8YFLogxK
U2 - 10.1016/S0014-5793(97)00161-0
DO - 10.1016/S0014-5793(97)00161-0
M3 - Article
C2 - 9094435
AN - SCOPUS:0030945407
VL - 405
SP - 107
EP - 110
JO - FEBS Letters
JF - FEBS Letters
SN - 0014-5793
IS - 1
ER -