Mitf contributes to melanosome distribution and melanophore dendricity

Akiha Kawasaki, Mayuko Kumasaka, Akira Sato, Makoto Suzuki, Koji Tamura, Toshiyasu Goto, Makoto Asashima, Hiroaki Yamamoto

Research output: Chapter in Book/Report/Conference proceedingChapter

16 Citations (Scopus)

Abstract

Mitf is a transcription factor of the basic/helix-loop-helix/leucine-zipper family which is indispensable for development of melanocytes and the retinal pigment epithelium. Our previous work using Xenopus laevis as a model system suggested that Mitf regulates melanosome dispersal in vivo though whether this was via melanosome transport or melanophore dendricity was not obvious. To better understand the role of Mitf, we have now characterized neural tube cultures from wild-type Mitf-injected or a dominant-negative Mitf-injected embryos and compared them with controls. In vitro, lower levels of Mitf activity induced less dendritic melanophores with aggregated melanosomes, whereas melanophores overexpressing Mitf had an extensive dendritic morphology with dispersed melanosomes. Moreover, immunorfluoresence assays reveal that expression of a dominant-negative Mitf leads to decreased Rab27a expression. These results suggest that Mitf is involved in the regulation of melanosome transport and the level of dendricity in melanophores.

Original languageEnglish
Title of host publicationPigment Cell and Melanoma Research
Pages56-62
Number of pages7
Volume21
Edition1
DOIs
Publication statusPublished - Feb 2008
Externally publishedYes

Fingerprint

Basic Helix-Loop-Helix Leucine Zipper Transcription Factors
Melanophores
Melanosomes
Retinal Pigments
Assays
Neural Tube
Retinal Pigment Epithelium
Melanocytes
Xenopus laevis
Embryonic Structures

Keywords

  • Dendricity
  • Melanosome transport
  • Mitf
  • Neural tube culture
  • Xenopus melanophore

ASJC Scopus subject areas

  • Dermatology
  • Oncology
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Kawasaki, A., Kumasaka, M., Sato, A., Suzuki, M., Tamura, K., Goto, T., ... Yamamoto, H. (2008). Mitf contributes to melanosome distribution and melanophore dendricity. In Pigment Cell and Melanoma Research (1 ed., Vol. 21, pp. 56-62) https://doi.org/10.1111/j.1755-148X.2007.00420.x

Mitf contributes to melanosome distribution and melanophore dendricity. / Kawasaki, Akiha; Kumasaka, Mayuko; Sato, Akira; Suzuki, Makoto; Tamura, Koji; Goto, Toshiyasu; Asashima, Makoto; Yamamoto, Hiroaki.

Pigment Cell and Melanoma Research. Vol. 21 1. ed. 2008. p. 56-62.

Research output: Chapter in Book/Report/Conference proceedingChapter

Kawasaki, A, Kumasaka, M, Sato, A, Suzuki, M, Tamura, K, Goto, T, Asashima, M & Yamamoto, H 2008, Mitf contributes to melanosome distribution and melanophore dendricity. in Pigment Cell and Melanoma Research. 1 edn, vol. 21, pp. 56-62. https://doi.org/10.1111/j.1755-148X.2007.00420.x
Kawasaki A, Kumasaka M, Sato A, Suzuki M, Tamura K, Goto T et al. Mitf contributes to melanosome distribution and melanophore dendricity. In Pigment Cell and Melanoma Research. 1 ed. Vol. 21. 2008. p. 56-62 https://doi.org/10.1111/j.1755-148X.2007.00420.x
Kawasaki, Akiha ; Kumasaka, Mayuko ; Sato, Akira ; Suzuki, Makoto ; Tamura, Koji ; Goto, Toshiyasu ; Asashima, Makoto ; Yamamoto, Hiroaki. / Mitf contributes to melanosome distribution and melanophore dendricity. Pigment Cell and Melanoma Research. Vol. 21 1. ed. 2008. pp. 56-62
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