TY - JOUR
T1 - Mitogenic effects of neurotrophins on a periodontal ligament cell line
AU - Tsuboi, Y.
AU - Nakanishi, T.
AU - Takano-Yamamoto, T.
AU - Miyamoto, M.
AU - Yamashiro, T.
AU - Takigawa, M.
PY - 2001
Y1 - 2001
N2 - Nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), and neurotrophin-3 (NT-3) are three representative neurotrophins responsible for the differentiation and survival of neurons, and their high-affinity receptors are tropomyosin-receptor-kinase (TRK)A, TRKB, and TRKC, respectively. In this study, we investigated the expression of neurotrophins in a mouse periodontal ligament cell line (MPL), by reverse transcription-polymerase chain-reaction (RT-PCR) and enzyme-linked immunoabsorbent assay (ELISA). We also studied the expression of TRK receptors on MPL by immunostaining and the effects of neurotrophins on the proliferation of MPL, with a hypothesis of autocrine mechanism of neurotrophins. Each neurotrophin and TRK receptor was expressed, and neurotrophins enhanced the proliferation of MPL. These findings suggest that the MPL has functional neurotrophin receptors involved in an autocrine function of neurotrophins. The expression level of neurotrophins and TRKs showed the reverse pattern, and we propose an auto-regulatory mechanism of ligands and receptors in accordance with the level of synthesized neurotrophins.
AB - Nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), and neurotrophin-3 (NT-3) are three representative neurotrophins responsible for the differentiation and survival of neurons, and their high-affinity receptors are tropomyosin-receptor-kinase (TRK)A, TRKB, and TRKC, respectively. In this study, we investigated the expression of neurotrophins in a mouse periodontal ligament cell line (MPL), by reverse transcription-polymerase chain-reaction (RT-PCR) and enzyme-linked immunoabsorbent assay (ELISA). We also studied the expression of TRK receptors on MPL by immunostaining and the effects of neurotrophins on the proliferation of MPL, with a hypothesis of autocrine mechanism of neurotrophins. Each neurotrophin and TRK receptor was expressed, and neurotrophins enhanced the proliferation of MPL. These findings suggest that the MPL has functional neurotrophin receptors involved in an autocrine function of neurotrophins. The expression level of neurotrophins and TRKs showed the reverse pattern, and we propose an auto-regulatory mechanism of ligands and receptors in accordance with the level of synthesized neurotrophins.
KW - Neurotrophin
KW - Periodontal ligament cell line
KW - Proliferation
KW - Tropomyosin-receptor-kinase (TRK)
UR - http://www.scopus.com/inward/record.url?scp=0035288080&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0035288080&partnerID=8YFLogxK
U2 - 10.1177/00220345010800030701
DO - 10.1177/00220345010800030701
M3 - Article
C2 - 11379889
AN - SCOPUS:0035288080
SN - 0022-0345
VL - 80
SP - 881
EP - 886
JO - Journal of Dental Research
JF - Journal of Dental Research
IS - 3
ER -