Abstract
Silver-coated capillaries were used for direct sample injection in multiplexed capillary electrophoresis. The absence of an additional electrode simplifies mechanical alignment, reduces contamination, and decreases the amount of sample needed. Capillaries were coated by a silver paint which is a suspension of silver particles in an organic solvent. To provide electrical contact, the upper part of the capillary and a platinum wire were wound together by a copper wire. Electrical resistance from the platinum wire to the tip of the capillary was small enough (7 Ω to 50 Ω) to inject large amounts of DNA samples. Electrokinetic injection from eight separate sample vials to eight capillaries was demonstrated for DNA sequencing by multiplexed capillary electrophoresis. Signal-to-noise ratio and resolution were good enough to call up to 350 base pairs.
| Original language | English |
|---|---|
| Pages (from-to) | 287-290 |
| Number of pages | 4 |
| Journal | HRC Journal of High Resolution Chromatography |
| Volume | 21 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - 1998 |
| Externally published | Yes |
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Keywords
- Capillary electrophoresis
- DNA
- Sample injection
ASJC Scopus subject areas
- Chemical Engineering(all)
Cite this
Miniaturized injection method using silver-coated capillaries in DNA sequencing by multiplexed capillary electrophoresis. / Kaneta, Takashi; Jeong, Hyuk; Yeung, Edward S.
In: HRC Journal of High Resolution Chromatography, Vol. 21, No. 5, 1998, p. 287-290.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Miniaturized injection method using silver-coated capillaries in DNA sequencing by multiplexed capillary electrophoresis
AU - Kaneta, Takashi
AU - Jeong, Hyuk
AU - Yeung, Edward S.
PY - 1998
Y1 - 1998
N2 - Silver-coated capillaries were used for direct sample injection in multiplexed capillary electrophoresis. The absence of an additional electrode simplifies mechanical alignment, reduces contamination, and decreases the amount of sample needed. Capillaries were coated by a silver paint which is a suspension of silver particles in an organic solvent. To provide electrical contact, the upper part of the capillary and a platinum wire were wound together by a copper wire. Electrical resistance from the platinum wire to the tip of the capillary was small enough (7 Ω to 50 Ω) to inject large amounts of DNA samples. Electrokinetic injection from eight separate sample vials to eight capillaries was demonstrated for DNA sequencing by multiplexed capillary electrophoresis. Signal-to-noise ratio and resolution were good enough to call up to 350 base pairs.
AB - Silver-coated capillaries were used for direct sample injection in multiplexed capillary electrophoresis. The absence of an additional electrode simplifies mechanical alignment, reduces contamination, and decreases the amount of sample needed. Capillaries were coated by a silver paint which is a suspension of silver particles in an organic solvent. To provide electrical contact, the upper part of the capillary and a platinum wire were wound together by a copper wire. Electrical resistance from the platinum wire to the tip of the capillary was small enough (7 Ω to 50 Ω) to inject large amounts of DNA samples. Electrokinetic injection from eight separate sample vials to eight capillaries was demonstrated for DNA sequencing by multiplexed capillary electrophoresis. Signal-to-noise ratio and resolution were good enough to call up to 350 base pairs.
KW - Capillary electrophoresis
KW - DNA
KW - Sample injection
UR - http://www.scopus.com/inward/record.url?scp=0031703743&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0031703743&partnerID=8YFLogxK
U2 - 10.1002/(SICI)1521-4168(19980501)21:5<287::AID-JHRC287>3.0.CO;2-#
DO - 10.1002/(SICI)1521-4168(19980501)21:5<287::AID-JHRC287>3.0.CO;2-#
M3 - Article
AN - SCOPUS:0031703743
VL - 21
SP - 287
EP - 290
JO - Journal of Separation Science
JF - Journal of Separation Science
SN - 1615-9306
IS - 5
ER -