Mitochondrial dynamics in plant male gametophyte visualized by fluorescent live imaging

Ryo Matsushima, Yuki Hamamura, Tetsuya Higashiyama, Shin Ichi Arimura, Sodmergen, Nobuhiro Tsutsumi, Wataru Sakamoto

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Visualization of organelles in living cells is a powerful method for studying their dynamic behavior. Here we attempted to visualize mitochondria in angiosperm male gametophyte (pollen grain from Arabidopsis thaliana) that are composed of one vegetative cell (VC) and two sperm cells (SCs). Combination of mitochondria-targeted fluorescent proteins with VC- or SC-specific expression allowed us to observe the precise number and dynamic behavior of mitochondria in the respective cell types. Furthermore, live imaging of SC mitochondria during double fertilization confirmed previous observations, demonstrated by electron microscopy in other species, that sperm mitochondria enter into the egg and central cells. We also attempted to visualize mutant mitochondria that were elongated due to a defect in mitochondrial division. This mutant phenotype was indeed detectable in VC mitochondria of a heterozygous F1 plant, suggesting active mitochondrial division in male gametophyte. Finally, we performed mutant screening and isolated a putative mitochondrial protein transport mutant whose phenotype was detectable only in haploid cells. The transgenic materials presented in this work are useful not only for live imaging but also for studying mitochondrial functions by mutant analysis.

Original languageEnglish
Pages (from-to)1074-1083
Number of pages10
JournalPlant and Cell Physiology
Volume49
Issue number7
DOIs
Publication statusPublished - Jul 2008

Fingerprint

Mitochondrial Dynamics
mitochondria
image analysis
Mitochondria
vegetative cells
mutants
spermatozoa
Spermatozoa
cells
phenotype
protein transport
Phenotype
Angiosperms
haploidy
organelles
Haploidy
Mitochondrial Proteins
Angiospermae
electron microscopy
Protein Transport

Keywords

  • Arabidopsis thaliana
  • Fertilization
  • Fluorescent protein
  • Mitochondria
  • Pollen grain
  • Sperm cell

ASJC Scopus subject areas

  • Plant Science
  • Physiology
  • Cell Biology

Cite this

Mitochondrial dynamics in plant male gametophyte visualized by fluorescent live imaging. / Matsushima, Ryo; Hamamura, Yuki; Higashiyama, Tetsuya; Arimura, Shin Ichi; Sodmergen; Tsutsumi, Nobuhiro; Sakamoto, Wataru.

In: Plant and Cell Physiology, Vol. 49, No. 7, 07.2008, p. 1074-1083.

Research output: Contribution to journalArticle

Matsushima, Ryo ; Hamamura, Yuki ; Higashiyama, Tetsuya ; Arimura, Shin Ichi ; Sodmergen ; Tsutsumi, Nobuhiro ; Sakamoto, Wataru. / Mitochondrial dynamics in plant male gametophyte visualized by fluorescent live imaging. In: Plant and Cell Physiology. 2008 ; Vol. 49, No. 7. pp. 1074-1083.
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