Modification at position 9 with 1-methyladenosine is crucial for structure and function of nematode mitochondrial tRNAs lacking the entire T-arm

Masayuki Sakurai, Takashi Ohtsuki, Kimitsuna Watanabe

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

The mitochondria of the nematode Ascaris suum have tRNAs with unusual secondary structures that lack either the T-arm or D-arm found in most other organisms. Of the twenty-two tRNA species present in the mitochondria of A.suum, twenty lack the entire T-arm and two serine tRNAs lack the D-arm. To understand how such unusual tRNAs work in the nematode mitochondrial translation system, we analyzed post-transcriptional modifications of 11 mitochondrial tRNA species purified from A.suum, 10 of which lacked a T-arm and one of which lacked a D-arm. The most characteristic feature of nematode mitochondrial tRNAs lacking a T-arm was the presence of 1-methyladenosine at position 9 (m1A9). Synthesis of T-armless tRNAs with or without the modified nucleoside showed that T-armless tRNAs without the modification had much lower aminoacylation and EF-Tu-binding activities than native tRNAs. The addition of a single methyl group to A9 of these tRNAs was sufficient to restore nearly native levels of aminoacylation and EF-Tu-binding activity as well as tertiary structure, suggesting that m1A9 is a key residue for the activity of T-armless tRNAs. Thus, m1A9 is indispensable for the structure and function of T-armless tRNAs of nematode mitochondrial origin.

Original languageEnglish
Pages (from-to)1653-1661
Number of pages9
JournalNucleic acids research.
Volume33
Issue number5
DOIs
Publication statusPublished - 2005

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Transfer RNA
Peptide Elongation Factor Tu
Aminoacylation
1-methyladenosine
Mitochondria
Ascaris suum
Nucleosides
Serine

ASJC Scopus subject areas

  • Genetics

Cite this

Modification at position 9 with 1-methyladenosine is crucial for structure and function of nematode mitochondrial tRNAs lacking the entire T-arm. / Sakurai, Masayuki; Ohtsuki, Takashi; Watanabe, Kimitsuna.

In: Nucleic acids research., Vol. 33, No. 5, 2005, p. 1653-1661.

Research output: Contribution to journalArticle

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