MIP-1α/CCL3-expressing basophil-lineage cells drive the leukemic hematopoiesis of chronic myeloid leukemia in mice

Tomohisa Baba, Yamato Tanabe, Soichiro Yoshikawa, Yoshinori Yamanishi, Soji Morishita, Norio Komatsu, Hajime Karasuyama, Atsushi Hirao, Naofumi Mukaida

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Basophilia is a frequently observed hematological abnormality in chronic myeloid leukemia (CML), but its pathophysiological roles are undefined. We previously demonstrated that an inflammatory chemokine, CCL3, preferentially acts on normal hematopoietic stem/progenitor cells and crucially contributes to the maintenance of leukemia initiating cells (LICs) in bone marrow (BM) during the initiation process of CML. However, the major cellular source of CCL3 in BM and the precise mechanism of CCL3-mediated maintenance of LICs remain to be investigated. To delineate the cellular process facilitating this CCL3-mediated crosstalk between normal and leukemic hematopoiesis, we precisely examined CCL3-expressing cells and their functions in both normal hematopoiesis and CML leukemogenesis. Herein, we demonstrate that basophils can constitutively express CCL3 to negatively regulate the normal hematopoietic process, especially hematopoietic reconstitution after BM transplantation. Moreover, CCL3-expressing basophil-like leukemia cells were found to accumulate in CML BM and supported the predominant expansion of LICs therein. These observations suggest that intra-BM basophil expansion can favor leukemia-tropic hematopoiesis in CML by providing CCL3, a potent inhibitor of normal hematopoiesis and that basophil-derived CCL3 may be a novel target molecule for the treatment of CML.

Original languageEnglish
Pages (from-to)2607-2617
Number of pages11
JournalBlood
Volume127
Issue number21
DOIs
Publication statusPublished - May 26 2016
Externally publishedYes

Fingerprint

Basophils
Hematopoiesis
Leukemia, Myelogenous, Chronic, BCR-ABL Positive
Bone
Leukemia
Bone Marrow
Hematopoietic Stem Cells
Chemokine CCL3
Tropics
Maintenance
Crosstalk
Stem cells
Bone Marrow Transplantation
Bone Marrow Cells
Molecules

ASJC Scopus subject areas

  • Biochemistry
  • Immunology
  • Hematology
  • Cell Biology

Cite this

MIP-1α/CCL3-expressing basophil-lineage cells drive the leukemic hematopoiesis of chronic myeloid leukemia in mice. / Baba, Tomohisa; Tanabe, Yamato; Yoshikawa, Soichiro; Yamanishi, Yoshinori; Morishita, Soji; Komatsu, Norio; Karasuyama, Hajime; Hirao, Atsushi; Mukaida, Naofumi.

In: Blood, Vol. 127, No. 21, 26.05.2016, p. 2607-2617.

Research output: Contribution to journalArticle

Baba, T, Tanabe, Y, Yoshikawa, S, Yamanishi, Y, Morishita, S, Komatsu, N, Karasuyama, H, Hirao, A & Mukaida, N 2016, 'MIP-1α/CCL3-expressing basophil-lineage cells drive the leukemic hematopoiesis of chronic myeloid leukemia in mice', Blood, vol. 127, no. 21, pp. 2607-2617. https://doi.org/10.1182/blood-2015-10-673087
Baba, Tomohisa ; Tanabe, Yamato ; Yoshikawa, Soichiro ; Yamanishi, Yoshinori ; Morishita, Soji ; Komatsu, Norio ; Karasuyama, Hajime ; Hirao, Atsushi ; Mukaida, Naofumi. / MIP-1α/CCL3-expressing basophil-lineage cells drive the leukemic hematopoiesis of chronic myeloid leukemia in mice. In: Blood. 2016 ; Vol. 127, No. 21. pp. 2607-2617.
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