Mobilization of a transposon in the rice genome

Tetsuya Nakazaki, Yutaka Okumoto, Akira Horibata, Satoshi Yamahira, Masayoshi Teraishi, Hidetaka Nishida, Hiromo Inoue, Takatoshi Tanisaka

Research output: Contribution to journalArticle

183 Citations (Scopus)

Abstract

Rice (Oryza sativa L.) is an important crop worldwide and, with the availability of the draft sequence1,2, a useful model for analysing the genome structure of grasses3,4. To practice efficient rice breeding through genetic engineering techniques, it is important to identify the economically important genes in this crop. The use of mobile transposons as gene tags in intact plants is a powerful tool for functional analysis because transposon insertions often inactivate genes5. Here we identify an active rice transposon named miniature Ping (mPing) through analysis of the mutability of a slender mutation of the glume6 - the seed structure that encloses and determines the shape of the grain. The mPing transposon is inserted in the slender glume (slg) mutant allele but not in the wild-type allele. Search of the O. sativa variety Nipponbare genome identified 34 sequences with high nucleotide similarity to mPing, indicating that mPing constitutes a family of transposon elements. Excision of mPing from slg plants results in reversion to a wild-type phenotype. The mobility of the transposon mPing in intact rice plants represents a useful alternative tool for the functional analysis of rice genes.

Original languageEnglish
Pages (from-to)170-172
Number of pages3
JournalNature
Volume421
Issue number6919
DOIs
Publication statusPublished - Jan 9 2003
Externally publishedYes

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Genome
Alleles
Genes
Genetic Techniques
Genetic Engineering
Oryza
Breeding
Seeds
Nucleotides
Phenotype
Mutation

ASJC Scopus subject areas

  • General

Cite this

Nakazaki, T., Okumoto, Y., Horibata, A., Yamahira, S., Teraishi, M., Nishida, H., ... Tanisaka, T. (2003). Mobilization of a transposon in the rice genome. Nature, 421(6919), 170-172. https://doi.org/10.1038/nature01219

Mobilization of a transposon in the rice genome. / Nakazaki, Tetsuya; Okumoto, Yutaka; Horibata, Akira; Yamahira, Satoshi; Teraishi, Masayoshi; Nishida, Hidetaka; Inoue, Hiromo; Tanisaka, Takatoshi.

In: Nature, Vol. 421, No. 6919, 09.01.2003, p. 170-172.

Research output: Contribution to journalArticle

Nakazaki, T, Okumoto, Y, Horibata, A, Yamahira, S, Teraishi, M, Nishida, H, Inoue, H & Tanisaka, T 2003, 'Mobilization of a transposon in the rice genome', Nature, vol. 421, no. 6919, pp. 170-172. https://doi.org/10.1038/nature01219
Nakazaki T, Okumoto Y, Horibata A, Yamahira S, Teraishi M, Nishida H et al. Mobilization of a transposon in the rice genome. Nature. 2003 Jan 9;421(6919):170-172. https://doi.org/10.1038/nature01219
Nakazaki, Tetsuya ; Okumoto, Yutaka ; Horibata, Akira ; Yamahira, Satoshi ; Teraishi, Masayoshi ; Nishida, Hidetaka ; Inoue, Hiromo ; Tanisaka, Takatoshi. / Mobilization of a transposon in the rice genome. In: Nature. 2003 ; Vol. 421, No. 6919. pp. 170-172.
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