Mitogenic effects of neurotrophins on a periodontal ligament cell line

Y. Tsuboi, T. Nakanishi, T. Takano-Yamamoto, M. Miyamoto, T. Yamashiro, Masaharu Takigawa

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), and neurotrophin-3 (NT-3) are three representative neurotrophins responsible for the differentiation and survival of neurons, and their high-affinity receptors are tropomyosin-receptor-kinase (TRK)A, TRKB, and TRKC, respectively. In this study, we investigated the expression of neurotrophins in a mouse periodontal ligament cell line (MPL), by reverse transcription-polymerase chain-reaction (RT-PCR) and enzyme-linked immunoabsorbent assay (ELISA). We also studied the expression of TRK receptors on MPL by immunostaining and the effects of neurotrophins on the proliferation of MPL, with a hypothesis of autocrine mechanism of neurotrophins. Each neurotrophin and TRK receptor was expressed, and neurotrophins enhanced the proliferation of MPL. These findings suggest that the MPL has functional neurotrophin receptors involved in an autocrine function of neurotrophins. The expression level of neurotrophins and TRKs showed the reverse pattern, and we propose an auto-regulatory mechanism of ligands and receptors in accordance with the level of synthesized neurotrophins.

Original languageEnglish
Pages (from-to)881-886
Number of pages6
JournalJournal of Dental Research
Volume80
Issue number3
Publication statusPublished - 2001

Fingerprint

Periodontal Ligament
Nerve Growth Factors
Cell Line
Nerve Growth Factor Receptors
Neurotrophin 3
Brain-Derived Neurotrophic Factor
Nerve Growth Factor
Reverse Transcription
Ligands
Neurons
Polymerase Chain Reaction
Enzymes

Keywords

  • Neurotrophin
  • Periodontal ligament cell line
  • Proliferation
  • Tropomyosin-receptor-kinase (TRK)

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Tsuboi, Y., Nakanishi, T., Takano-Yamamoto, T., Miyamoto, M., Yamashiro, T., & Takigawa, M. (2001). Mitogenic effects of neurotrophins on a periodontal ligament cell line. Journal of Dental Research, 80(3), 881-886.

Mitogenic effects of neurotrophins on a periodontal ligament cell line. / Tsuboi, Y.; Nakanishi, T.; Takano-Yamamoto, T.; Miyamoto, M.; Yamashiro, T.; Takigawa, Masaharu.

In: Journal of Dental Research, Vol. 80, No. 3, 2001, p. 881-886.

Research output: Contribution to journalArticle

Tsuboi, Y, Nakanishi, T, Takano-Yamamoto, T, Miyamoto, M, Yamashiro, T & Takigawa, M 2001, 'Mitogenic effects of neurotrophins on a periodontal ligament cell line', Journal of Dental Research, vol. 80, no. 3, pp. 881-886.
Tsuboi Y, Nakanishi T, Takano-Yamamoto T, Miyamoto M, Yamashiro T, Takigawa M. Mitogenic effects of neurotrophins on a periodontal ligament cell line. Journal of Dental Research. 2001;80(3):881-886.
Tsuboi, Y. ; Nakanishi, T. ; Takano-Yamamoto, T. ; Miyamoto, M. ; Yamashiro, T. ; Takigawa, Masaharu. / Mitogenic effects of neurotrophins on a periodontal ligament cell line. In: Journal of Dental Research. 2001 ; Vol. 80, No. 3. pp. 881-886.
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