Modulation of AP-1 activity by nitric oxide (NO) in vitro: NO-mediated modulation of AP-1

Akiko Tabuchi, Kuniaki Sano, Esther Oh, Tomofusa Tsuchiya, Masaaki Tsuda

Research output: Contribution to journalArticle

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Abstract

To understand the role of nitric oxide (NO) in controlling the specific DNA-binding activities of transcriptional factors, we investigated the in vitro effect of the NO-donor sodium nitroprusside (SNP) on the AP-1 activity of cultured mouse cerebellar granule cells. A gel-mobility assay showed that SNP inhibited AP-1 activity in the presence, but not the absence of dithiothreitol (DTT). This DTT-dependent inhibition of AP-1 activity by SNP corresponded with the activation of the chemical reactivity of SNP with DTT, which can be monitored by the production of nitrite (NO-2). In contrast, diamide, a typical sulfhydryl oxidizing agent, inhibited AP-1 activity in the absence of DTT and its inhibitory effect was reversed competitively by DTT. Studies using structurally or functionally related analogues of SNP demonstrated that S-nitrosylation of the AP-1 moiety mediated by some NO-carriers but not by free NO, which can be produced by the chemical reaction of SNP with DTT, was responsible for the inhibition of AP-1 activity, suggesting NO-mediated regulation of the AP-1 transcriptional factor.

Original languageEnglish
Pages (from-to)123-127
Number of pages5
JournalFEBS Letters
Volume351
Issue number1
DOIs
Publication statusPublished - Aug 29 1994

Fingerprint

Transcription Factor AP-1
Dithiothreitol
Nitroprusside
Nitric Oxide
Modulation
Diamide
Chemical reactivity
Nitric Oxide Donors
Nitrites
In Vitro Techniques
Oxidants
Chemical reactions
Assays
Gels
Chemical activation
DNA

Keywords

  • Nitric oxide (NO) AP-1
  • Redox regulation
  • S-nitrosylation
  • Sodium nitroprusside (SNP)
  • Transcriptional factor

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Modulation of AP-1 activity by nitric oxide (NO) in vitro : NO-mediated modulation of AP-1. / Tabuchi, Akiko; Sano, Kuniaki; Oh, Esther; Tsuchiya, Tomofusa; Tsuda, Masaaki.

In: FEBS Letters, Vol. 351, No. 1, 29.08.1994, p. 123-127.

Research output: Contribution to journalArticle

Tabuchi, Akiko ; Sano, Kuniaki ; Oh, Esther ; Tsuchiya, Tomofusa ; Tsuda, Masaaki. / Modulation of AP-1 activity by nitric oxide (NO) in vitro : NO-mediated modulation of AP-1. In: FEBS Letters. 1994 ; Vol. 351, No. 1. pp. 123-127.
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