Modulation of cell surface lectin receptors on K562 human erythroleukemia cells induced by transfection with annexin IV cDNA

Ayano Satoh, Eiji Takayama, Kyoko Kojima, Haruko Ogawa, Yoshimoto Katsura, Tatsuo Kina, Tatsuro Irimura, Isamu Matsumoto

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Annexin IV was found to be highly expressed in various human adenocarcinoma cell lines, but not in an erythroleukemia cell line, K562. We investigated the effects of transfection of human annexin IV cDNA into K562 cells on cell surface lectin receptors. cDNA transfectants were found to he more sensitive to cytotoxic lectins such as Ricinus communis agglutinin and wheat germ agglutinin than mock transfectants. The results of flow cytometric analyses with various lectins showed that the transfectants expressed more sugar chains which bind to Ulex europaeus agglutinin I and Maackia amurensis mitogen than mock transfectants. These results suggest that transfection of annexin IV cDNA increases the expression of α-2,3-sialylated and/or fucosylated sugar chains on the surface.

Original languageEnglish
Pages (from-to)107-110
Number of pages4
JournalFEBS Letters
Volume405
Issue number1
DOIs
Publication statusPublished - Mar 17 1997
Externally publishedYes

Fingerprint

Annexin A4
Mitogen Receptors
Leukemia, Erythroblastic, Acute
Cell Surface Receptors
Transfection
Complementary DNA
Modulation
Lectins
Sugars
Maackia
Cells
Ulex
Cell Line
Wheat Germ Agglutinins
K562 Cells
Agglutinins
Mitogens
Adenocarcinoma

Keywords

  • Annexin IV
  • Endocytosis
  • K562
  • Lectin
  • Transfection

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Modulation of cell surface lectin receptors on K562 human erythroleukemia cells induced by transfection with annexin IV cDNA. / Satoh, Ayano; Takayama, Eiji; Kojima, Kyoko; Ogawa, Haruko; Katsura, Yoshimoto; Kina, Tatsuo; Irimura, Tatsuro; Matsumoto, Isamu.

In: FEBS Letters, Vol. 405, No. 1, 17.03.1997, p. 107-110.

Research output: Contribution to journalArticle

Satoh, Ayano ; Takayama, Eiji ; Kojima, Kyoko ; Ogawa, Haruko ; Katsura, Yoshimoto ; Kina, Tatsuo ; Irimura, Tatsuro ; Matsumoto, Isamu. / Modulation of cell surface lectin receptors on K562 human erythroleukemia cells induced by transfection with annexin IV cDNA. In: FEBS Letters. 1997 ; Vol. 405, No. 1. pp. 107-110.
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AU - Irimura, Tatsuro

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