Mitochondrial dynamics in plant male gametophyte visualized by fluorescent live imaging

Ryo Matsushima, Yuki Hamamura, Tetsuya Higashiyama, Shin Ichi Arimura, Sodmergen, Nobuhiro Tsutsumi, Wataru Sakamoto

    Research output: Contribution to journalArticlepeer-review

    38 Citations (Scopus)

    Abstract

    Visualization of organelles in living cells is a powerful method for studying their dynamic behavior. Here we attempted to visualize mitochondria in angiosperm male gametophyte (pollen grain from Arabidopsis thaliana) that are composed of one vegetative cell (VC) and two sperm cells (SCs). Combination of mitochondria-targeted fluorescent proteins with VC- or SC-specific expression allowed us to observe the precise number and dynamic behavior of mitochondria in the respective cell types. Furthermore, live imaging of SC mitochondria during double fertilization confirmed previous observations, demonstrated by electron microscopy in other species, that sperm mitochondria enter into the egg and central cells. We also attempted to visualize mutant mitochondria that were elongated due to a defect in mitochondrial division. This mutant phenotype was indeed detectable in VC mitochondria of a heterozygous F1 plant, suggesting active mitochondrial division in male gametophyte. Finally, we performed mutant screening and isolated a putative mitochondrial protein transport mutant whose phenotype was detectable only in haploid cells. The transgenic materials presented in this work are useful not only for live imaging but also for studying mitochondrial functions by mutant analysis.

    Original languageEnglish
    Pages (from-to)1074-1083
    Number of pages10
    JournalPlant and Cell Physiology
    Volume49
    Issue number7
    DOIs
    Publication statusPublished - Jul 2008

    Keywords

    • Arabidopsis thaliana
    • Fertilization
    • Fluorescent protein
    • Mitochondria
    • Pollen grain
    • Sperm cell

    ASJC Scopus subject areas

    • Physiology
    • Plant Science
    • Cell Biology

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