Model-potential-free analysis of small angle scattering of proteins in solution: Insights into solvent effects on protein-protein interaction

Tomonari Sumi, Hiroshi Imamura, Takeshi Morita, Yasuhiro Isogai, Keiko Nishikawa

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6 Citations (Scopus)

Abstract

To extract protein-protein interaction from experimental small-angle scattering of proteins in solutions using liquid state theory, a model potential consisting of a hard-sphere repulsive potential and the excess interaction potential has been introduced. In the present study, we propose a model-potential-free integral equation method that extracts the excess interaction potential by using the experimental small-angle scattering data without specific model potential such as the Derjaguin-Landau-Verwey-Overbeek (DLVO)-type model. Our analysis of experimental small-angle X-ray scattering data for lysozyme solution shows both the stabilization of contact configurations of protein molecules and a large activation barrier against the formation of the contact configurations in addition to the screened Coulomb repulsion. These characteristic features, which are not well-described by the DLVO-type model, are interpreted as solvent effects. This journal is

Original languageEnglish
Pages (from-to)25492-25497
Number of pages6
JournalPhysical Chemistry Chemical Physics
Volume16
Issue number46
DOIs
Publication statusPublished - Dec 14 2014

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ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Physics and Astronomy(all)
  • Medicine(all)

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